Pogk (NM_001107194) Rat Tagged ORF Clone

CAT#: RR210165

Pogk (Myc-DDK-tagged ORF) - Rat pogo transposable element with KRAB domain (Pogk), (10 ug)

Product Images

Circular map for RR210165

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pogk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210165 representing NM_001107194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGTCACAACCCTCGTCGCCGCTTGCGGCGACGCCACCCGCTCGGCCGGGAAATGGAGTCCCGAG
  CCTACCCTCTAAATTTGACCCTGAAAGAAGAGCAAAAAGAAGATGAGGTTGAGATCCAGGAATTGGAGGA
  TGGTCCCGTAGAGATGCAGAAAGTGCAAATCTGCTCGGAAGGCACGTGGGTACCAGCCCTATTTGATGAG
  GTGGCCATATATTTTTCGGATGAGGAGTGGGAAGTTCTGACAGAGCAACAAAAAGCCCTCTACCGGGAAG
  TCATGAGGATGAATTATGAGACTGTCCTGTCCCTGGAATTCCCATTCCCCAAGCCAGATATGATCAATCG
  GTTGGAAAGGGAAGAGGAGTGTCCTGAATCTGATGAGTGGCGGCTCCAGGGAGTAACCTTTGCAGAAAAT
  GAAGAATCTGATTTTAGGACTCCAGATTGGGCCAACCCGATGAATGCCACCTCCCATTTTCCTCAACCTC
  AGCACTTCAACAGCTTTGGGCTGCGTCTGCCGCAGGACATCACTGAACTGCCTGAGTGGAGTGAGGGGTA
  CCCCTTCTATATGGCCATGGGCTTCCCTGGGTATGACCTCTCAGCTGACGACTTGGCGAGCAAGTTTCAG
  TTCAGTCGCGGCATGCGCCGCAGTTACGATGCGGGGTTCAAGCTGATGGTGGTGGAGCACGCTGAGAGTA
  CCAACAACTGTCAGGCTGCCAAGCAGTTCGGGGTGTTGGAAAAGAATGTCCGAGACTGGCGCAAAGTGAA
  GCCACAGCTCCAAAACGCCCATGCCATGCGGCGGGCATTCCGAGGCCCCAAGAATGGAAGATTTGCTCTG
  GTAGACCAGCGGGTGGCTGAGTATGTCAGATACATGCAGGCCAAAGGGGACCCCATCACCAGGGAGGCAA
  TGCAGTTGAAAGCTCTTGAAATCGCCCAGGAAATGAACATTCCAGAGAAAGGGTTCAAGGCAAGTCTGGG
  CTGGTGTCGGAGGATGATGAGAAGGTATGACCTATCCCTGAGGCATAAAGTGCCAGTTCCTCAGCACCTG
  GCCGAGGACCTGACTGAGAAGCTCGTCACTTACCAGCAGAGTGTGCTTGCTCTGCGCCGGACACACGACT
  ATGAGGTGGCACAGATGGGCAATGCAGATGAAACACCCATCTGCTTAGAAGTGCCCTCTAGAGTGACTGT
  TGACAACCAGGGTGAAAAGCCCATCCTGGTCAAGACACCAGGCAGGGAGAAACTGAGGATCACAGCCATG
  CTGGGGGTCTTGGCTGATGGGAGGAAGTTACCACCATACATCATCTTGAGGGGGACATATATCCCCCCTG
  GGAAGTTTCCCAGTGGCATGGAAATCCGCTGCCACCGCTATGGATGGATGACTGAGGACTTGATGCAAGA
  CTGGTTGGAAGTTGTGTGGAGACGGAGAACTGGAGCTGTACCCAAACAACGAGGGATGCTGATCCTGAAT
  GGCTTCCGGTGCCATGCCACCGACTCTGTGAAGACTTCCATGGAGAGTATGAACACAGACATGGTGATTA
  TCCCGGGGGGCCTGACCTCCCAGCTGCAGGTGCTGGATGTGGTTGTCTACAAGCCACTGAATGACAGTGT
  TCGGGCCCAGTATTCCAACTGGCTTCTGGCTGGGAACCTGGCACTGAGCCCCACTGGAAATGCTAAGAAG
  CCACCGCTGGGCCTCTTCCTGGAGTGGATCATGGTTGCGTGGAATAGTATCTCAAGTGAATCAATTGTCC
  AGGGGTTCAGAAAGTGCCACATCTCTAGCAACTTAGAGGAAGAAGGTGATGTCCTCTGGGAAATCGAGGG
  CGAGTTGCCCAAAGAACGTGGTCATGAAGGTGTGGCTGAGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210165 representing NM_001107194
  Red=Cloning site Green=Tags(s)
  
  MPGHNPRRRLRRRHPLGREMESRAYPLNLTLKEEQKEDEVEIQELEDGPVEMQKVQICSEGTWVPALFDE
  VAIYFSDEEWEVLTEQQKALYREVMRMNYETVLSLEFPFPKPDMINRLEREEECPESDEWRLQGVTFAEN
  EESDFRTPDWANPMNATSHFPQPQHFNSFGLRLPQDITELPEWSEGYPFYMAMGFPGYDLSADDLASKFQ
  FSRGMRRSYDAGFKLMVVEHAESTNNCQAAKQFGVLEKNVRDWRKVKPQLQNAHAMRRAFRGPKNGRFAL
  VDQRVAEYVRYMQAKGDPITREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHL
  AEDLTEKLVTYQQSVLALRRTHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPILVKTPGREKLRITAM
  LGVLADGRKLPPYIILRGTYIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPKQRGMLILN
  GFRCHATDSVKTSMESMNTDMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTGNAKK
  PPLGLFLEWIMVAWNSISSESIVQGFRKCHISSNLEEEGDVLWEIEGELPKERGHEGVAEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107194
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107194.2, NP_001100664.2
• RefSeq Size: 6927 bp
• RefSeq ORF: 1869 bp
• Locus ID: 304941
• Cytogenetics: 13q23
• MW: 71.7 kDa