Cwc25 (NM_001108295) Rat Tagged ORF Clone

CAT#: RR209906

Cwc25 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 49 (Ccdc49), (10 ug)

Product Images

Circular map for RR209906

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cwc25
• Synonyms: Ccdc49; RGD1305258
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209906 representing NM_001108295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGAGGCGGAGACCTGAACCTGAAGAAGAGCTGGCACCCGCAGACCCTTCGCAATGTGGAGAAAGTGT
  GGAAGGCGGAGCAGAAACATGAAGCGGAGAGGAAAAAGATTGAGGAGTTGCAGCGAGAGCTTCGAGAGGA
  GCGAGCTCGGGAGGAGATGCAGCGCTACGCTGAGGATGTCGGGGCTGTCAAGAAAAAAGAAGAAAAGTTG
  GATTGGATGTACCAAGGCCCAGGTGGCATGGTGAGCCGCGATGAGTACCTGCTGGGGCGCCCCATTGACA
  AGTATGTTTTTGAGAAGATGGAGGAGAGGGAGGCCGGCTGCTCTTCTGAGACAGGACTCCTCCCAGGCTC
  AATCTTCGCCCCGTCGGGTGCTAATTCTCTTCTTGACATGGCCAGCAAAATCCGGGAAGACCCTCTCTTC
  ATCATAAGGAAGAAAGAGGAGGAGAAAAAAAGAGAAGTACTGAACAACCCTGTGAAAATGAGGAAAATCA
  AAGAGCTGTTGCAAATGAGTCTTGAAAAAAAAGAGAAGAAGAAGAAGAAGGAGAAGAAGAAGAAGCACAG
  GAAACACAAACACAGAAGCTCAAGCAGCGATCGCTCCAGCAGCGAGGATGAGCAGAGCCATGGCCGATCT
  CAAAAGAAGATGGCAAATTCCTTTCCTGTTTTGTCCAAAGTCCCTGGATATGGCTTACAGGTGAGGGACT
  CTGACCGGAACCAGGGGCTGCAGGGGTCTCTGGGTGAACAAAGGGCAGTAAAGAACCATTCCCGGTCAAG
  CTCCTCCCCCGCCAGGCATGCCAGCAGGAAAAGCACCAAGGAAGAGAGATCCCGGGACAGGAGGTCTCGA
  TCCCCCGGCAGAAAGTCACTGTCCCCAAGGCCCAGCAAACAGCACACCTCTAAGGCAAACAGGAAGGAGA
  GAGACAGCCCGTCACCTAAAAAGGAGGTCTACCAGCGGCGGCATGCTTCTGGGTACACCAGAAAACTCTC
  AGCGGAGGAACTAGAGCGGAAACGGCAGGAGATGATGGAAAATGCCAAGTGGAGGGAGGAAGAGAGGCTC
  AACACCCTCAAGAGGCACGCCAAGGAAGATGAGCAGGAGCACAGGCTGGAGAGGTTGGACTCTCGGTCCG
  GCAAGTTCATCCACCGCATGAAGCTGGAGAGCGCGTCCACCTCTTCCCTGGAGGACCGGGTCAAGCGCAA
  CATCCATTCTCTGCAGCGGACATCGATGGCTCTGGAGAAGAACTTCATGAGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209906 representing NM_001108295
  Red=Cloning site Green=Tags(s)
  
  MGGGDLNLKKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEEKL
  DWMYQGPGGMVSRDEYLLGRPIDKYVFEKMEEREAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLF
  IIRKKEEEKKREVLNNPVKMRKIKELLQMSLEKKEKKKKKEKKKKHRKHKHRSSSSDRSSSEDEQSHGRS
  QKKMANSFPVLSKVPGYGLQVRDSDRNQGLQGSLGEQRAVKNHSRSSSSPARHASRKSTKEERSRDRRSR
  SPGRKSLSPRPSKQHTSKANRKERDSPSPKKEVYQRRHASGYTRKLSAEELERKRQEMMENAKWREEERL
  NTLKRHAKEDEQEHRLERLDSRSGKFIHRMKLESASTSSLEDRVKRNIHSLQRTSMALEKNFMRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108295
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108295.1, NP_001101765.1
• RefSeq Size: 3195 bp
• RefSeq ORF: 1248 bp
• Locus ID: 360613
• Cytogenetics: 10q31
• MW: 48.7 kDa