Slc34a2 (NM_053380) Rat Tagged ORF Clone

CAT#: RR211468

Slc34a2 (Myc-DDK-tagged ORF) - Rat solute carrier family 34 (sodium phosphate), member 2 (Slc34a2), (10 ug)

Product Images

Circular map for RR211468

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc34a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211468 representing NM_053380
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTTGGCCCGAGTTGGAAAATGCCCACCCCAACCCCAATAAATTCATCGAAGGGGCCTCAGGTC
  CACAGTCTAGCATACCAGACAAAGACAAGGGGACCAGCAAAACAAATGACAGCGGGACTCCTGTAGCCAA
  GATTGAACTTCTACCCTCATATTCCGCCCTGGTTCTCATAGAGGAGCCCCCTGAGGGGAACGACCCCTGG
  GACCTGCCTGAACTCCAAGACAATGGGATCAAATGGTCAGAGAGAGACTCCAAAGGGAAGATTCTCTGCA
  TTTTCCAGGGGATTGGGAAATTCATCCTTCTCCTGGGGTTTCTCTACTTGTTTGTGTGCTCCCTGGATGT
  ACTCAGTAGTGCCTTCCAGCTTGTTGGAGGAAAAATGGCCGGCCAGTTCTTCAGCAACAACTCCATTATG
  TCTAATCCTGTGGCTGGACTTGTGATAGGGGTGCTGGTGACAGTCATGGTGCAAAGCTCCAGCACGTCCT
  CGTCAATCATCGTCAGCATGGTTGCCTCCTCCTTGCTGTCAGTCCGCGCTGCCATCCCCATCATCATGGG
  TGCTAACATCGGGACCTCCATCACCAACACGATTGTGGCACTTATGCAGGCAGGAGACAGAAATGAGTTC
  AGAAGGGCATTTGCAGGAGCTACTGTCCATGACTTCTTCAACTGGCTCTCTGTGTTGGTGCTCTTACCTC
  TGGAGGCTGCCACCCATTACCTGGAGAAACTGACCAACCTTGTGTTGGAAACCTTCAGCTTCCAGAATGG
  AGAAGATGCCCCAGACATTCTGAAAGTCATAACAGACCCCTTCACAAAGCTCATCATCCAGCTGGATAAA
  AAAGTCATCCAACAAATTGCAATGGGTGACTCAGAAGCCCAGAACAAGAGCCTGATCAAGATCTGGTGTA
  AAACTATAAGCAATGTGATTGAGGAGAATGTCACTGTCCCCTCGCCTGATAACTGCACCTCTCCTTCATA
  TTGCTGGACTGATGGCATACAGACCTGGACCATCCAGAATGTGACCGAGAAGGAGAACATTGCTAAATGC
  CAGCACATCTTCGTGAATTTCAGCCTCCCAGACCTCGCTGTGGGCATCATCCTGCTCACTGTCTCCCTGC
  TGATCCTTTGCGGCTGTCTGATCATGATTGTCAAACTGTTGGGTTCTGTGCTCAGGGGACAGGTTGCCAC
  TGTCATCAAGAAGACCCTTAATACTGATTTCCCCTTTCCCTTTGCCTGGCTGACTGGCTACCTGGCCATC
  CTTGTGGGGGCAGGCATGACCTTCATAGTGCAAAGCAGCTCTGTGTTCACTTCTGCCATGACGCCACTCA
  TCGGTATTGGGGTGATCAGCATCGAGAGGGCATATCCACTCACGCTGGGCTCCAACATTGGCACCACCAC
  GACTGCCATTCTGGCTGCCTTGGCCAGCCCAGGCAACACATTGAGGAGTTCCCTCCAGATTGCCCTGTGC
  CATTTTTTCTTCAACATCTCTGGAATATTGCTGTGGTACCCAATCCCATTCACCCGTCTGCCCATCCGTC
  TGGCTAAAGGCCTGGGCAACATCTCTGCCAAGTATCGCTGGTTCGCAGTCTTCTATCTCATCTTCTTCTT
  CCTCCTGACCCCGCTGACAGTGTTTGGCCTGTCCCTGGCTGGCTGGCCAGTGCTGGTGGGCGTGGGTGTG
  CCCATCATTCTGCTGATCCTCCTGGTCCTGTGCCTCCGGATGCTGCAGGCCCGCTGCCCTCGCATCCTGC
  CCCTGAAGCTCCGGGACTGGAATTTCCTACCCTTGTGGATGCACTCTCTGAAACCCTGGGACAACATCAT
  CTCCCTGGCCACCTCCTGCTTCCAGAGGCGCTGTTGCTGCTGTTGCCGTGTGTGCTGCCGTGTCTGCTGC
  ATGGTGTGTGGCTGCAAGTGCTGCCGCTGCAGCAAGTGCTGCAAGAACCTGGAGGAGGAGGAAAAGGAGC
  AGGACGTCCCAGTCAAGGCTTCTGGGGGCTTTGACAATACAGCTATGAGCAAAGAATGCCAAGATGAGGG
  CAAGGGCCAAGTGGAAGTTCTGGGCATGAAGGCCCTGTCCAACACTACAGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211468 representing NM_053380
  Red=Cloning site Green=Tags(s)
  
  MAPWPELENAHPNPNKFIEGASGPQSSIPDKDKGTSKTNDSGTPVAKIELLPSYSALVLIEEPPEGNDPW
  DLPELQDNGIKWSERDSKGKILCIFQGIGKFILLLGFLYLFVCSLDVLSSAFQLVGGKMAGQFFSNNSIM
  SNPVAGLVIGVLVTVMVQSSSTSSSIIVSMVASSLLSVRAAIPIIMGANIGTSITNTIVALMQAGDRNEF
  RRAFAGATVHDFFNWLSVLVLLPLEAATHYLEKLTNLVLETFSFQNGEDAPDILKVITDPFTKLIIQLDK
  KVIQQIAMGDSEAQNKSLIKIWCKTISNVIEENVTVPSPDNCTSPSYCWTDGIQTWTIQNVTEKENIAKC
  QHIFVNFSLPDLAVGIILLTVSLLILCGCLIMIVKLLGSVLRGQVATVIKKTLNTDFPFPFAWLTGYLAI
  LVGAGMTFIVQSSSVFTSAMTPLIGIGVISIERAYPLTLGSNIGTTTTAILAALASPGNTLRSSLQIALC
  HFFFNISGILLWYPIPFTRLPIRLAKGLGNISAKYRWFAVFYLIFFFLLTPLTVFGLSLAGWPVLVGVGV
  PIILLILLVLCLRMLQARCPRILPLKLRDWNFLPLWMHSLKPWDNIISLATSCFQRRCCCCCRVCCRVCC
  MVCGCKCCRCSKCCKNLEEEEKEQDVPVKASGGFDNTAMSKECQDEGKGQVEVLGMKALSNTTVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053380
• ORF Size: 2085 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053380.2, NP_445832.1
• RefSeq Size: 3515 bp
• RefSeq ORF: 2088 bp
• Locus ID: 84395
• UniProt ID: Q9JJ09
• Cytogenetics: 14q11
• MW: 76.4 kDa
• Gene Summary: may act as a sodium dependent phosphate transporter; may provide inorganic phosphate for the synthesis of lung surfactant [RGD, Feb 2006]