Fgf12 (NM_130814) Rat Tagged ORF Clone

CAT#: RR213375

Fgf12 (Myc-DDK-tagged ORF) - Rat fibroblast growth factor 12 (Fgf12), (10 ug)

Product Images

Circular map for RR213375

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgf12
• Synonyms: Fhf1; Fhf1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213375 representing NM_130814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAACAGCGACCGGG
  TGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTGTGCGAGAGGCACGTCCTCGG
  GGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGGCCAGTGAGGCGGAGACCAGAACCCCAGCTG
  AAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGATATTTCCTGCAGATGCATCCAGATGGTACCATTG
  ATGGGACCAAGGACGAAAACAGCGACTACACCCTCTTCAATCTAATTCCTGTGGGACTGCGTGTGGTGGC
  CATCCAAGGTGTGAAAGCCAGCCTTTATGTGGCCATGAATGGAGAAGGCTATCTCTACAGCTCAGATGTT
  TTTACCCCAGAATGCAAATTTAAGGAGTCTGTGTTTGAAAACTACTATGTGATCTATTCCTCAACCCTGT
  ATCGCCAGCAGGAATCAGGCCGCGCATGGTTTCTAGGACTCAATAAAGAAGGTCAAATCATGAAGGGGAA
  CAGAGTGAAGAAAACCAAGCCCTCATCTCATTTTGTACCAAAACCTATTGAAGTGTGTATGTACAGAGAA
  CCATCTCTACATGAAATTGGAGAAAAGCAAGGACGCTCGAGGAAAAGTTCCGGAACACCCACCATGAATG
  GAGGCAAAGTCGTGAATCAAGATTCTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213375 representing NM_130814
  Red=Cloning site Green=Tags(s)
  
  MAAAIASSLIRQKRQARESNSDRVSASKRRSSPSKDGRSLCERHVLGVFSKVRFCSGRKRPVRRRPEPQL
  KGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDV
  FTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYRE
  PSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130814
• ORF Size: 729 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130814.1, NP_570827.1
• RefSeq Size: 732 bp
• RefSeq ORF: 732 bp
• Locus ID: 170630
• UniProt ID: P61150
• Cytogenetics: 11q22
• MW: 27.4 kDa
• Gene Summary: member of the fibroblast growth factor (FGF) family; implicated in activities including embryonic development, cell growth, morphogenesis, tissue growth, and tumor growth and invasion [RGD, Feb 2006]