Eif2b1 (NM_172029) Rat Tagged ORF Clone

CAT#: RR211873

  • TrueORF®

Eif2b1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_172029" in other vectors (3)

Reconstitution Protocol

USD 330.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Other products for "Eif2b1"

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Eif2b1
Synonyms eIF-2a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR211873 representing NM_172029
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACGGTGAGTTAATCAAGTACTTTAAGTCCCAGATGAAAGGAGACCCTAACATGGCCTCGGCGG
TGGCTGCCATCCAGACCTTGCTGGAATTCTTGAAGAGAGATAAAGGAGAGACAATCCAGGGCCTGAGAGC
CCATCTCACCAAAGCCATCGAAACCCTCTGCGCTGTGGACTCCTCGGTGGCCGTGTCCTCGGGCGGGGAA
CTCTTCCTTCGCTTCATCAGCCTCACCTCCCTGGAGTACTCCGACTACTCCAAATGTAAAAAGATCATGA
TCGAGAGAGGAGAGCTTTTCCTGAGCAGAATATCCCTGTCAAGAACTAAGATCGCCAGTCTATGCCATGC
TTTTATCAAAGACGGGGCGAGAATCTTGACTCACGCCTACTCCAGAGTTGTCCTGAGGGTCCTGGAAGAA
GCCGTGGCAGCCAAGAAACGTTTCAGTGTGTATATCACGGAGTCACAGCCTGATTTATCTGGTAAGAAAA
TGGCCAAAGCCCTCTGCCACCTCAATGTCCCTGTCACTGTGGTGCTGGACGCTGCTGTTGGCTATATCAT
GGAGAAAGTAGACCTTGTCATCGTTGGTGCTGAAGGAGTGGTAGAGAATGGAGGAATTATTAACAAGATC
GGAACCAACCAGATGGCCGTGTGTGCCAAAGCCCAGAACAAGCCCTTCTATGTGGTTGCAGAAAGTTTCA
AGTTTGTACGGCTCTTTCCACTCAACCAGCAAGATGTCCCAGATAAGTTTAAGTACAAGGCAGACACCCT
GAAGTCTGTGCAGGCTGGACAGGACCTCAAAGAGGAACACCCATGGGTCGACTACACCTCCCCATCCCTC
ATCACACTGCTGTTTACGGACTTAGGTGTGTTGACACCATCCGCTGTAAGCGACGAGCTCATCAAGCTGT
ACCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR211873 representing NM_172029
Red=Cloning site Green=Tags(s)

MEDGELIKYFKSQMKGDPNMASAVAAIQTLLEFLKRDKGETIQGLRAHLTKAIETLCAVDSSVAVSSGGE
LFLRFISLTSLEYSDYSKCKKIMIERGELFLSRISLSRTKIASLCHAFIKDGARILTHAYSRVVLRVLEE
AVAAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKVDLVIVGAEGVVENGGIINKI
GTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKSVQAGQDLKEEHPWVDYTSPSL
ITLLFTDLGVLTPSAVSDELIKLYL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172029
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172029.2, NP_742026.1
RefSeq Size 1502 bp
RefSeq ORF 918 bp
Locus ID 64514
UniProt ID Q64270
Cytogenetics 12q15
MW 33.7 kDa
Gene Summary alpha subunit of eukaryotic peptide initiation factor 2B involved with mRNA translation into protein; may regulate protein synthesis when peptide chain initiation is inhibited [RGD, Feb 2006]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.