Kcnab3 (NM_031652) Rat Tagged ORF Clone

CAT#: RR211462

Kcnab3 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, shaker-related subfamily, beta member 3 (Kcnab3), (10 ug)

Product Images

Circular map for RR211462

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnab3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211462 representing NM_031652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGTGTCTATCGCGTGTACCGAGCAGAACCTTCGCAGCCGGAGCAGTGAGGACCGTCTCTGTGGAC
  CCCGGCCGGGTCCTGGAGGCGGTAATGGAGGGCCAGTGGGCGGAGGGCATGGGAACCCTCCGGGGGGTGG
  AGGATTGGGCTCCAAGTCACGGACTGCAGTGGTCCCCCGACCCCCAGCACCTGCTGGGGCCCTTCGAGAG
  AGCACCGGCCGAGGCACTGGCATGAAATACAGGAACCTAGGAAAGTCTGGACTCCGGGTGTCCTGTCTTG
  GCCTCGGAACCTGGGTCACATTTGGCTCTCAGATCTCAGATGAGACAGCAGAAGATCTCCTGACAGTAGC
  CTATGAGCACGGTGTAAACCTGTTTGACACCGCTGAAGTGTACGCAGCAGGAAAGGCTGAAAGAACCCTA
  GGCAACATCCTCAAGAGCAAAGGTTGGAGGAGATCAAGCTATGTTATCACCACCAAGATTTTTTGGGGAG
  GACAGGCAGAAACTGAGCGAGGCTTGAGTCGCAAACACATCATTGAAGGCTTGCAAGGATCCCTGGACCG
  CCTCCAACTGGAATACGTTGACATAGTATTTGCCAATCGTTCAGACCCCAGCAGTCCTATGGAGGAGATT
  GTAAGAGCCATGACCTATGTCATCAACCAGGGCCTGGCCCTATACTGGGGGACATCCAGGTGGAGTGCTG
  CAGAAATCATGGAGGCCTACTCCATGGCCAGACAGTTCAATCTGATCCCTCCTGTGTGTGAACAAGCGGA
  GAACCACTTCTTTCAGAGGGAGAAGGTGGAGATGCAGCTGCCTGAGCTCTACCACAAAATTGGTGTTGGC
  TCAGTCACTTGGTCTCCCCTGGCGTGCAGCCTCATTACTAGCAAGTACGATGGGCAAGTTCCAGATGCTT
  GCAAGGCCACTGTCAAGGGCTACCAGTGGCTCAAAGAAAAAGTGCAAAGTGAAGATGGCAAGAAGCAACA
  AGCCAGAGTGACGGACCTTCTCCCCATCGCTCACCAGCTAGGCTGCACCGTGGCCCAACTTGCTATAGCC
  TGGTGTCTCCGAAGCGAAGGTGTCAGCTCAGTTTTACTGGGGGTGTCCAGTGCAGAGCAGCTGATGGAAC
  ATCTGGGGTCCCTACAGGTGCTGGGTCAGCTGACGCCGCAAACGGTGATGGAAATAGATGCGCTCTTGGG
  GAACAAATCGCATTCCAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211462 representing NM_031652
  Red=Cloning site Green=Tags(s)
  
  MQVSIACTEQNLRSRSSEDRLCGPRPGPGGGNGGPVGGGHGNPPGGGGLGSKSRTAVVPRPPAPAGALRE
  STGRGTGMKYRNLGKSGLRVSCLGLGTWVTFGSQISDETAEDLLTVAYEHGVNLFDTAEVYAAGKAERTL
  GNILKSKGWRRSSYVITTKIFWGGQAETERGLSRKHIIEGLQGSLDRLQLEYVDIVFANRSDPSSPMEEI
  VRAMTYVINQGLALYWGTSRWSAAEIMEAYSMARQFNLIPPVCEQAENHFFQREKVEMQLPELYHKIGVG
  SVTWSPLACSLITSKYDGQVPDACKATVKGYQWLKEKVQSEDGKKQQARVTDLLPIAHQLGCTVAQLAIA
  WCLRSEGVSSVLLGVSSAEQLMEHLGSLQVLGQLTPQTVMEIDALLGNKSHSKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031652
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031652.1, NP_113840.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1215 bp
• Locus ID: 58981
• UniProt ID: Q63494
• Cytogenetics: 10q24
• MW: 43.7 kDa
• Gene Summary: beta subunit of voltage gated potassium channel; involved in altering the activation kinetics of channel Kv1.4 [RGD, Feb 2006]