Hs3st6 (NM_001109450) Rat Tagged ORF Clone

CAT#: RR211570

Hs3st6 (Myc-DDK-tagged ORF) - Rat heparan sulfate (glucosamine) 3-O-sulfotransferase 6 (Hs3st6), (10 ug)

Product Images

Circular map for RR211570

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hs3st6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211570 representing NM_001109450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTAGCAGTGTCCTGGGCGGCGGGGCCGGGGACCTCCAGGGCGTAGGGACGGGCCAGGGGACTG
  CACTGCGTGCTCCCCGTGCTCTGCTGGCGCTCACAGCTCTGTTGCTAAGCGCCTACTGCCTCTTTGCCCT
  CCCCGGCCGCTGTCCACCTGCCGCCCGCGCCCCTGCTCCGGTTCCCGCACCAGCCGAGCCGCCCCAAGCC
  TCCCGCCGTCTGGGAGCGCCCGGCCTGCCGGTGGCCAGCGGCCCGGGGCGCCGGCGCTTCCCCCAGGCGC
  TCATCGTGGGCGTGAAGAAAGGCGGCACGCGCGCGTTGCTGGAGTTCCTGCGCTTGCACCCCGACGTCCG
  TGCACTTGGCTCTGAACCCCACTTCTTCGACAGGTGCTATGATCGCGGCCTCGCCTGGTACCGGGGTCTG
  ATGCCACGTACCCTGGATGGGCAGATCACCATGGAGAAGACCCCCAGCTACTTTGTGACTCAGGAGGCTC
  CCCGCCGAATCCATGATATGTCCCCAGACACCAAGCTGATTGTGGTGGTGCGGAACCCTGTGACCCGGGC
  CATCTCTGATTATGCACAGACACTCTCCAAGACCCCAGGTTTGCCCAGCTTCCGTGCCCTGGCCTTCCGC
  CACGGCCTGGGTCCCGTGGACACGGCCTGGAGCGCTGTACGCATTGGCCTCTACGCTCAGCACTTGGACA
  ACTGGCTACGCTACTTCCCTCTGTCCCACTTCCTGTTTGTAAGCGGTGAGCGCCTGGTCAGTGATCCAGC
  TGGCGAAGTGGGCCGTGTACAGGACTTTTTGGGTCTCAAACGGGTTGTCACTGACAAACACTTCTACTTC
  AATGCTACCAAGGGCTTCCCCTGCCTCAAAAAGGCCCAGGGTAGTGGGCGCCCCCGATGCTTGGGTAAAT
  CCAAGGGCCGGCCTCACCCCCGAGTGCCGGAGGCTGTGGTTCAGCGCCTGCAGGCTTTCTACCGGCCCTT
  CAACCGCAAGTTCTACCAGATGACTGGCCAGGACTTTGGCTGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211570 representing NM_001109450
  Red=Cloning site Green=Tags(s)
  
  MAGSSVLGGGAGDLQGVGTGQGTALRAPRALLALTALLLSAYCLFALPGRCPPAARAPAPVPAPAEPPQA
  SRRLGAPGLPVASGPGRRRFPQALIVGVKKGGTRALLEFLRLHPDVRALGSEPHFFDRCYDRGLAWYRGL
  MPRTLDGQITMEKTPSYFVTQEAPRRIHDMSPDTKLIVVVRNPVTRAISDYAQTLSKTPGLPSFRALAFR
  HGLGPVDTAWSAVRIGLYAQHLDNWLRYFPLSHFLFVSGERLVSDPAGEVGRVQDFLGLKRVVTDKHFYF
  NATKGFPCLKKAQGSGRPRCLGKSKGRPHPRVPEAVVQRLQAFYRPFNRKFYQMTGQDFGWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109450
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109450.1, NP_001102920.1
• RefSeq Size: 1231 bp
• RefSeq ORF: 1029 bp
• Locus ID: 684979
• Cytogenetics: 10q12
• MW: 37.5 kDa