Rack1 (NM_130734) Rat Tagged ORF Clone

CAT#: RR209746

Gnb2l1 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 (Gnb2l1), (10 ug)

Product Images

Circular map for RR209746

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rack1
• Synonyms: Gnb2l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209746 representing NM_130734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGCAAATGACCCTTCGTGGGACCCTCAAGGGCCATAATGGATGGGTTACACAGATCGCCACCA
  CTCCGCAGTTCCCGGACATGATCCTGTCGGCGTCTCGAGACAAGACCATCATCATGTGGAAGCTGACCAG
  GGATGAGACCAACTACGGCATACCACAACGTGCTCTTCGAGGTCACTCCCACTTTGTTAGCGATGTTGTC
  ATCTCCTCTGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTACGCCTCTGGGATCTCACAA
  CGGGCACTACCACGAGACGATTTGTCGGCCACACCAAGGATGTGCTGAGCGTGGCTTTCTCCTCTGACAA
  CCGGCAGATTGTCTCTGGGTCCCGAGACAAGACCATTAAGTTATGGAATACTCTGGGTGTCTGCAAGTAC
  ACTGTCCAGGATGAGAGTCATTCAGAATGGGTGTCTTGTGTCCGCTTCTCCCCGAACAGCAGCAACCCTA
  TCATCGTCTCCTGCGGATGGGACAAGCTGGTCAAGGTGTGGAATCTGGCTAACTGCAAGCTAAAGACCAA
  CCACATTGGCCACACTGGCTATCTGAACACAGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGA
  GGCAAGGATGGCCAGGCTATGCTGTGGGATCTCAATGAAGGCAAGCACCTTTACACATTAGATGGTGGAG
  ACATCATCAATGCCTTGTGCTTCAGCCCCAACCGCTACTGGCTCTGTGCTGCCACTGGCCCCAGTATCAA
  GATCTGGGACTTGGAGGGCAAGATCATTGTAGATGAACTGAAGCAAGAGGTTATCAGCACCAGCAGCAAG
  GCAGAGCCACCCCAGTGTACCTCTTTGGCTTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTATACCG
  ACAACTTGGTGCGTGTATGGCAGGTGACTATTGGTACCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209746 representing NM_130734
  Red=Cloning site Green=Tags(s)
  
  MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVV
  ISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKY
  TVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASG
  GKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSK
  AEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130734
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130734.2, NP_570090.2
• RefSeq Size: 1209 bp
• RefSeq ORF: 954 bp
• Locus ID: 83427
• UniProt ID: P63245
• Cytogenetics: 10q21
• MW: 35.1 kDa
• Gene Summary: may act as a receptor for activated protein kinase C [RGD, Feb 2006]