Nt5e (NM_021576) Rat Tagged ORF Clone

CAT#: RR209759

Nt5e (Myc-DDK-tagged ORF) - Rat 5' nucleotidase, ecto (Nt5e), (10 ug)

Product Images

Circular map for RR209759

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nt5e
• Synonyms: CD73; Nt5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209759 representing NM_021576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTCCCGCGGCTGCTACGGCACCCAAGTGGCTGCTTCTCGCGCTAAGCGCTCTACTACCACTGTGGC
  CCACAGCCAAATCCTGGGAGCTCACGATCCTGCACACAAACGACGTGCACAGCCGGCTAGAGCAAACCAG
  CGATGACTCCACCAAGTGCCTCAACGCAAGCCTGTGTGTGGGCGGCGTGGCCCGGCTCTTCACCAAGGTG
  CAGCAGATCCGCAAGGAAGAACCCAACGTACTGCTTTTGGATGCTGGCGATCAGTACCAGGGCACCATCT
  GGTTCACTGTTTACAAAGGCCTGGAAGTGGCACACTTCATGAACCTCCTGGGCTACGATGCCATGGCACT
  GGGAAATCATGAATTTGATAACGGTGTGGAAGGACTGATTGATCCCCTCCTCAGAAATGTCAAATTTCCA
  ATTCTGAGTGCAAATATTAAGGCCCGGGGGCCACTAGCACCTCAGATATCCGGACTTTATTTGCCATATA
  AAGTTCTCTCTGTCGGTGGTGAGGTTGTGGGGATTGTTGGATATACTTCAAAGGAAACCCCCTTCCTCTC
  AAATCCAGGGACAAATTTAGTCTTCGAAGATGAAGTCACTGCGTTGCAGCCTGAAGTGGATAAACTAAAA
  ACTCTAAACGTGAACAAGATCATTGCCCTGGGACACTCTGGTTTCGAGATGGATAAACTCATTGCCCAGA
  AGGTGAGAGGCGTGGACGTCGTGGTGGGAGGACACACCAACACCTTTCTCTACACAGGAAATCCACCTTC
  CAAAGAAGTGCCTGCTGGGAAGTACCCATTCATAGTCACCTCTGACGATGGGCGAAAGGTTCCCGTGGTC
  CAGGCCTATGCCTTTGGCAAATACCTGGGCTATCTGAAGGTTGAGTTTGATGATAAAGGAAATGTTGTCA
  CTTCCTATGGAAATCCCATTCTTCTGAACAGCACCATTCGTGAAGATGCAGCCATCAAAGCAGACATTAA
  CCAGTGGAGGATAAAATTAGATAATTATTCTACACAGGAACTCGGGAGAACCATCGTCTACCTGAATGGC
  TCCGCTCAGGAGTGCCGCTTCAGAGAGTGCAACATGGGAAACCTGATCTGTGATGCCATGATTAACAACA
  ACCTCAGACACCCAGATGAAATGTTTTGGAACCACGTGTCCATGTGCATTGTAAATGGAGGTGGCATCCG
  GTCCCCCATTGATGAGAGGAACAATGGTACCATCACCTGGGAGAACCTGGCTGCTGTGCTGCCCTTTGGA
  GGGACATTTGACCTTGTCCAATTGAAAGGGTCCACCCTGAAGAAGGCTTTTGAGCACAGTGTGCATCGAT
  ATGGCCAGTCCACAGGGGAGTTCCTGCAAGTGGGTGGAATCCATGTGGTGTATGATATTTCCCGAAAGCC
  CTGGGACAGAGTGGTCCAATTAAAAGTTCTCTGCACCAAGTGTCGAGTGCCCATCTATGAGCCTCTCGAA
  ATGGATAAAGTGTATAAAGTGGTCCTCCCAAGCTATCTGGTCAACGGTGGGGATGGATTCCAAATGATAA
  AAGATGAATTATTAAAACATGACTCTGGTGACCAAGATATCAGTGTGGTTTCTGAATACATCTCAAAAAT
  GAAAGTAATTTACCCAGCAGTTGAAGGACGGATCAAGTTCTCTGCAGCAAGTCATTACCAAGGAAGCTTT
  CCTTTAATAATTCTTTCCTTTTGGGCAGTGATCCTTGTTTTGTACCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209759 representing NM_021576
  Red=Cloning site Green=Tags(s)
  
  MRPAAATAPKWLLLALSALLPLWPTAKSWELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKV
  QQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFP
  ILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLK
  TLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVV
  QAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNG
  SAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFG
  GTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLE
  MDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIKFSAASHYQGSF
  PLIILSFWAVILVLYQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021576
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021576.2, NP_067587.2
• RefSeq Size: 2826 bp
• RefSeq ORF: 1731 bp
• Locus ID: 58813
• Cytogenetics: 8q31
• MW: 64 kDa
• Gene Summary: may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]