Gja1 (NM_012567) Rat Tagged ORF Clone

CAT#: RR213232

Gja1 (Myc-DDK-tagged ORF) - Rat gap junction protein, alpha 1 (Gja1), (10 ug)

Product Images

Circular map for RR213232

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gja1
• Synonyms: Cx43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213232 representing NM_012567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACTGGAGTGCCTTGGGGAAGCTTCTGGACAAGGTCCAAGCCTACTCCACCGCTGGAGGGAAGG
  TGTGGCTGTCAGTGCTCTTCATATTCAGAATCCTGCTCCTGGGGACAGCTGTTGAGTCAGCTTGGGGTGA
  TGAACAGTCTGCCTTTCGCTGTAACACTCAACAACCTGGCTGCGAAAACGTCTGCTATGACAAGTCCTTC
  CCCATCTCTCACGTGCGCTTCTGGGTCCTTCAGATCATATTCGTGTCTGTGCCCACCCTCCTGTACTTGG
  CCCATGTGTTCTATGTGATGAGGAAGGAAGAGAAGCTAAACAAGAAAGAAGAGGAGCTCAAAGTGGCCCA
  GACTGACGGGGTCAACGTGGAGATGCACCTGAAGCAGATTGAAATCAAGAAGTTCAAGTACGGGATTGAA
  GAGCACGGCAAGGTGAAAATGAGGGGCGGCTTGCTGAGAACCTACATCATCAGCATCCTCTTCAAGTCTG
  TCTTCGAGGTGGCCTTCCTGCTCATCCAGTGGTACATCTATGGGTTCAGCTTGAGCGCGGTCTACACCTG
  CAAGAGAGATCCCTGCCCCCACCAGGTAGACTGCTTCCTCTCACGTCCCACGGAGAAAACCATCTTCATC
  ATCTTCATGCTGGTGGTGTCCTTGGTGTCTCTCGCTTTGAACATCATTGAGCTCTTCTACGTCTTCTTCA
  AAGGCGTTAAGGATCGCGTGAAGGGAAGAAGCGATCCTTACCACGCCACCACTGGCCCACTGAGCCCATC
  AAAAGACTGCGGATCTCCAAAATACGCCTACTTCAATGGCTGCTCCTCACCAACGGCTCCACTCTCGCCT
  ATGTCTCCTCCTGGGTACAAGCTGGTTACTGGTGACAGAAACAATTCCTCGTGCCGCAATTACAACAAGC
  AAGCTAGCGAGCAAAACTGGGCGAACTACAGCGCAGAGCAAAATCGCATGGGGCAGGCCGGAAGCACCAT
  CTCCAACTCGCACGCCCAGCCGTTCGATTTCCCCGACGACAACCAGAATGCCAAAAAAGTTGCTGCTGGA
  CATGAACTCCAGCCATTAGCCATCGTGGACCAACGACCTTCCAGCAGAGCCAGCAGCCGCGCCAGCAGCA
  GGCCTCGGCCTGATGACCTGGAGATTGGCCTCGGCCTGATGACCTGGAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213232 representing NM_012567
  Red=Cloning site Green=Tags(s)
  
  MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSF
  PISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIE
  EHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFI
  IFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSP
  MSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAG
  HELQPLAIVDQRPSSRASSRASSRPRPDDLEIGLGLMTWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012567
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3148 bp
• RefSeq ORF: 1149 bp
• Locus ID: 24392
• UniProt ID: P08050
• Cytogenetics: 20q11
• MW: 43 kDa
• Gene Summary: gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]