Cd38 (NM_013127) Rat Tagged ORF Clone

CAT#: RR209763

Cd38 (Myc-DDK-tagged ORF) - Rat CD38 molecule (Cd38), (10 ug)

Product Images

Circular map for RR209763

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd38
• Synonyms: ADPRC 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209763 representing NM_013127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACTATGAATTTTCCCAGGTGTCTGAGGACAGACCTGGCTGCCGCCTCACTAGGAAAGCCCAGA
  TCGGTCTTGGAGTGGGTCTCCTGCTCCTGGTCGCCTTGGTAGTGGTCGTGGTCATAGTTCTGTGGCCGCG
  CTCACCCCTGGTGTGGAAAGGGAAGCCTACCACGAAGCACTTCGCTGACATCATCTTGGGACGCTGCCTG
  ATCTATACTCAAATCCTCCGGCCGGAGATGAGAGATCAGGACTGCAAGAAGATACTAAGTACATTCAAAA
  GAGGGTTTATTTCCAAGAATCCTTGCAACATCACCAATGAAGACTACGCACCACTGGTTAAATTGGTTAC
  TCAAACCATACCATGTAACAAGACTCTCTTTTGGAGCAAGTCCAAACACCTGGCCCATCAGTATACTTGG
  ATCCAGGGGAAAATGTTCACCCTGGAGGACACACTGCTGGGCTATATTGCAGATGATCTCAGGTGGTGTG
  GAGACCCCAGTACTTCCGATATGAACTATGACTCTTGCCCACATTGGAGTGAAAATTGTCCCAACAACCC
  TGTTGCTGTGTTCTGGAATGTGATTTCCCAAAAGTTTGCAGAAGATGCCTGTGGTGTGGTCCAAGTGATG
  CTCAATGGGTCCCTCAGTGAGCCATTTTACAGAAACAGCACCTTTGGAAGTGTGGAAGTCTTTAATTTGG
  ACCCAAATAAGGTTCATAAACTACAGGCCTGGGTAATGCATGACATTAAAGGAACTTCCAGTAATGCATG
  TTCGAGCCCCTCCATAAATGAGCTGAAGTCGATTGTGAACAAAAGGAATATGATATTTGCCTGCCAGGAT
  AACTACCGACCTGTAAGATTTCTTCAGTGTGTGAAGAATCCTGAGCATCCATCATGTAGACTTAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209763 representing NM_013127
  Red=Cloning site Green=Tags(s)
  
  MANYEFSQVSEDRPGCRLTRKAQIGLGVGLLLLVALVVVVVIVLWPRSPLVWKGKPTTKHFADIILGRCL
  IYTQILRPEMRDQDCKKILSTFKRGFISKNPCNITNEDYAPLVKLVTQTIPCNKTLFWSKSKHLAHQYTW
  IQGKMFTLEDTLLGYIADDLRWCGDPSTSDMNYDSCPHWSENCPNNPVAVFWNVISQKFAEDACGVVQVM
  LNGSLSEPFYRNSTFGSVEVFNLDPNKVHKLQAWVMHDIKGTSSNACSSPSINELKSIVNKRNMIFACQD
  NYRPVRFLQCVKNPEHPSCRLNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013127
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013127.1, NP_037259.1
• RefSeq Size: 2248 bp
• RefSeq ORF: 912 bp
• Locus ID: 25668
• UniProt ID: Q64244
• Cytogenetics: 14q21
• MW: 34.4 kDa
• Gene Summary: ADP-ribosyl cyclase enzyme involved in regulation of cellular calcium levels [RGD, Feb 2006]