Cd2 (NM_012830) Rat Tagged ORF Clone

CAT#: RR210923

Cd2 (Myc-DDK-tagged ORF) - Rat Cd2 molecule (Cd2), (10 ug)

Product Images

Circular map for RR210923

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd2
• Synonyms: CD2R; LFA-2; LFA2; OX-34; OX34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210923 representing NM_012830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATGTAAATTCCTAGGGAGTTTCTTTCTGCTCTTCAGCCTGTCCAGCAAAGGAGCAGACTGCAGAG
  ACAGTGGGACCGTCTGGGGTGCCCTGGGTCATGGCATCAACCTGAACATCCCTAACTTTCAAATGACTGA
  TGATATTGATGAGGTGCGATGGGAGAGGGGGAGCACCCTGGTTGCCGAGTTTAAAAGGAAGATGAAGCCT
  TTTTTGAAATCGGGAGCATTTGAGATCTTAGCAAATGGAGACTTGAAGATAAAGAATCTGACAAGAGATG
  ACAGTGGCACCTATAATGTAACGGTATACAGCACAAATGGGACACGTATCCTGGACAAGGCACTGGACTT
  GAGGATTCTAGAGATGGTCTCAAAGCCGATGATCTACTGGGAGTGCAGCAACGCAACCCTGACCTGTGAG
  GTCTTGGAAGGAACAGATGTTGAACTAAAGCTGTACCAAGGAAAGGAGCATCTCAGGAGCCTCCGTCAGA
  AGACCATGAGTTACCAGTGGACCAACCTGAGAGCACCGTTTAAGTGCAAGGCGGTAAACAGGGTCAGCCA
  GGAGTCTGAGATGGAAGTTGTCAACTGTCCAGAGAAAGGTCTGCCCCTCTATCTCATAGTGGGGGTCAGT
  GCAGGAGGACTCCTCTTGGTGTTCTTTGGGGCGCTGTTTATTTTCTGTATCTGCAAGAGGAAAAAACGGA
  ACAGGAGGAGAAAAGGTGAAGAGCTGGAGATAAAAGCTTCCAGAATGAGCACTGTGGAAAGGGGACCCAA
  GCCACACTCAACCCAGGCCTCAGCACCAGCATCACAGAATCCAGTGGCTTCCCAAGCTCCTCCTCCACCT
  GGCCATCATCTCCAGACACCTGGCCATCGTCCCCTGCCTCCAAGCCACCGTAACCGTGAGCACCAGCCGA
  AGAAGAGACCTCCTCCATCAGGCACACAGGTTCACCAGCAGAAAGGCCCTCCTTTGCCCAGACCCCGAGT
  TCAGCCAAAGCCTCCCTGTGGGAGTGGAGATGTTTCGTTGCCACCCCCTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210923 representing NM_012830
  Red=Cloning site Green=Tags(s)
  
  MRCKFLGSFFLLFSLSSKGADCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKMKP
  FLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPMIYWECSNATLTCE
  VLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVSQESEMEVVNCPEKGLPLYLIVGVS
  AGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPP
  GHHLQTPGHRPLPPSHRNREHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012830
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012830.1, NP_036962.1
• RefSeq Size: 1168 bp
• RefSeq ORF: 1035 bp
• Locus ID: 497761
• UniProt ID: P08921
• Cytogenetics: 2q34
• MW: 38.4 kDa
• Gene Summary: cell surface antigen involved in T lymphocyte activation and proliferation; interacts with CD5 [RGD, Feb 2006]