Eif2b1 (NM_172029) Rat Tagged ORF Clone
CAT#: RR211873
- TrueORF®
Eif2b1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_172029" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Eif2b1 |
Synonyms | eIF-2a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR211873 representing NM_172029
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGTGAGTTAATCAAGTACTTTAAGTCCCAGATGAAAGGAGACCCTAACATGGCCTCGGCGG TGGCTGCCATCCAGACCTTGCTGGAATTCTTGAAGAGAGATAAAGGAGAGACAATCCAGGGCCTGAGAGC CCATCTCACCAAAGCCATCGAAACCCTCTGCGCTGTGGACTCCTCGGTGGCCGTGTCCTCGGGCGGGGAA CTCTTCCTTCGCTTCATCAGCCTCACCTCCCTGGAGTACTCCGACTACTCCAAATGTAAAAAGATCATGA TCGAGAGAGGAGAGCTTTTCCTGAGCAGAATATCCCTGTCAAGAACTAAGATCGCCAGTCTATGCCATGC TTTTATCAAAGACGGGGCGAGAATCTTGACTCACGCCTACTCCAGAGTTGTCCTGAGGGTCCTGGAAGAA GCCGTGGCAGCCAAGAAACGTTTCAGTGTGTATATCACGGAGTCACAGCCTGATTTATCTGGTAAGAAAA TGGCCAAAGCCCTCTGCCACCTCAATGTCCCTGTCACTGTGGTGCTGGACGCTGCTGTTGGCTATATCAT GGAGAAAGTAGACCTTGTCATCGTTGGTGCTGAAGGAGTGGTAGAGAATGGAGGAATTATTAACAAGATC GGAACCAACCAGATGGCCGTGTGTGCCAAAGCCCAGAACAAGCCCTTCTATGTGGTTGCAGAAAGTTTCA AGTTTGTACGGCTCTTTCCACTCAACCAGCAAGATGTCCCAGATAAGTTTAAGTACAAGGCAGACACCCT GAAGTCTGTGCAGGCTGGACAGGACCTCAAAGAGGAACACCCATGGGTCGACTACACCTCCCCATCCCTC ATCACACTGCTGTTTACGGACTTAGGTGTGTTGACACCATCCGCTGTAAGCGACGAGCTCATCAAGCTGT ACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211873 representing NM_172029
Red=Cloning site Green=Tags(s) MEDGELIKYFKSQMKGDPNMASAVAAIQTLLEFLKRDKGETIQGLRAHLTKAIETLCAVDSSVAVSSGGE LFLRFISLTSLEYSDYSKCKKIMIERGELFLSRISLSRTKIASLCHAFIKDGARILTHAYSRVVLRVLEE AVAAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKVDLVIVGAEGVVENGGIINKI GTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKSVQAGQDLKEEHPWVDYTSPSL ITLLFTDLGVLTPSAVSDELIKLYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_172029 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172029.2, NP_742026.1 |
RefSeq Size | 1502 bp |
RefSeq ORF | 918 bp |
Locus ID | 64514 |
UniProt ID | Q64270 |
Cytogenetics | 12q15 |
MW | 33.7 kDa |
Gene Summary | alpha subunit of eukaryotic peptide initiation factor 2B involved with mRNA translation into protein; may regulate protein synthesis when peptide chain initiation is inhibited [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN211873 | Eif2b1 (untagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug) |
USD 330.00 |
|
RR211873L3 | Lenti ORF clone of Eif2b1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug) |
USD 630.00 |
|
RR211873L4 | Lenti ORF clone of Eif2b1 (mGFP-tagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 1 alpha (Eif2b1), (10 ug) |
USD 630.00 |
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