Cd5 (NM_019295) Rat Tagged ORF Clone

CAT#: RR210425

Cd5 (Myc-DDK-tagged ORF) - Rat Cd5 molecule (Cd5), (10 ug)

Product Images

Circular map for RR210425

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd5
• Synonyms: MGC114334
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210425 representing NM_019295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTCACGAAGTGCTGCTGGCTGCCACATACCTGCTGGGAACACTGGCTGCTTTCTGCCTTGGAC
  AGTCTGGAAGGGGCTTCGTAGGTGCCCAGGTGATGCTAAGTGGCTCCAACTCCAAGTGTCAGGGTCTAGT
  GGAGGTCCAGATGAATGGAATGAAAACGGTGTGCAGTTCCAGTTGGAGGCTGAGCCAGGATCTCTGGAAA
  AACGCCAATGAGGCCTCTACAGTGTGCCAGCAGCTGGGATGTGGTAACCCTCTGGCCCTTGGCCATTTGA
  CCTTATGGAACAGACCCAAGAACCAGATCCTCTGCCAAGGACCCCCGTGGTCTTTCTCCAACTGCAGCAC
  AAGTTCGCTGGGCCAGTGCCTTCCGCTGAGCCTGGTCTGCCTAGAGCCCCAGAAGACGACACCTCTACCC
  ACAACCACCCTACCCACCACCATGCCGGAGCCCACAGCCCCTCCCAGATTGCAGCTGGTGCCAGGACACG
  AGGGCCTGAGGTGCACAGGTGTGGTGGAATTCTACAATGGCAGCCGGGGTGGCACCATCCTCTATAAGGC
  CAAGGCCAGGCCCGTGGACCTGGGGAACCTCATCTGTAAGTCTCTGCAGTGTGGCTCTTTCCTGACACAT
  CTGTCCCGGATAGAGACAGCAGGAACTCCAGCTCCTGCAGAGCTGAGGGACCCCAGGCCCTTGCCAATTC
  GATGGGAGGCCCAGAACGGGAGCTGCACCTCACTACAGCAATGCTTCCAGAAAACAACGGTCCAGGAGGG
  CAGCCAGGCACTCGCAGTCGTCTGCTCTGATTTCCAGCCCAAGGTTCAGAGCCGCCTGGTGGGGGGCAGC
  AGCGTGTGTGAGGGCATCGCTGAAGTTCGCCAGAGGTCACAGTGGGCTGCCCTGTGTGACAGTTCTGCAG
  CCAGGGGTCCAGGACGGTGGGAGGAGCTATGCCAAGAGCAGCAGTGTGGCAACCTCATCTCCTTCCATGT
  GATGGATGCTGACAGGACCTCCCCGGGGGTCCTCTGTACCCAGGAGAAGCTGTCTCAGTGTTACCAGCTT
  CAGAAAAAAACCCACTGCAAGAGGGTATTCATCACATGCAAAGACCCAAACCCAGTGGGCCTGGCCCCAG
  GCACTGTGGCAAGCATCATCCTGACCCTGGTACTCCTGGTGGTGCTGATGGTCATGTGCGGTCCTCTGAT
  CTACAAGAAGCTGGTGAAGAAATTTCGTCAGAAGAAGCAGCGTCAGTGGATTGGTCCCACAGGAGTGAAC
  CAGAGCATGTCTTTCCATCGAAGCCACACGGCAACTGTGCGGTCCCAGGTTGAGAACCCGGCAGCCTCTC
  ACGTGGACAATGAGTACAGCCAGCCTCCCAGGAACTCTCGCCTGTCGGCTTATCCAGCTCTGGAAGGGGC
  CCTACACCGCTCTTCCACACAACCTGACAATTCCTCTGACAGTGACTATGACCTGCAAGTGGCTCAGAGA
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210425 representing NM_019295
  Red=Cloning site Green=Tags(s)
  
  MDSHEVLLAATYLLGTLAAFCLGQSGRGFVGAQVMLSGSNSKCQGLVEVQMNGMKTVCSSSWRLSQDLWK
  NANEASTVCQQLGCGNPLALGHLTLWNRPKNQILCQGPPWSFSNCSTSSLGQCLPLSLVCLEPQKTTPLP
  TTTLPTTMPEPTAPPRLQLVPGHEGLRCTGVVEFYNGSRGGTILYKAKARPVDLGNLICKSLQCGSFLTH
  LSRIETAGTPAPAELRDPRPLPIRWEAQNGSCTSLQQCFQKTTVQEGSQALAVVCSDFQPKVQSRLVGGS
  SVCEGIAEVRQRSQWAALCDSSAARGPGRWEELCQEQQCGNLISFHVMDADRTSPGVLCTQEKLSQCYQL
  QKKTHCKRVFITCKDPNPVGLAPGTVASIILTLVLLVVLMVMCGPLIYKKLVKKFRQKKQRQWIGPTGVN
  QSMSFHRSHTATVRSQVENPAASHVDNEYSQPPRNSRLSAYPALEGALHRSSTQPDNSSDSDYDLQVAQR
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019295
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019295.1, NP_062168.1
• RefSeq Size: 2059 bp
• RefSeq ORF: 1476 bp
• Locus ID: 54236
• UniProt ID: P51882
• Cytogenetics: 1q43
• MW: 53.4 kDa
• Gene Summary: binds CD2 glycoprotein on thymocytes; may play a role in T lymphocyte signal transduction [RGD, Feb 2006]