Magec2 (NM_001025713) Rat Tagged ORF Clone

CAT#: RR211555

MGC114529 (Myc-DDK-tagged ORF) - Rat similar to melanoma antigen family A, 10 (MGC114529), (10 ug)

Product Images

Circular map for RR211555

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Magec2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211555 representing NM_001025713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCTACGGAAAGCACCCACGCCTCAGCCTGGAAGAAGGTGTAAACCTCCAGAATCCAACTGAAA
  CGGATGAGGTGATGTTCCCAGTAGTTCCCACACCTGAGGAAGAAGAGAGAGAGGGAGACGAGGAGGTCCA
  ACAGGAAGAGGAAGAAAATACCAATGAACAGGAAAATGAGGACGATAACAATGAGAAGGAAGAAGAGGAA
  GAAGAGGAGAAAGAAGAGGAAAAGGAAGAGTCTGATGACCAAGTGAATGAGGAAGAAGATGAGACTGGAG
  AAGAAGACTTAACTTCCATCTTTTTGTCATCTTCTAATGTCTCTTTAGCTACTTTCTCACCTCCATCTCC
  TTCCTCCTCCTCCTCCTGCTCCTCCTTCTCCTCTTCTTCTTCTTCTTCCTCCTCCTCTTCCCTCTTTTCT
  CTGTTTCAGAGCAACCTAGTGGAGAATGAGGGCTTTGCTGTCGGGATGCTGGGCATTTTTCAGAATGCTC
  ACAGCTTCTTTCCCTCACCTACTTTGGGGGGAAATTTAGATGAAGGAGTTGACTCCGAGGAAGAGAGTTC
  AGGTGTCATCTCGTCTCCAGAAGACCCTGGCTCCTTGCTCGATACTATAATACAAGAAAAGGCTACTGAT
  TTGGTGTTCCTTTTCATTTACAAGTATAGAATGAAGGAACCCATTACATTAAGTGAAATCCATGAGGTTG
  TCACTAAAGAGTATGAGAACCATTTTCCTGTCATTTTCACTGAAGCTTCTAAATGCTTGGAGATGACCTT
  TGGGATTGATATAAAGGAAAGTGATCTCCTAAGCAGTGCTTATGTTTTTGTCAACTCACTGAACCTAACC
  TATGAAGATGTGCTGGGTGACACTGATAGGCTGCCTAGAAATGCTTTCCTGATAGTTATTCTGGGTGTAA
  TATTCATAGAAGGGAACTGTGCTTCTGAAGAAAGAATTTGGGAGTTCTTGAAACTGGTAGGAGTGTATGA
  TGGAGAAGAGCACTTCATTTGTGGGGATCCTAGGGAGTTCCTCACCACACATCTAGTGCAGCAAAATTAC
  CTGGAATATCGGGAGGTGCCTAATACCCAGCCTCCATGCTTTGAGTTCCTGTGGGGTCCAAGAGCGTATG
  CTGAAACTACCAAGATGAAAGTTTTGGAATTCTTGGCAAAGATGAATGGGTGTGACCCATCTGATTTCTC
  AGTCTGGTATGAAGAGGCTTTGAGAGATGAGGAAGAAAGGGCCTGGGCCTTAAATAACTCTGCAAATAGA
  AGCCCAAATATGTGGTTTGTAGAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211555 representing NM_001025713
  Red=Cloning site Green=Tags(s)
  
  MSRYGKHPRLSLEEGVNLQNPTETDEVMFPVVPTPEEEEREGDEEVQQEEEENTNEQENEDDNNEKEEEE
  EEEKEEEKEESDDQVNEEEDETGEEDLTSIFLSSSNVSLATFSPPSPSSSSSCSSFSSSSSSSSSSSLFS
  LFQSNLVENEGFAVGMLGIFQNAHSFFPSPTLGGNLDEGVDSEEESSGVISSPEDPGSLLDTIIQEKATD
  LVFLFIYKYRMKEPITLSEIHEVVTKEYENHFPVIFTEASKCLEMTFGIDIKESDLLSSAYVFVNSLNLT
  YEDVLGDTDRLPRNAFLIVILGVIFIEGNCASEERIWEFLKLVGVYDGEEHFICGDPREFLTTHLVQQNY
  LEYREVPNTQPPCFEFLWGPRAYAETTKMKVLEFLAKMNGCDPSDFSVWYEEALRDEEERAWALNNSANR
  SPNMWFVER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025713
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025713.1, NP_001020884.1
• RefSeq Size: 1944 bp
• RefSeq ORF: 1290 bp
• Locus ID: 317590
• Cytogenetics: Xq36
• MW: 48.7 kDa