Dmrta1 (NM_001107945) Rat Tagged ORF Clone

CAT#: RR213863

Dmrta1 (Myc-DDK-tagged ORF) - Rat DMRT-like family A1 (Dmrta1), (10 ug)

Product Images

Circular map for RR213863

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmrta1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213863 representing NM_001107945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAATTTCCTCGTAGAGACCGCAGTGGCGGGTGCCGACCCCATCTGGGCCCAGGGCGGGCTGTTG
  CTCCCGCTTCGGCTGCGCGGTCGGTGTCGTCGGGGATCCCGGTGTCCGCGACTTTCCTAAGGCCGCCTGG
  CCTTTTTCTGCGATCCACCGCAAGTGCGGGTCGCGCAGGATGCGTTCCGGGTCCTGGGCTGGATAGGGCG
  TTGGGGGCGGTGGGCTGCGGCTACCCGCGGACCCCGAAGTGCGCCCGTTGTCGCAACCATGGCGTGGTGT
  CAGCGCTCAAGGGCCACAAGCGCTTCTGCCGCTGGCGGGACTGCGCGTGTGCCAAGTGCACCCTGATCGC
  CGAGCGCCAGCGTGTCATGGCCGCGCAGGTGGCGCTGCGCAGGCAGCAGGCGCAGGAGGAGAGCGAGGCC
  CGTGGGCTGCACAGGCTCCTGTACCAGGGGCCATCGGGGCCCGGGGCTCCAGCCTCGGGGGGCAGCGGCA
  GGACCGAGAGTCCCCAGGTTTTGAGTAGCCCCATGACTGTAGCTGCTCTGGGAGCGGGTGCCTCTAGACA
  CCCTGGGAGCCGGTCTGTCCCAGCTTTCGAGGTTTTCCAGCAAGATTTTGCTGAGAGAAAGCAGGAACCA
  AAACAGAGTAACTGTGAGTCATGCCAGAGTAGACACGAAGAACCAGTCTCCAACACTCATCACCGTTCTC
  CGGGATCATCTAACGGTAATGTTACCATGGAGAAACAGGGCTTCATGTCATCTATTTCAGAACAGTCGGA
  CAAATCGACCATCATCCTGTCTCCTTGCCCCACGGATCAGTCAGGAGGAGAAGACAGCCCCAGGTCCTTC
  TCTTCCTCTGACCTGGAATCAGGGAATGAAAGCGAGTGGGCCAGAGACTACACTGCTACCACAGCCAGCC
  TTTCCACGGTGACCTCAAGACCACGAGACCCTCTCGGAATTCTTACCAGGATTTTTCCAGGTTACAAGCG
  TAGCCGCTTAGAAGGCATCCTACAGTTCTGCAAGGGAGATGTAGTCCAAGCCATTGAACAGATCTTAAAT
  GGGAAAGAACATAAGCCAGACTGCAGGGACCTAGCACATGCAGACTTGGAAAATGCAGCCTTTCAGAGGG
  CTTCAGATTTTAGTCTTGCTGGCATTGGCTTTGGGACTCTAAATAATAAATCAGCTCTCTCTCCTCTTGA
  AGCTGCGTCTGCTGCTTACGGAGGAGATTCATCTCTCTACAGTTTCAATCCTAGGCTAGCTTTCAGCCCA
  CTGCGCCTGGCATATTCTTCTCCAGGAAGAGCACTGTCTGGTTTTATGTCGCCTTACCTAACGCCTGGAC
  TCGTTCCAGCATTGCCTTTTCGGCCAACTTTGGATTATGCATTTCCAGGGATGATTAGGGAGCCGCCCCA
  CCTTCCTAGCAAACATTTAGTAACTGGTGGTAGACTGTATTTCAGGCCCAATCAGGAGCACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213863 representing NM_001107945
  Red=Cloning site Green=Tags(s)
  
  MEQFPRRDRSGGCRPHLGPGRAVAPASAARSVSSGIPVSATFLRPPGLFLRSTASAGRAGCVPGPGLDRA
  LGAVGCGYPRTPKCARCRNHGVVSALKGHKRFCRWRDCACAKCTLIAERQRVMAAQVALRRQQAQEESEA
  RGLHRLLYQGPSGPGAPASGGSGRTESPQVLSSPMTVAALGAGASRHPGSRSVPAFEVFQQDFAERKQEP
  KQSNCESCQSRHEEPVSNTHHRSPGSSNGNVTMEKQGFMSSISEQSDKSTIILSPCPTDQSGGEDSPRSF
  SSSDLESGNESEWARDYTATTASLSTVTSRPRDPLGILTRIFPGYKRSRLEGILQFCKGDVVQAIEQILN
  GKEHKPDCRDLAHADLENAAFQRASDFSLAGIGFGTLNNKSALSPLEAASAAYGGDSSLYSFNPRLAFSP
  LRLAYSSPGRALSGFMSPYLTPGLVPALPFRPTLDYAFPGMIREPPHLPSKHLVTGGRLYFRPNQEHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107945
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107945.1, NP_001101415.1
• RefSeq Size: 4030 bp
• RefSeq ORF: 1467 bp
• Locus ID: 313352
• Cytogenetics: 5q32
• MW: 52.3 kDa