Ccdc117 (NM_001017502) Rat Tagged ORF Clone

CAT#: RR213794

Ccdc117 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 117 (Ccdc117), (10 ug)

Product Images

Circular map for RR213794

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc117
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213794 representing NM_001017502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCTCGGCCGGCCCTTCAGCGGCCTCCCTCTGAGCGGCAGCGCGGACTTCCTGCAGTCGCCGC
  CGGCCTTCGCGGGCCGGGCCTTCCCGCCAGGAGCCGCCGGCCATGACCTGGCCCCGCGGCCGGGTGTCCG
  CGGCCCGCCGAGCAGCCCGGACGGTAGAACAGCGCGCGGACGTGTTTCCATTCACTGTAGAAAGAAACAC
  AAGCGATTGGCGGAGGACGATGAGTGTCCAGTAAGAAAGAAGCGCTTGACTGAAGCAGAACTTGGTGCAG
  TTGCCGATGAGTGGGTTCTTGGCACACATCAGGGTATAGAAGGTCATGGGGTAAACACGTGTCCTGGTGG
  CCTTTCTGTGCCTAGCATCCTAGATGCTGTCTGTGAAGAAATGGATCAGACAACTGGAGAACCACAGTGT
  GAAGTTGCCCGAAGAAGGCTTCAAGAGATCGAGGACAGGATAATTGACGAAGATGAAGAGGTTGAAAGTG
  ACAGAAATGTTAGCCACCTCCCAAGTCTTGTCCTTTCTGATACCATGAAAACGGGTTTGAAGAGGGAATT
  TGATGAAGTCTTCACAAAGAAGATGATTGAGTCTATGAGCCGTCCTTCTATGGAGCTTGTGCTCTGGAAA
  CCTCTCCCTGAACTCCTTTCTGAGAAGCCAAAGCCATCATCTAACCCTAAGAACTACATGGGAGAGAGCC
  AAACGAAGCACACAGCTACGGGCACTGCCTTCCCACAGAGAACTGAAGTGTTACTGGAACCTCAGTGCAC
  AGACACACCGCTTTACCATAGTCTGGAGACAGCTGCTAGCACAGAGGAAGAGATGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213794 representing NM_001017502
  Red=Cloning site Green=Tags(s)
  
  MAALGRPFSGLPLSGSADFLQSPPAFAGRAFPPGAAGHDLAPRPGVRGPPSSPDGRTARGRVSIHCRKKH
  KRLAEDDECPVRKKRLTEAELGAVADEWVLGTHQGIEGHGVNTCPGGLSVPSILDAVCEEMDQTTGEPQC
  EVARRRLQEIEDRIIDEDEEVESDRNVSHLPSLVLSDTMKTGLKREFDEVFTKKMIESMSRPSMELVLWK
  PLPELLSEKPKPSSNPKNYMGESQTKHTATGTAFPQRTEVLLEPQCTDTPLYHSLETAASTEEEMEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017502
• ORF Size: 831 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017502.1, NP_001017502.1
• RefSeq Size: 2224 bp
• RefSeq ORF: 834 bp
• Locus ID: 498404
• UniProt ID: Q5M9G5
• Cytogenetics: 14q21
• MW: 30.4 kDa