Cavin3 (NM_134449) Rat Tagged ORF Clone

CAT#: RR210216

Prkcdbp (Myc-DDK-tagged ORF) - Rat protein kinase C, delta binding protein (Prkcdbp), (10 ug)

Product Images

Circular map for RR210216

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cavin3
• Synonyms: DIG-2; Prkcdbp; Srbc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210216 representing NM_134449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGAGCGCACTGGAGCCCGGGCCTGTGCCCGGGGCGCCGGCTGGGGGTCCGGTGCACGCCGTCA
  CCGTGGTGACTTTGCTGGAGAAGCTGGCCACCATGCTAGAGGCGCTGCGGGAGAGGCAGGGGGGCCTGGC
  TGAGAGGCAGGGCGGCCTGGCGGGCTCGGTGCGCCGCATCCAGAGTGGCCTGGGCGCGCTGAGTCGCAGC
  CACGACACCACCAGCAACACACTGGCGCAGCTGCTGGCCAAGGCGGAGCGCGTGGGCTCCCACGCCGACG
  CAGCCCAGGAGCGGGCAGTGCACCGCGCCGCTCAGGTGCAGCGACTGGAGGCCAACCACGGGTTGCTGGT
  GGCGCGCGGGAAGCTGCACGTCCTGCTCTTCAAGGAGGAGACTGAAATTCCAGCCCGCGCCTTCCAGAAA
  GCACCAGAGCTCTTGGGCCCGGAGGACCAGTTGGTGCTAGGCCCAGAGCAGCCAGAGGATGAAGTTGGAG
  AGAGTTCTGATGAGGAACCCGTGGAGTCCCGGGCTCAGCGGCTGCGACGCACTGGCTTACAGAAGGTTCA
  AAGCCTGAAAAGGGCTTTTTCCAGTCGTAAAGGCTCTGAAGCAGCACAGCCCACGCCAGTCAAGCCGCCA
  CGCCTAGGTCCTGTCCGGAACTCCGAAGGCCCGGCAGAAGGCCAGCCTGCAGCTCAGCCTGCAATGGAGC
  CTGTGCTCCCGTCTGCCCTGGAGCCAGAACCTCCTCAGCCTACCAAGGAAGATCCTGAGAGACCTGTGCT
  TCAAATAGAGAGCGCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210216 representing NM_134449
  Red=Cloning site Green=Tags(s)
  
  MGESALEPGPVPGAPAGGPVHAVTVVTLLEKLATMLEALRERQGGLAERQGGLAGSVRRIQSGLGALSRS
  HDTTSNTLAQLLAKAERVGSHADAAQERAVHRAAQVQRLEANHGLLVARGKLHVLLFKEETEIPARAFQK
  APELLGPEDQLVLGPEQPEDEVGESSDEEPVESRAQRLRRTGLQKVQSLKRAFSSRKGSEAAQPTPVKPP
  RLGPVRNSEGPAEGQPAAQPAMEPVLPSALEPEPPQPTKEDPERPVLQIESAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134449
• ORF Size: 789 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134449.1, NP_604444.1
• RefSeq Size: 1049 bp
• RefSeq ORF: 792 bp
• Locus ID: 85332
• UniProt ID: Q9Z1H9
• Cytogenetics: 1q32
• MW: 27.9 kDa
• Gene Summary: bound and phosphorylated by protein kinase C delta; may be involved in PKC mediated control of cell growth; contains a leucine zipper-like motif and two PEST sequences [RGD, Feb 2006]