Bdnf (NM_012513) Rat Tagged ORF Clone
Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)
|Product Name||Bdnf (NM_012513) Rat Tagged ORF Clone|
|Type||Rat Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RR210837 representing NM_012513
Red=Cloning site Blue=ORF Green=Tags(s)
>RR210837 representing NM_012513
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||771 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||4252 bp|
|RefSeq ORF||774 bp|
|Gene Summary||plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]|
|The use of this cDNA Clones has been cited in the following citations:|
Noninvasive, targeted gene therapy for acute spinal cord injury using LIFU-mediated BDNF-loaded cationic nanobubble destruction
,Song, Z;Ye, Y;Zhang, Z;Shen, J;Hu, Z;Wang, Z;Zheng, J;,
Biochem. Biophys. Res. Commun. 2018
,PubMed ID 29360450
|cDNA Clone Resources|
|RN210837||Bdnf (untagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)||
|RR210837L1||Lenti ORF clone of Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)||
|RR210837L2||Lenti ORF clone of Bdnf (mGFP-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)||
|RR210837L3||Lenti ORF clone of Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)||
|RR210837L4||Lenti ORF clone of Bdnf (mGFP-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)||