Tmem165 (NM_001024802) Rat Tagged ORF Clone

CAT#: RR212507

Tmem165 (Myc-DDK-tagged ORF) - Rat transmembrane protein 165 (Tmem165), (10 ug)

Product Images

Circular map for RR212507

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem165
• Synonyms: Tparl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212507 representing NM_001024802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCGGCCCGGGGGAGCGGCCGGGCGCCGACGCGACGGCTGCTCGTGCTCTTGCTGCTTCCGT
  TGCTCTGGGCCCCGGCTGGGGTCCGCGCTGGCCCCGAGGAAGACCTGAGCCACCGGAACCAGGAGCCGCC
  GGCGCCCGCCCAGCAGCTGCAGCCGCAGCCCGCGGCAGTACAGGGCCTCGAGCCGGCCCGGGCCGAGAAA
  GGATTTACACCGGCCGCCCCAGTTCATACCAACAGAGAAGATGCAGCTACCCAGGCAAATCTGGGATTTA
  TCCATGCGTTTGTTGCTGCCATATCAGTCATCATAGTGTCCGAACTCGGCGACAAGACATTTTTCATAGC
  TGCCATCATGGCGATGCGCTATAACCGGCTGACTGTGCTGGCTGGCGCCATGCTTGCTTTGGCCTTGATG
  ACGTGCTTGTCGGTTTTGTTTGGCTATGCCACCACAGTCATCCCCAGGGTGTACACATACTATGTCTCAA
  CTGCACTCTTTGCCATTTTTGGCATTAGAATGCTTCGGGAAGGTTTGAAGATGAGCCCAGACGAGGGTCA
  GGAGGAGCTCGAAGAAGTGCAAGCAGAGTTGAAGAAGAAAGATGAAGAATTCCAACGAACCAAACTCTTA
  AACGGGCCAGATGTTGAAACTGGTACAAGCACAGCAATACCTCAGAAAAAGTGGTTACACTTTATTTCAC
  CCATTTTTGTCCAAGCACTCACGTTAACATTCTTGGCGGAATGGGGTGATCGCTCTCAACTCACTACCAT
  TGTTCTGGCAGCCAGAGAGGATCCCTATGGTGTGGCAGTGGGTGGCACAGTGGGACACTGCCTGTGCACA
  GGATTGGCAGTAATTGGAGGAAGGATGATAGCACAAAAGATCTCTGTCAGAACTGTGACGATCATCGGAG
  GCATCGTGTTTCTGGCATTTGCATTTTCTGCTCTGTTTATAAGTCCAGAGTCTGGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212507 representing NM_001024802
  Red=Cloning site Green=Tags(s)
  
  MAASARGSGRAPTRRLLVLLLLPLLWAPAGVRAGPEEDLSHRNQEPPAPAQQLQPQPAAVQGLEPARAEK
  GFTPAAPVHTNREDAATQANLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALALM
  TCLSVLFGYATTVIPRVYTYYVSTALFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLL
  NGPDVETGTSTAIPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHCLCT
  GLAVIGGRMIAQKISVRTVTIIGGIVFLAFAFSALFISPESGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024802
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024802.1, NP_001019973.1
• RefSeq Size: 1897 bp
• RefSeq ORF: 972 bp
• Locus ID: 364137
• UniProt ID: Q4V899
• Cytogenetics: 14p11
• MW: 34.8 kDa
• Gene Summary: May function as a calcium/proton transporter involved in calcium and in lysosomal pH homeostasis. Therefore, it may play an indirect role in protein glycosylation (By similarity).[UniProtKB/Swiss-Prot Function]