Mtrf1l (NM_001025723) Rat Tagged ORF Clone

CAT#: RR212532

Mtrf1l (Myc-DDK-tagged ORF) - Rat mitochondrial translational release factor 1-like (Mtrf1l), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR212532

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtrf1l
• Synonyms: MGC116100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212532 representing NM_001025723
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGCGGGTTTCTGCGCAGTGCCCGGCGCCTGTGGGCCCGCCGCGCCATCAGCCGCATGCCACCGC
  CTTCCGAGGAGCTCCTCGCCCGCGGCGGGCCCCTGCGAGCCTTCCTGGAGCGCCGCGTGGGCTCGGAGGC
  CGGCGGTCTGGACGCTGGGAGTCCCCAGCTTGCCGCGGCGGCCCGGCTGCTGAACGAGAAGGAGCGGGAG
  CTACGGGACACTGAATCCTTGCTGCACGATGAGAATGAAGATTTGAAGAAACTCGCAGAGAGTGAGATAG
  CTTTGTGTCAAAAAGAAATAGCCGAGCTGAAGCATCGGATCATCTCCCTTTTGGTTCCTTCAGAAGACAT
  GGATGGCAGTGACCTGATCCTGGAGGTGACTGCGGGTGTGGGTGGTCAGGAAGCCATGCTTTTCACTTCT
  GAGATGTTTGACATGTACCAGCAATACGCTGCATTTAAAAGATGGCATTTTGAAACACTGGAATATTTCC
  CAAGTGAACTAGGTGGCCTCAGACATGCATCAGCCAGCATCGGAGGTCCAGAAGCCTACAGACACATGAA
  ATTTGAAGGAGGAGTACATAGAGTACAGAGAGTACCAAAGACAGAGAGGCAAGGCCGCATCCACACCAGC
  ACCATGACGGTGGCAATTCTGCCCCAGCCTACTGAGATTAAACTGGTGATTAACCCTAAAGATTTGAGAA
  TTGATACGAAGAGAGCAAGTGGAGCTGGAGGTCAGCACGTAAATACCACAGACAGTGCTGTTCGGATAGT
  TCATCTTCCCACAGGTATTATTTCTGAATGCCAGCAAGAAAGATCTCAGCTGAAGAACAGGGAGCTGGCT
  ATGAAAAAGTTACGTGCAAGGTTATACAGCATGCGTCTAGAGGAAGAAACTGCTAAAAGATACAGCGCTC
  GAAAGATCCAGGTTGGAACTAAAGGAAGATCAGAGAAAATAAGAACATACAACTTTCCACAAAATAGGGT
  CACAGATCACAGAATAAACAAATCACTACATGACCTTGAGAGCTTCATGCAAGGAGACTGTCTTCTGGAT
  GACCTGATACAGTCACTGAAGGACTATTCAGATTATGAATCTCTAGTAGAAATGATTTCCAGAAAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212532 representing NM_001025723
  Red=Cloning site Green=Tags(s)
  
  MRSGFLRSARRLWARRAISRMPPPSEELLARGGPLRAFLERRVGSEAGGLDAGSPQLAAAARLLNEKERE
  LRDTESLLHDENEDLKKLAESEIALCQKEIAELKHRIISLLVPSEDMDGSDLILEVTAGVGGQEAMLFTS
  EMFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGPEAYRHMKFEGGVHRVQRVPKTERQGRIHTS
  TMTVAILPQPTEIKLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGIISECQQERSQLKNRELA
  MKKLRARLYSMRLEEETAKRYSARKIQVGTKGRSEKIRTYNFPQNRVTDHRINKSLHDLESFMQGDCLLD
  DLIQSLKDYSDYESLVEMISRKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025723
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025723.1, NP_001020894.1
• RefSeq Size: 1264 bp
• RefSeq ORF: 1122 bp
• Locus ID: 361473
• UniProt ID: Q4V7E5
• Cytogenetics: 1q11
• MW: 42.3 kDa
• Gene Summary: Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.[UniProtKB/Swiss-Prot Function]