Nhlrc1 (NM_199236) Rat Tagged ORF Clone

CAT#: RR213175

Nhlrc1 (Myc-DDK-tagged ORF) - Rat NHL repeat containing 1 (Nhlrc1), (10 ug)

Product Images

Circular map for RR213175

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhlrc1
• Synonyms: Epm2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213175 representing NM_199236
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGGAGGCAGCGGGGGTGCGGCCCGAGCTGGTGCGCGAGGCGGAGGTCAGCCTGCTGGAGTGCA
  AGGTGTGCTTCGAGAGGTTCGGCCACCGGCAGCAGCGGCGCCCGCGCAACCTGCCCTGCGGCCACGTGGT
  CTGCTTGGCCTGCGTCGCCGCCCTCGCGCACCCGCGGACGCTGGCCCTCGAGTGTCCCTTCTGCCGGCGG
  GCCTGCCGAGCTTGTGACACCAGCGATTGCCTGCCGGTGCTGCACCTTCTGGAGCTCCTGGGCTCCACCC
  TCCACGCGTCCCCGGCTGCCCTCAGCGCCGCCTCCTGTGCGCCCGGGGCTCTCACCTGCTACCATGCCTT
  TGGCGGGTGGGGGACCCTAGTGAACCCCACGGGGCTTGCACTGTGCCCCAAGACCGGACGGGTAGTAGTC
  GTGCACGACGGTAAGAGACGGGTCAAGATCTTCGATTCCGGAGGAGGAGGTGCACACCAGTTTGGAGAGA
  AGGGGGACGCAGCGCATGACGTGAAGTACCCACTGGATGTCGCGGTCACCAACGACTGCCATGTGGTTGT
  CACCGACGCCGGCGACTGCTCCCTCAAAGTGTTTGATTTCTTTGGCCAGATCAAGCTGGTTGTGGGAAAG
  CAGTTTTCCCTGCCTTGGGGTGTAGAGATAACCCCTCACAACGGGGTCCTGGTGACTGACGCAGAGGCAG
  GGACTTTGCACCTGCTGGAAGCGGATTTCCCTGAAGGGGTCCTTCGGAGAATTGAGAGGTTGCAAGCTCA
  CCTGTGTAATCCCCGTGGGGTGGCAGTGTCATGGCTCACCGGGGCCATCGCGGTCCTAGAGCATCCTTGT
  GCCTTGGGGACGTCAAGCGGTAACAACACAAGGGTGAAGGTGTTCAACTCCAGTATGCAGCTGATTGGCC
  AGGTGGACAGCTTCGGACTGAACCTCCTCTTCCCCTCCAAAATAACCGCCTCAGCTGTGACCTTCGATCA
  CCAAGGAAACGTGATTGTTGCTGATACCTCTGGGCCAGCCATCGTATGCTTGGGGAAACCTGAGGAATTT
  CCAGCCCTGAAGCCTATGGTTACTCATGGCCTTTCCCGTCCTGTGGCACTGGTCTTCACCAAGGAGAATT
  CTCTTCTTGTGCTGGATTCTGCATCCCATTCTATAAAAGTCTTTAAAGTGATGGAGGGTAATGGAGGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213175 representing NM_199236
  Red=Cloning site Green=Tags(s)
  
  MGEEAAGVRPELVREAEVSLLECKVCFERFGHRQQRRPRNLPCGHVVCLACVAALAHPRTLALECPFCRR
  ACRACDTSDCLPVLHLLELLGSTLHASPAALSAASCAPGALTCYHAFGGWGTLVNPTGLALCPKTGRVVV
  VHDGKRRVKIFDSGGGGAHQFGEKGDAAHDVKYPLDVAVTNDCHVVVTDAGDCSLKVFDFFGQIKLVVGK
  QFSLPWGVEITPHNGVLVTDAEAGTLHLLEADFPEGVLRRIERLQAHLCNPRGVAVSWLTGAIAVLEHPC
  ALGTSSGNNTRVKVFNSSMQLIGQVDSFGLNLLFPSKITASAVTFDHQGNVIVADTSGPAIVCLGKPEEF
  PALKPMVTHGLSRPVALVFTKENSLLVLDSASHSIKVFKVMEGNGG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199236
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199236.1, NP_954706.1
• RefSeq Size: 1191 bp
• RefSeq ORF: 1191 bp
• Locus ID: 364682
• UniProt ID: Q6IMG5
• Cytogenetics: 17p14
• MW: 42.1 kDa
• Gene Summary: human homolog is a putative E3 ubiquitin ligase associated with Lafora progressive myoclonus epilepsy and possibly involved in clearance of polyglucosans from dendrites [RGD, Feb 2006]