Rhov (NM_138542) Rat Tagged ORF Clone

CAT#: RR209798

Rhov (Myc-DDK-tagged ORF) - Rat ras homolog gene family, member V (Rhov), (10 ug)

Product Images

Circular map for RR209798

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rhov
• Synonyms: Arhv; Chp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209798 representing NM_138542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCAGCGGGCGTGCCCAGCTTGGACAGATTGTCAGCGTCCGGCTGCGGAACCTGCCTCCCTGAGC
  GCCGCGGAGCCTGGTCCGGGCTGGCCCCTCTGCTACCCCGGGAGCGGGCCATGCCGCCGCGGGAGCTGAG
  CGAGGCCGAGCCACCGCCTCTCCCGGCCTCGACCCCTCCTCCGCGGCGGCGCAGCGCCCCTCCGGAGCTG
  GGCATCAAATGCGTGCTGGTGGGCGATGGCGCGGTGGGCAAGAGCAGCCTCATCGTCAGCTACACCTGCA
  ATGGATACCCCTCGCGCTATCGGCCTACAGCACTGGACACTTTCTCCGTGCAAGTCCTGGTAGATGGAGC
  CCCTGTGCGAATTGAGCTCTGGGACACAGCAGGGCAGGAGGACTTTGACCGGCTTCGTTCTCTCTGCTAC
  CCGGATACCGATGTCTTTCTGGCTTGCTTCAGCGTGGTGCAGCCCAGCTCCTTTCAAAACATAACAGAAA
  AATGGCTGCCGGAGATCCGCACTCACAACCCCCAAGCACCTGTGTTGCTGGTGGGCACTCAGGCCGACCT
  GAGGGACGATGTCAATGTACTAATTCAGTTGGACCAAGGAGGTCGGGAGGGCCCAGTACCCGAACCCCAA
  GCCCAGGGTTTGGCTGAGAAGATCCGGGCCTGCTGCTACCTTGAGTGCTCAGCCTTGACGCAGAAGAACT
  TGAAGGAGGTGTTCGACTCAGCCATTCTCAGTGCGATTGAGCACAAAGCCCGCCTGGAGAAGAAACTGAA
  CGCAAAAGGTGTGCGCACGCTCTCTCGCTGTCGCTGGAAGAAGTTCTTCTGCTTTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209798 representing NM_138542
  Red=Cloning site Green=Tags(s)
  
  MPPAGVPSLDRLSASGCGTCLPERRGAWSGLAPLLPRERAMPPRELSEAEPPPLPASTPPPRRRSAPPEL
  GIKCVLVGDGAVGKSSLIVSYTCNGYPSRYRPTALDTFSVQVLVDGAPVRIELWDTAGQEDFDRLRSLCY
  PDTDVFLACFSVVQPSSFQNITEKWLPEIRTHNPQAPVLLVGTQADLRDDVNVLIQLDQGGREGPVPEPQ
  AQGLAEKIRACCYLECSALTQKNLKEVFDSAILSAIEHKARLEKKLNAKGVRTLSRCRWKKFFCFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138542
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138542.2, NP_612551.2
• RefSeq Size: 1741 bp
• RefSeq ORF: 831 bp
• Locus ID: 171581
• UniProt ID: Q9Z1Y0
• Cytogenetics: 3q35
• MW: 30.3 kDa
• Gene Summary: Plays a role in the control of the actin cytoskeleton via activation of the JNK pathway.[UniProtKB/Swiss-Prot Function]