Klhdc10 (NM_001017456) Rat Tagged ORF Clone

CAT#: RR209937

Klhdc10 (Myc-DDK-tagged ORF) - Rat kelch domain containing 10 (Klhdc10), (10 ug)

Product Images

Circular map for RR209937

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klhdc10
• Synonyms: RGD1309792
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209937 representing NM_001017456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCTGCCCAGGGCTGGGACCGGAACCGCCGGCGGGGAGGAGGCGCAGCCGGCGGGGCCAGCGGGG
  TTAGCGGGGCCGGCGCGGCCGGCGGGGGTCGGGGCACCGGACAGCTCAACCGATTCGTGCAGCTCTCCGG
  ACGTCCGCACCTGCCAGGCCACAGACCTCCCCCAGCCCGCAGTGGACACCGTTGTGTGGCAGATAACACC
  AATCTCTATGTGTTTGGAGGGTATAACCCAGACTATGATGAATCGGGAGGGCCAGATAACGAAGACTATC
  CTCTCTTCAGGGAGCTTTGGAGGTATCATTTTGCAACAGGCGTGTGGCACCAGATGGGCACAGATGGCTA
  CATGCCCCGGGAATTGGCATCTATGTCACTTGTGCTGCATGGAAACAACCTGCTTGTCTTTGGAGGCACA
  GGCATCCCATTTGGGGAGAGCAACGGCAATGATGTCCACGTCTGCAATGTGAAGTACAAGAGATGGGCCT
  TACTCAGCTGTCGTGGGAAGAGACCCAGTCGGATATACGGACAGGCAATGGCCCTCATCAATGGGTCCCT
  TTACGTCTTTGGGGGAACAACTGGCTACATCTATAGCACAGACCTGCACAAGTTAGATCTCAACACCATG
  GTGTGGACACAGCTCAAACCAAACAACCTGTCCTGTGACCTGCCGGAGGAGAGGTACAGACATGAAATTG
  CTCATGATGGGCAGAGGATTTACATCTTGGGAGGAGGCACTTCCTGGACAGCCTATTCCTTAAACAAGAT
  CCACGCATATAACCTTGAAACAAATGCATGGGAGGAGATCGCAACAAAACCTCATGAAAAAATAGGTTTT
  CCTGCAGCCCGACGATGTCACAGTTGTGTTCAAATAAAAAATGATGTCTTCATTTGTGGTGGCTATAATG
  GAGAAGTGATCCTGGGAGACATCTGGAAGCTGAATCTTCAGACTTTTCAGTGGGTGAAGCTCCCCGCCAC
  CATGCCAGAGCCCGTTTACTTTCACTGTGCAGCTGTCACACCTGCCGGCTGCATGTATATCCACGGAGGA
  GTGGTGAACATCCACGAAAACAAACGGACTGGATCGCTGTTTAAGATCTGGCTGGTGGTACCTAGCCTGC
  TGGAACTGGCATGGGAGAAGCTGCTCGCGGCCTTCCCCAACCTAGCAAACCTTTCCCGAACACAGCTTCT
  GCACCTTGGACTCACACAGGAACTCATCGAGCGTTTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209937 representing NM_001017456
  Red=Cloning site Green=Tags(s)
  
  MSAAQGWDRNRRRGGGAAGGASGVSGAGAAGGGRGTGQLNRFVQLSGRPHLPGHRPPPARSGHRCVADNT
  NLYVFGGYNPDYDESGGPDNEDYPLFRELWRYHFATGVWHQMGTDGYMPRELASMSLVLHGNNLLVFGGT
  GIPFGESNGNDVHVCNVKYKRWALLSCRGKRPSRIYGQAMALINGSLYVFGGTTGYIYSTDLHKLDLNTM
  VWTQLKPNNLSCDLPEERYRHEIAHDGQRIYILGGGTSWTAYSLNKIHAYNLETNAWEEIATKPHEKIGF
  PAARRCHSCVQIKNDVFICGGYNGEVILGDIWKLNLQTFQWVKLPATMPEPVYFHCAAVTPAGCMYIHGG
  VVNIHENKRTGSLFKIWLVVPSLLELAWEKLLAAFPNLANLSRTQLLHLGLTQELIERLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017456
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017456.1, NP_001017456.1
• RefSeq Size: 1480 bp
• RefSeq ORF: 1233 bp
• Locus ID: 312199
• UniProt ID: Q5U3Y0
• Cytogenetics: 4q22
• MW: 45.4 kDa
• Gene Summary: Participates in the oxidative stress-induced cell death through MAP3K5 activation. Inhibits PPP5C phosphatase activity on MAP3K5 (By similarity).[UniProtKB/Swiss-Prot Function]