Thegl (NM_001017498) Rat Tagged ORF Clone

CAT#: RR214042

LOC498350 (Myc-DDK-tagged ORF) - Rat similar to testicular haploid expressed gene product isoform 2 (LOC498350), (10 ug)

Product Images

Circular map for RR214042

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thegl
• Synonyms: Thegl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214042 representing NM_001017498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAGGAGACTTCTCAGGATCCGAGCCATCTGAGGTGACGGATGGGCAGAACACCAACACCACGA
  TGACGACCGAAACACACACAACCACTGAACACCAACCGAAGCCCCTTGTGGTCAGGCTCCTGGAGGTGCA
  CAACTGTGATGAGGATGAAGAGGATGAGGAGGAAGAACAAGAAGAGGACCACGAAGCGCCCAAGACTCAC
  AAATCCCACGAGGCCTCCGTATCCTTCAAGTCCGTCAACTCCAGTGATTCCCCCAAGGCCAGCGACCTTC
  CCCTGTCCTGCAAGGCCAGTGACTTCCTTGGGTCCTCCAAGGCCAGTGATCCCTCGGAGTCCTTCAAGGC
  CCTTCAGTCATCGGAGAGCCAGGAGGATGTTAAAGATGACTTGTTTTCCAACGCAGTGATTAGGACCTCC
  CCATCTTTGATGACCGGATACCCACCGCAGCTCCAGCTGACTTCCCTGAGAGCACACCCTGCTACCCGTG
  ACCTTGTAAAGAAATGTTTTTATTCTAGAAAGAGAGTTCAAGATCTTTCTAGACCGAAAAAGCAATGGGG
  AACTCCAGATAGGAGGCTGTTTTGGGGAAATCAAGAGCCCATTCGCCCTGTTTCTGAGGCTGCTTTGAAA
  GCTCCGTCGAGCAAGAGAATCGAGGACCTCGCACAGCCCAGGCTGGTCTCCCGACATTATGTCCCTAACA
  GAGTTCAATATTACTACAGCTGCGGAAGAGATTCTGTTATCTGGGAGATCCCTCCTCCTGCACTGATTAC
  CCAGCCTTCCAAACGGATCCAGAAACTGGCCAAACCCAACAGATTCAAAACACAGAGTCTGATAAATAGA
  GAGAACTTACCAGGCACCCTCCGCTATTCCGATCCCTCTCCCCGGATTCTGCGACTCTCCATAGCCAAAG
  GCACAGATCCCAACTACATCCCTCCAAAGACTATTCAAACTAAGATTTCCTTCTCTACCCTGAGTGCGGT
  TGCAAGTCCAAGGATTGTAGACCTTGCCCACCCAAGGATTAAGATAGAGGGTCTATGCTATGAAAGAGAA
  AGGAGTGAATTGCCCATCCGTCCTGTAGCCCCTGCTGCCCTGCTTGCCAATCCTAGTGAAAGAACGATAT
  TTCTAGCAAAGTCCAAACGTGTTCACGAAGACTATCTCCCTGTCCGAGATGCTCGATGGCCTATATCTTA
  TGCTGCGACTCATCCTCAGGTGTCTGAGAGAATCCAGGAACTAGCCAATCCACACACAAGGAGTCCAACG
  CACGTTGTATATTACGACCCGGATGTCTTTAAAGTCAAGCCTTCTGCTTTGAAAGCACATTGTTCCAACA
  GGGTCAAGGAGCTGGCGGAACCCATTGTGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214042 representing NM_001017498
  Red=Cloning site Green=Tags(s)
  
  MEEGDFSGSEPSEVTDGQNTNTTMTTETHTTTEHQPKPLVVRLLEVHNCDEDEEDEEEEQEEDHEAPKTH
  KSHEASVSFKSVNSSDSPKASDLPLSCKASDFLGSSKASDPSESFKALQSSESQEDVKDDLFSNAVIRTS
  PSLMTGYPPQLQLTSLRAHPATRDLVKKCFYSRKRVQDLSRPKKQWGTPDRRLFWGNQEPIRPVSEAALK
  APSSKRIEDLAQPRLVSRHYVPNRVQYYYSCGRDSVIWEIPPPALITQPSKRIQKLAKPNRFKTQSLINR
  ENLPGTLRYSDPSPRILRLSIAKGTDPNYIPPKTIQTKISFSTLSAVASPRIVDLAHPRIKIEGLCYERE
  RSELPIRPVAPAALLANPSERTIFLAKSKRVHEDYLPVRDARWPISYAATHPQVSERIQELANPHTRSPT
  HVVYYDPDVFKVKPSALKAHCSNRVKELAEPIVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017498
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017498.1, NP_001017498.1
• RefSeq Size: 2686 bp
• RefSeq ORF: 1365 bp
• Locus ID: 498350
• UniProt ID: Q5PQR6
• Cytogenetics: 14p11
• MW: 51.2 kDa