Rsrp1 (NM_001014193) Rat Tagged ORF Clone

CAT#: RR214015

RGD1359529 (Myc-DDK-tagged ORF) - Rat similar to chromosome 1 open reading frame 63 (RGD1359529), (10 ug)

Product Images

Circular map for RR214015

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rsrp1
• Synonyms: RGD1359529
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214015 representing NM_001014193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCGGCCGCCGTGTCCAAGTATGTGAACGACATGTGGCCCGGCTCTCCGCAGGAGAAAGCCTCGC
  CGTCCACCTCGGGCTCGGGCCGCTCCAGCCGCCTGTCTTCGCGCTCCCGCAGCCGCTCTTCGTCCCGCAG
  CTCCAGACCCCACTCCCGCAGCTCCAGCCGCTCGTCCTCGCGGAGCCATAGCCGGCCGCGGAGGAGCAGA
  CGGTCCCGATCCCGATCCCGGTCCCGCAGGCGCCACCAGCGCAAGTACCGGCGATACTCGCGGTCGTACT
  CACGCAGCCGATCGCGGTCCCGCGGCCACCGCTACTACCGAGACAGCCGTTACGAGCAGCCGCGGAGGTA
  CTACCAGTCGCCGTCGCCCTACCGGTCCCGGAGCAGGTCGCGCTCCCGTGGGAGGTCCCATCACCGGAGG
  TCGTACTACGCGATCACTCGAGGGCGGCGCTACTATGGCTTCGGCCGAACCGTGTACCCGGAGGATCGCC
  CCAGGTGGCGGGAAAGATCCAGGACCAGGTCGCGCAGCAGGAGCAGAACCCCTTTTCGCCTGAGTGAGAA
  AGATCGAATGGAGCTGCTAGAAATAGCAAAAGCCAACGCAGCAAAAGCTCTAGGAACAGCCAACTTTGAC
  CTGCCAGCAAGTCTCCGTGCCAAGGAAGCAAGCCAGGGAGCTGTTTCCTGCAGTGGGCCAAAGACGGAGC
  ATTCAGAAAAGCAGACTGAAGTGGGAACTAAAAATGCCAGTGAGAAATCTGCTGCTGCACAGAGAAACAT
  AGCGTTCAGCTCTAATAATTCTGTAGCAAAGCCTCTAGAGAAAACAACGAAAGCTGCTGTTGAAGAGACG
  TCATCAGGATCACCAAAAATAGATAAGAAAAAAAGTCCTTATGGACTGTGGATACCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214015 representing NM_001014193
  Red=Cloning site Green=Tags(s)
  
  MSSAAVSKYVNDMWPGSPQEKASPSTSGSGRSSRLSSRSRSRSSSRSSRPHSRSSSRSSSRSHSRPRRSR
  RSRSRSRSRRRHQRKYRRYSRSYSRSRSRSRGHRYYRDSRYEQPRRYYQSPSPYRSRSRSRSRGRSHHRR
  SYYAITRGRRYYGFGRTVYPEDRPRWRERSRTRSRSRSRTPFRLSEKDRMELLEIAKANAAKALGTANFD
  LPASLRAKEASQGAVSCSGPKTEHSEKQTEVGTKNASEKSAAAQRNIAFSSNNSVAKPLEKTTKAAVEET
  SSGSPKIDKKKSPYGLWIPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014193
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014193.2, NP_001014215.2
• RefSeq Size: 1515 bp
• RefSeq ORF: 903 bp
• Locus ID: 362626
• UniProt ID: Q5U2S0
• Cytogenetics: 5q36
• MW: 34.5 kDa