Fnta (NM_012847) Rat Tagged ORF Clone

CAT#: RR210495

Fnta (Myc-DDK-tagged ORF) - Rat farnesyltransferase, CAAX box, alpha (Fnta), (10 ug)

Product Images

Circular map for RR210495

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fnta
• Synonyms: PFAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210495 representing NM_012847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACTGAGGGGGTCGGGGAATCTGCGCCAGGCGGTGAGCCGGGACAGCCAGAGCAGCCGCCGC
  CCCCGCCTCCTCCGCCGCCAGCACAGCAGCCGCAGGAAGAAGAGATGGCGGCCGAGGCCGGGGAAGCAGC
  GGCGTCCCCTATGGACGACGGGTTTCTGAGCCTGGACTCGCCCACCTATGTCTTGTACAGGGACAGGGCA
  GAGTGGGCTGACATAGACCCAGTGCCCCAGAATGATGGCCCCAGTCCAGTGGTCCAGATCATCTACAGTG
  AAAAGTTTAGAGACGTCTATGATTACTTCCGAGCTGTTCTGCAGCGCGATGAAAGAAGCGAACGAGCCTT
  TAAGCTCACTCGAGATGCTATTGAGTTAAACGCAGCCAACTATACGGTGTGGCATTTTCGGAGAGTTCTC
  TTGAGGTCGCTTCAGAAGGATCTGCAAGAAGAAATGAACTACATCATCGCAATAATTGAGGAACAGCCCA
  AAAACTATCAAGTTTGGCACCATAGGAGAGTATTAGTGGAGTGGCTGAAAGATCCTTCTCAAGAGCTCGA
  GTTCATCGCCGATATCCTTAATCAGGATGCAAAGAATTACCATGCCTGGCAGCATCGACAGTGGGTCATT
  CAGGAGTTTCGACTTTGGGATAATGAGCTGCAGTATGTGGACCAGCTTCTCAAAGAGGATGTGAGAAATA
  ACTCTGTGTGGAACCAAAGACACTTCGTCATTTCTAATACCACTGGCTACAGTGATCGCGCTGTGTTGGA
  GAGAGAAGTCCAATATACTCTGGAAATGATCAAATTAGTGCCACACAATGAGAGTGCGTGGAACTACTTG
  AAAGGGATTTTGCAGGACCGTGGTCTTTCCAGATACCCTAATCTATTAAACCAGTTGCTTGATTTACAAC
  CAAGTCACAGCTCCCCCTACCTAATTGCCTTTCTTGTGGATATCTATGAAGACATGCTGGAAAACCAGTG
  TGACAACAAGGAGGACATTCTTAATAAAGCACTAGAGTTATGTGAGATTCTAGCTAAAGAAAAGGACACT
  ATAAGAAAGGAATATTGGAGATATATTGGACGGTCCCTCCAGAGTAAACACAGCAGAGAAAGTGACATAC
  CGGCGAGTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210495 representing NM_012847
  Red=Cloning site Green=Tags(s)
  
  MAATEGVGESAPGGEPGQPEQPPPPPPPPPAQQPQEEEMAAEAGEAAASPMDDGFLSLDSPTYVLYRDRA
  EWADIDPVPQNDGPSPVVQIIYSEKFRDVYDYFRAVLQRDERSERAFKLTRDAIELNAANYTVWHFRRVL
  LRSLQKDLQEEMNYIIAIIEEQPKNYQVWHHRRVLVEWLKDPSQELEFIADILNQDAKNYHAWQHRQWVI
  QEFRLWDNELQYVDQLLKEDVRNNSVWNQRHFVISNTTGYSDRAVLEREVQYTLEMIKLVPHNESAWNYL
  KGILQDRGLSRYPNLLNQLLDLQPSHSSPYLIAFLVDIYEDMLENQCDNKEDILNKALELCEILAKEKDT
  IRKEYWRYIGRSLQSKHSRESDIPASV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012847
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012847.1, NP_036979.1
• RefSeq Size: 1680 bp
• RefSeq ORF: 1134 bp
• Locus ID: 25318
• UniProt ID: Q04631
• Cytogenetics: 16q12.4
• MW: 44 kDa
• Gene Summary: subunit of the heterodimeric p21ras protein farnesyltransferase that attaches farnesyl residues to a cysteine at the fourth position from the C-terminus; may function as farnesyl pyrophosphate carrier [RGD, Feb 2006]