Setsip (NM_194353) Rat Tagged ORF Clone

CAT#: RR213325

Setl1 (Myc-DDK-tagged ORF) - Rat SET translocation-like 1 (Setl1), (10 ug)

Product Images

Circular map for RR213325

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Setsip
• Synonyms: RGD727855; Set; Setl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213325 representing NM_194353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGAAGCGGCAATCTGCGATCCCGCCTCAGCCCAAGAAACCCAGACCCATTGCTGCCCCGAAGT
  TGGAGGACAAGTTGGCCTCTCCCAGCCTGCCGAAGGGAGAAAAAGAACAGCAAGAAACTATTGACCATAT
  TGATGAAGTACAAAATGAAATGGACAGACTTAATGAACAACCCAGTGAGGAAATTTTGAAAGTAGAACAA
  AAATATAACAGACTCTGCCAACCATTTTTTTTTCAGAAGAGGTCAGAATTGATCGCCAAAATCCCAAATT
  TTTGGGTAACAACATTTGTCAACCATCCACAAGTGTCTGCACCGCGTGGGGAGGAAGACGAGGAGGCCCT
  GCATTATTTGACCAGAGTTGAAGGGACAGAATTTGGAGACATTAAATCAGGTTACAGAATAGATTTTTAT
  TTTGATGAGAATCCTTACTTTGAAAATAAAGTTCTCTCCAAAGAATTTCATCTGAATGAGAGCGGTGACC
  CGTCTTCAAAGTCCACCGAAATCAAATGGAAATCTGGGAAGGATTTGACAAAATGCTCAAGTCAAACGCC
  GAATAAGGCCAGCAGGAAGAGGCAGCGTGAAAAGCCAGAGAGTTCCTTTCCCTGGTTTACTGACCGTTCT
  GACGCAGGTGCTGACGAGTTAGGAGAGGTCGTCAAGGATAATATTTGGCCAAACCCCTTGCGGTCCTATT
  TGGTTCCCGATATGGGTGATGAAGGAGAGGCAGAGGATGATGATGATGACGACGACGAAGAAGAACCGTT
  GGAAGATATTGATGAAGGAGACAGGGATGAAGGTGAAGAAGATGACGATGAGGAGGAAGGGGAGGAAGGA
  GGACGAAGGCGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213325 representing NM_194353
  Red=Cloning site Green=Tags(s)
  
  MAPKRQSAIPPQPKKPRPIAAPKLEDKLASPSLPKGEKEQQETIDHIDEVQNEMDRLNEQPSEEILKVEQ
  KYNRLCQPFFFQKRSELIAKIPNFWVTTFVNHPQVSAPRGEEDEEALHYLTRVEGTEFGDIKSGYRIDFY
  FDENPYFENKVLSKEFHLNESGDPSSKSTEIKWKSGKDLTKCSSQTPNKASRKRQREKPESSFPWFTDRS
  DAGADELGEVVKDNIWPNPLRSYLVPDMGDEGEAEDDDDDDDEEEPLEDIDEGDRDEGEEDDDEEEGEEG
  GRRRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194353
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194353.1, NP_919334.1
• RefSeq Size: 858 bp
• RefSeq ORF: 858 bp
• Locus ID: 290432
• Cytogenetics: 15q21
• MW: 32.8 kDa
• Gene Summary: protein phosphatase regulator protein; human homolog appears to be involved in histone acetylation [RGD, Feb 2006]