Gpx3 (NM_022525) Rat Tagged ORF Clone

CAT#: RR211963

Gpx3 (Myc-DDK-tagged ORF) - Rat glutathione peroxidase 3 (Gpx3), (10 ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Product Images

Circular map for RR211963

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Symbol: Gpx3
• Synonyms: GPx-3; GPx-P; Gpxp; GSHPx-3; GSHPx-P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211963 representing NM_022525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGATCCTTCGGGCATCCTGCCTCCTGTCCCTGCTCCTGGCCGGGTTTGTTCCGCCGGGCCGGG
  GACAAGAGAAGTCCAAGACAGACTGCCATGGCGGTATGAGCGGTACCATCTACGAGTATGGAGCCCTTAC
  CATCGATGGGGAGGAATACATTCCTTTTAAGCAGTATGCTGGCAAATACATCCTCTTTGTCAACGTAGCC
  AGCTACTGAGGTCTGACAGACCAATACCTTGAACTGAATGCACTACAAGAAGAACTTGGCCCATTCGGCC
  TGGTCATTCTGGGCTTCCCTTGCAACCAATTTGGCAAACAGGAGCCAGGCGAGAACTCGGAGATCCTGCC
  TAGTCTCAAGTACGTTCGACCGGGTGGGGGCTTTGTGCCTAATTTCCAGCTCTTTGAGAAAGGAGACGTG
  AACGGGGAGAAAGAGCAGAAGTTCTACACTTTCCTGAAGAACTCCTGCCCTCCCACTGCGGAACTCCTGG
  GCTCACCTGGCCGCCTCTTTTGGGAACCCATGAAGATCCATGACATCCGCTGGAACTTTGAGAAGTTCCT
  GGTGGGGCCAGATGGCATACCAATTATGCGCTGGTACCACCGGACCACAGTCAGCAACGTCAAGATGGAC
  ATCCTGTCCTATATGAGGCGGCAGGCAGCCCTGGGGGCCAGAGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211963 representing NM_022525
  Red=Cloning site Green=Tags(s)
  
  MARILRASCLLSLLLAGFVPPGRGQEKSKTDCHGGMSGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVA
  SY*GLTDQYLELNALQEELGPFGLVILGFPCNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDV
  NGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPIMRWYHRTTVSNVKMD
  ILSYMRRQAALGARGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022525
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022525.4
• RefSeq Size: 1488 bp
• RefSeq ORF: 681 bp
• Locus ID: 64317
• UniProt ID: P23764
• Cytogenetics: 10q22
• MW: 25.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted and is predominantly expressed in rat kidney, which appears to be the major source of the enzyme in plasma. Dysregulation of this isozyme has been associated with obesity-related metabolic complications. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Aug 2016]