Pecam1 (NM_031591) Rat Tagged ORF Clone

CAT#: RR213090

Pecam1 (Myc-DDK-tagged ORF) - Rat platelet/endothelial cell adhesion molecule 1 (Pecam1), (10 ug)

Product Images

Circular map for RR213090

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pecam1
• Synonyms: CD31; Pecam
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213090 representing NM_031591
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGGCTCTGCTGCTCACCATGCTGCTCTATGCAAGCCTCCAGGCTCAGGAAAACTCTTTCACCA
  TTAACAGCATCCATATGGAAAGTCGGCCCTCCTGGGAGGTATCGAATGGGCAGAAACTGACCCTGCAGTG
  CCTTGTCGATATCAGCACCACCTCGAAATCTAGGCCTCAGCACCAGGTGCTATTCTATAAGGACGATGCG
  CTGGTGTACAACGTCTCCTCCAGTGAGCACACAGAGAGCTTCGTCATTCCTCAGTCTCGGGTCTTCCACG
  CCGGGAAGTACAAATGCACCGTGATACTGAACAGCAAGGAAAAAACCACAATTGAGTACCAGCTGACGGT
  GAACGGTGTACCCATGCCTGAGGTGACCGTGGACAAAAAGGAGGTGACAGAAGGTGGGATTGTGACAGTC
  AATTGCTCCATGCAAGAAGAAAAGCCACCAATATATTTTAAAATTGAAAAAGTGGAACTGGGGACAAAGA
  ACGTCAAGCTAAGTAGAGAGAAGACCTCCAACATGAACTTTGTGCTCATAGAATTCCCCATCGAGGAGCA
  AGACCACCTGTTAGTGTTTCGCTGCCAAGCTGGCGTCCTGTCCGGAATCAAAATGCAGACCTCAGAATTC
  ATCCGGAGTGAATACGTCACTGTGCAGGAGTTCTTCTCTACACCCAAGTTTCAAATCCAGCCTCCTGAGA
  TGATCATAGAAGGAAACCAGCTGCACATCAAGTGCAGCGTGCAGGTGGCACACTTGGCCCAGGAATTTCC
  AGAAATTATAATCCAGAAAGACAAGGCGATCGTGGCCACCTCCAAGCAAAGCAAAGAAGCTGTCTACTCA
  GTCATGGCCTTGGTAGAGCACAGTGGACACTACACGTGCAAAGTGGAATCGAACCGCATCTCCAAGGCCA
  GTAGCATCCTGGTCAACATAACAGAACTGTTTCCCAGGCCGAAGCTAGAACTCTCCTCCAGCCGTCTGGA
  CCAAGGGGAAATGCTGGACCTGTCCTGCTCTGTCTCGGGTGCGCCTGTAGCTAACTTCACCATCCAGAAG
  GAAGAGACGGTGTTGTCACAGTATCAGAATTTCAGCAAGATTGCCGAGGAGAGGGACAGCGGGCTGTACA
  GCTGTACAGCAGGCATCGGCAAAGTGGTCAAGAGAAGCAATTTGGTACCCGTCCAGGTGTGCGAAATGCT
  CTCCAAACCCCGGATTTTTCATGATGCCAAATTTGAGATCATAAAAGGACAAATCATAGGTATCAGCTGC
  CAGTCAGTAAATGGGACTGCGCCCATCACTTACCGCCTCCTAAGAGCAAAGAGCAACTTCCAGACCGTCC
  AGAAGAACTCCAATGACCCAGTGACATTCACAGACAAGCCCACCAGAGACATGGAATACCAGTGTATAGT
  GGATAATTGCCACTCTCACCCCGAGGTGCGCAGCGAGATCCTGAGGGTCAAGGTAATAGCCCCGGTGGAT
  GAAGTTACGATCTCCATCCTGTCGGGTAACGATGTACAGTCTGGAGATGAAATGGTGCTTCGGTGCTCTG
  TGAAAGAGGGGACTGGCCCTGTCACGTTTCAGTTTTACAAAGAAAAGGAGGGCAGACCCTTCCACGAAGA
  AACCGTGAATGACACCCAAGTGTTTTGGCACCATGAACAAACTAGCAAGGAGCAGGAAGGCCAGTACTAT
  TGTACAGCGTTCAACAGAGCCAGCATTGTGACCAGTCTCCGAAGCGGCCCTCTAACAGTCAGAGTCTTTC
  TTGCTCCGTGGAAGAAAGGGCTCATTGCAGTCGTTGTCATTGGAGTGGTCATTGCCGCCTTGATAGTTGC
  AGCCAAATACTACTTCCTGAGGAAAGCCAAGGCCAAGCAGAAGCCCGTGGAGATGTCCAGGCCAGCAGTC
  CCACTTCTGAACTCCAACAGTGAGAAGGTTTCTGAGCCCAGTGTGGAAACCAACAGCCATTACGACTCCC
  AGAACATGGATGTGGAGTACACAGAAGTGGAAGTGTCCTCCCTTGAGCCTCACCAAGAGAACGGAAGGCT
  CCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213090 representing NM_031591
  Red=Cloning site Green=Tags(s)
  
  MLLALLLTMLLYASLQAQENSFTINSIHMESRPSWEVSNGQKLTLQCLVDISTTSKSRPQHQVLFYKDDA
  LVYNVSSSEHTESFVIPQSRVFHAGKYKCTVILNSKEKTTIEYQLTVNGVPMPEVTVDKKEVTEGGIVTV
  NCSMQEEKPPIYFKIEKVELGTKNVKLSREKTSNMNFVLIEFPIEEQDHLLVFRCQAGVLSGIKMQTSEF
  IRSEYVTVQEFFSTPKFQIQPPEMIIEGNQLHIKCSVQVAHLAQEFPEIIIQKDKAIVATSKQSKEAVYS
  VMALVEHSGHYTCKVESNRISKASSILVNITELFPRPKLELSSSRLDQGEMLDLSCSVSGAPVANFTIQK
  EETVLSQYQNFSKIAEERDSGLYSCTAGIGKVVKRSNLVPVQVCEMLSKPRIFHDAKFEIIKGQIIGISC
  QSVNGTAPITYRLLRAKSNFQTVQKNSNDPVTFTDKPTRDMEYQCIVDNCHSHPEVRSEILRVKVIAPVD
  EVTISILSGNDVQSGDEMVLRCSVKEGTGPVTFQFYKEKEGRPFHEETVNDTQVFWHHEQTSKEQEGQYY
  CTAFNRASIVTSLRSGPLTVRVFLAPWKKGLIAVVVIGVVIAALIVAAKYYFLRKAKAKQKPVEMSRPAV
  PLLNSNSEKVSEPSVETNSHYDSQNMDVEYTEVEVSSLEPHQENGRLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031591
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031591.1, NP_113779.1
• RefSeq Size: 3244 bp
• RefSeq ORF: 2037 bp
• Locus ID: 29583
• UniProt ID: Q3SWT0
• Cytogenetics: 10q32.1
• MW: 76.2 kDa
• Gene Summary: cell adhesion molecule, also intracellular signaling molecule; may mediate leukocyte adhesion and migration, angiogenesis, and thrombosis [RGD, Feb 2006]