Ap2m1 (NM_053837) Rat Tagged ORF Clone

CAT#: RR212640

Ap2m1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, mu 1 subunit (Ap2m1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap2m1
• Synonyms: Ap50; Clapm1; mu2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212640 representing NM_053837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTTATCTCCCGAGTCTACAGAGATGACA
  TCGGGAGGAATGCAGTGGATGCCTTTCGCGTCAATGTCATTCACGCACGGCAGCAGGTGCGCAGCCCTGT
  CACAAACATCGCTCGCACCAGCTTCTTCCATGTTAAGCGGTCCAACATCTGGCTGGCAGCAGTCACCAAG
  CAGAATGTCAATGCCGCCATGGTCTTCGAATTCCTCTACAAGATGTGTGATGTAATGGCTGCTTACTTTG
  GCAAGATCAGCGAGGAGAACATCAAGAACAATTTCGTGCTCATATACGAGCTGCTGGACGAGATTCTGGA
  CTTTGGCTACCCACAGAACTCAGAGACAGGTGCACTGAAAACCTTCATCACCCAACAGGGTATCAAGAGT
  CAGCATCAGACAAAAGAAGAGCAGTCCCAGATCACCAGCCAGGTGACCGGGCAAATTGGCTGGAGGCGGG
  AAGGCATCAAGTATCGTCGGAATGAACTCTTCCTAGATGTTCTGGAGAGTGTGAACCTGCTTATGTCCCC
  ACAGGGGCAGGTGCTGAGTGCCCATGTGTCGGGCCGGGTGGTGATGAAGAGTTACCTGAGCGGCATGCCT
  GAGTGCAAGTTTGGAATGAATGACAAGATTGTCATAGAAAAGCAGGGCAAAGGCACAGCCGATGAAACAA
  GCAAGAGTGGTAAGCAGTCGATCGCCATTGATGACTGCACCTTTCACCAGTGTGTGCGACTCAGCAAGTT
  TGACTCTGAGCGGAGCATCAGCTTTATCCCTCCTGATGGAGAGTTTGAACTCATGAGATACCGTACTACC
  AAGGACATCATCCTGCCTTTCCGGGTGATCCCATTGGTGCGGGAGGTGGGACGCACCAAACTGGAGGTCA
  AGGTGGTCATCAAGTCCAACTTCAAGCCCTCACTTCTGGCCCAGAAGATTGAGGTGAGGATCCCAACCCC
  ATTGAACACAAGCGGGGTGCAAGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAACGCC
  ATTGTATGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCACAGATCAGCGCAGAGATTGAGCTCCTGC
  CCACCAATGATAAGAAGAAATGGGCTCGGCCCCCCATTTCCATGAACTTTGAGGTGCCATTTGCGCCTTC
  TGGCCTCAAGGTGCGCTACTTGAAGGTATTTGAGCCGAAGCTGAACTACAGCGACCACGATGTCATCAAA
  TGGGTGCGCTACATTGGCCGAAGTGGCATTTACGAAACCCGCTGCTGGGTGCGCTACATTGGCCGAAGTG
  GCATTTACGAAACCCGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212640 representing NM_053837
  Red=Cloning site Green=Tags(s)
  
  MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTK
  QNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKS
  QHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMP
  ECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTT
  KDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENA
  IVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIK
  WVRYIGRSGIYETRCWVRYIGRSGIYETRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053837
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1816 bp
• RefSeq ORF: 1308 bp
• Locus ID: 116563
• UniProt ID: P84092
• Cytogenetics: 11q23
• MW: 49.7 kDa
• Gene Summary: encodes the mu subunit of the heterotetrameric coat assembly protein complex 2 (AP2); associated with clathrin-coated vesicles and may be important for intracellular trafficking [RGD, Feb 2006]