Polr3c (NM_001012081) Rat Tagged ORF Clone

CAT#: RR214037

Polr3c (Myc-DDK-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide C (62kD) (Polr3c), (10 ug)

Product Images

Circular map for RR214037

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr3c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214037 representing NM_001012081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCAAGCAGAAATTAAACTGTGCTCTTTGTTGCTACAAGAGCATTTTGGTGAGATTGTAGAAAAAA
  TTGGAGTCCACCTAATCAGAACTGGGAGCCAGCCACTTAGAGTAATTGCCCATGACACAAAAGCATCACT
  GGACCAGGTGAAAAAAGCCCTTTGTGTCCTCATTCACCATAACCTGGTCATCTATCATGTGCATAAACGC
  GGTGTGGTGGAGTATGAAGCCCAATGCAGCCGGGTACTGCGGATGCTTCGGTACCCCCGGTACATCTATA
  CTACCAAAACACTGTATGGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGCTGAATGGCAAAATGAC
  AATGTCGGCTGTTGTGAAGAAAGTAGCAGACCGACTCACAGAGACCATGGAGGATGGCAAGACCATGGAC
  TACGCTGAGGTGTCAAATGCATTTGTTCGACTGGCAGATACTCACTTTGTACAGCGCTGTCCCCTGGTTC
  CTGACACTGACAGTTCTGACCCCGGGCCGCCGCCACCTGCCCCAAACCTTGTCATTAATGAAAAGGACAT
  GTACCTAGTTCCCAAACTGAGCTTGATAGGAAAAGGTAAGAGGAGGAGATCATCTGACGAAGATGCTGCT
  GGAGAGCCCAAGGCCAAGAAACCAAGATGCACAGATAATGAGGAGCCCACACCAGACGATGGGATTTATT
  GGCAAGTCAACCTGGACAGATTCCACCAGCACTTCCGTGACCAAGCAATTGTGAGCGCAGTGGCAAACCG
  AATGGACCAGACAAGCAGCGAGATTGTGCGGACAATGCTCCGAATGAGTGAGATTACCACTCCCTCTGGT
  GCGCCGTTTACTCAGCCACTGTCCTCCAATGAGATCTTCAGATCCCTGCCCGTCGGATATAATATCTCTA
  AACAGGTTCTGGATCAGTACCTCACGCTGCTGGCAGATGACCCACTAGAATTTATTGGAAAGGCTGGCGA
  CAGTGGCGGAGGAATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTATCCACTGCAACTCTGGAG
  TCTGTCATCCAAGAGAGATTCGGGTCTCGCTGTGCCCGGATATTCCGTCTGGTGTTGCAGAAGAAGCACC
  TGGAGCAGAAGCAGGTGGAGGACTTTGCAATGATCCCTGCAAAGGAGGCAAAGGACATGCTGTATAAGAT
  GCTCTCGGAGAACTTCATATCACTGCAGGAAATTCCTAAAACGCCAGACCACGCCCCGTCCAGGACCTTC
  TATCTGTACACCGTGAATGTGCTGTCAGCCGCCAGGATGCTGTTGCACAGGTGCTACAAGAGCATAGCCA
  ACTTGATAGAAAGGCGGCAATTTGAAACAAAAGAGAACAAGCGGCTACTAGAAAAGTCTCAGCGGGTGGA
  AGCCATCATGGCATCAATGCAGGCCACGGGTGCAGAGGAGGTACAGCTGCAGGAGATCGAGGAGATGATC
  ACAGCCCCCGAGCGGCAGCAGCTGGAGACACTGAAACGCAACGTTAACAAGTTGGATGCCAGTGAGATCC
  AGGTAGATGAAACCATCTTCTTACTGGAATCATACATCGAGAGCACCATGAAGAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214037 representing NM_001012081
  Red=Cloning site Green=Tags(s)
  
  MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTKASLDQVKKALCVLIHHNLVIYHVHKR
  GVVEYEAQCSRVLRMLRYPRYIYTTKTLYGDTGELIVEELLLNGKMTMSAVVKKVADRLTETMEDGKTMD
  YAEVSNAFVRLADTHFVQRCPLVPDTDSSDPGPPPPAPNLVINEKDMYLVPKLSLIGKGKRRRSSDEDAA
  GEPKAKKPRCTDNEEPTPDDGIYWQVNLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTPSG
  APFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFIGKAGDSGGGMYVINLHKALASLSTATLE
  SVIQERFGSRCARIFRLVLQKKHLEQKQVEDFAMIPAKEAKDMLYKMLSENFISLQEIPKTPDHAPSRTF
  YLYTVNVLSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIMASMQATGAEEVQLQEIEEMI
  TAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIESTMKRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012081
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012081.1, NP_001012081.1
• RefSeq Size: 2394 bp
• RefSeq ORF: 1602 bp
• Locus ID: 310685
• UniProt ID: Q5XIL3
• Cytogenetics: 2q34
• MW: 60.5 kDa
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF-Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.[UniProtKB/Swiss-Prot Function]