Ccdc34 (NM_001108587) Rat Tagged ORF Clone

CAT#: RR212129

Ccdc34 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 34 (Ccdc34), (10 ug)

Product Images

Circular map for RR212129

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc34
• Synonyms: RGD1560583
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212129 representing NM_001108587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAAGGCCTAGTGCTGGCGCTGATTGGCCGGAGGGTGCGCGCGCGGAGACTGCTGGGAGAACGC
  GACGCCGGCCCTGCTCGGGCCGTTTGAACTTGGCGGGCCTGATGCAGGCGGCGGGTCCTTGGCGGGCCGC
  CTCTCCCGTTCCTTGCACCCGCTACCTCGCCCGCTGCAAGCCCGGCTTCCGGCCCTCCTCAAGCTTTGTC
  GTCGACCTCATAGTGGATTCTCAGGGCCCAGGGCTGGAGGATGTGGCGTTGACCGAGTGCTTGTTGCCGC
  CGTCTCTGAGCTGCAGCAACTCCACCCTGTCCCTGTTGTCTCCCCTTGGCCACCGGAGCTTTCCATTTGG
  CGAGGACGACAGTGAGGGGGAGGATGAAGAGGCCCTGGACGAAGATGCCTGTGAGTCAGAGGCCAAGGTG
  GAGAGCTTGGAAGGAATGGAGTTACAGGGCCGCTCCAGCTGTGAAGTTGAGTCCCAGGACAAACAAGAAG
  AGAAGCAAGTGCTGTTACAGGAAAGCAGTCTGACACCATGGGAGATGTGGTTTGTTGGCAAAGAAAAGGA
  AGAGCGTGGCCGGCTACAACAGAAAGTTCTGGAGGAATTAAATCGACAAATAGAAAAAAGAAAAGAAATG
  GAAGAACGTGAAAAAAGAAAGATAATTGCTGAAGTAAAGCACAAGGAATGGGTTCAGAAGAAAAATAAGC
  AGGAAAGGAAAGAAAGAGTACAAAAAATTAATAAAGAAATGGAAGAAAAAGCAGCAAAAAAACGGGAGAA
  AGAACATTTGCAAGAAAAAGCAAAAGAAAAATATCAAGAATGGTTGAAGAAAAAAAAAGCTGAAGAATGT
  GAGAAGAAGAAAAAAGAAAAGGTATTTTTAATTGAAGTAGCCAGTCATAGTACACACTTTGTTGTTTATA
  TGTGTATTTTAGAAACAAAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212129 representing NM_001108587
  Red=Cloning site Green=Tags(s)
  
  MTTRPSAGADWPEGARAETAGRTRRRPCSGRLNLAGLMQAAGPWRAASPVPCTRYLARCKPGFRPSSSFV
  VDLIVDSQGPGLEDVALTECLLPPSLSCSNSTLSLLSPLGHRSFPFGEDDSEGEDEEALDEDACESEAKV
  ESLEGMELQGRSSCEVESQDKQEEKQVLLQESSLTPWEMWFVGKEKEERGRLQQKVLEELNRQIEKRKEM
  EEREKRKIIAEVKHKEWVQKKNKQERKERVQKINKEMEEKAAKKREKEHLQEKAKEKYQEWLKKKKAEEC
  EKKKKEKVFLIEVASHSTHFVVYMCILETKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108587
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108587.1, NP_001102057.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 936 bp
• Locus ID: 362187
• Cytogenetics: 3q34
• MW: 35.7 kDa