Lpin1 (NM_001012111) Rat Tagged ORF Clone

CAT#: RR209657

Lpin1 (Myc-DDK-tagged ORF) - Rat lipin 1 (Lpin1), (10 ug)

Product Images

Circular map for RR209657

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lpin1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209657 representing NM_001012111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACGTGGGGCAGTTGGCCGGCCAGGTGTTTGTGACGGTGAAGGAGCTCTACAAGGGACTGAACC
  CTGCCACGCTGTCAGGATGCATCGACATCATTGTTGTCCGCCAGCCCAATGGCAGTCTGCAGTGCTCCCC
  GTTCCACGTCCGCTTCGGGAAGATGGGTGTCCTACGCTCCCGAGAGAAAGTGGTGGACATAGAAATCAAT
  GGGGAGTCTGTGGATTTGCACATGAAGTTGGGAGACAACGGAGAAGCGTTTTTTGTTCAAGAGACTGACA
  ACGATCAGGAAGTCATCCCCATGTACCTAGCCACGTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGA
  AAGCCAGCTGAAAAGGAACTCTGTGGACAGAATCCGGGGCCTGGATCCCACTACAGCTGCCCAGGGCCTG
  CCTCCCAGCGACACCCCATCCACTGGTTCTCTGGGGAAGAAGAGAAGGAAAAGGAGGAGGAAGGCCCATT
  TGGACAATCTCAAAAGAGATGACAATGTCAACACATCTGAGGACGAGGACATGTTTCCCATAGAGATGAG
  CTCGGATGAGGACACAGCACTGATGGATGGGAGCAGAGCTCTTCCTAATGATGTACCACCATTCCAAGAT
  GATATTCCTAAGGAAAACCTCCCCTCGATTTCAACATATCCCCAGTCAGCATCGTACCCCAATTCAGACA
  GAGAGTGGTCCCCCAGCCCCAGCAGCCTGGTAGATTGTCAGAGGACTCCCCCTCACCTGGCCGAGGGAGT
  TCTCTCTAGCTCTTGTCCTCTGCAGTCTTGCCACTTCCATGCTTCGGAAAGTCCTTCAGGCTCCCGGCCC
  TCAACACCAAAAAGTGATTCTGAGCTGGTCAGTAAGTCTGCAGACAGATTGACGCCAAAGAATAACCTGG
  AAATGCTCTGGCTATGGGGTGAATTGCCACAGGCTGCAAAGTCGTCTTCTCCACACAAGATGAAAGACTC
  CAGCCCCTTAGGAGGTCGGAAGATGGCTGATAAAATCCACTTCCAGACCATTCACAGCGAGTCTTCAGAC
  ACTTTCAGTGACCAGTCCCCAACAATGGCCCGGGGGCTGCTCATCCACCAGAGCAAGGCTCAGACGGAAA
  TGCAGTTTGTGAATGAGGAGGACCTTGAGGCCTTGGGGGCCGCAGCCCCACCTTTGTCTGTAGCCGAAGA
  GCTCAAGGCCCCAGCCTCCAGCACAGCACAGACATCAAGCAAGTCAGATTCCCCTTCCAGAAAAAAAGAT
  AAACGAAGCCGGCACCTCGGAGCTGATGGCGTTTATCTGGATGACCTCACAGACATGGACCCGGAAGTGG
  CTGCCCTGTATTTCCCCAAGAATGGGGATCCCGGTGGGCTTCCCAAACAAGCGAGTGACAACGGAGCCAG
  GTCAGCCAACCAGTCCCCACAGTCGGTGGGTAGCTCGGGCATTGACAGCGGCGTGGAGAGCACCTCCGAC
  AGCCTGAGGGACCTGCCGTCCATCGCCATCTCCCTCTGCGGTGGCCTCAGTGACCACAGAGAGATCACCA
  AAGATGCATTTTTGGAACAAGCCGTGTCATATCAGCAATTTGCCGACAACCCTGCTATCATCGATGATCC
  CAACCTCGTGGTCAAGATTGGTAATAAGTACTACAACTGGACAACAGCAGCTCCTCTACTTCTGGCGATG
  CAGGCTTTCCAGAAACCTTTGCCAAAGGCCACTGTGGAATCCATCATGAGAGATAAGATGCCCAAAAAGG
  GAGGGAGATGGTGGTTTTCCTGGAGAGGAAGAAACGCCACAATCAAAGAGGAGAGCAAGCCTGAGCAGGG
  CCTGCCTGGGAAGGGCCACAATACCGGAGAGCAGCCTGCACAGCTTGGCCTGACCACCAGAATAAAGCAT
  GAGTCATCCTCTAGTGATGAAGAGCACTCAGCTACCAAGCCATCGAGCTCGAGCCACCTCTCTCTCTTGT
  CCAACGTCAGCTACAAAAAGACCCTGCGGCTCACATCGGAGCAGTTGAAAAGCTTGAAGTTGAAGAATGG
  CCCCAACGATGTGGTGTTCAGTGTCACTACCCAGTACCAGGGCACCTGTCGCTGTGAGGGTACCATCTAC
  CTGTGGAATTGGGATGACAAAGTCATCATCTCAGATATCGATGGTACCATCACAAGATCTGATACTCTTG
  GTCACATATTGCCCACACTGGGAAAGGATTGGACTCACCAGGGCATCGCAAAGCTGTATCACAAAGTAAG
  CCAGAATGGCTACAAGTTTCTCTATTGTTCTGCACGTGCCATTGGGATGGCAGACATGACGAGGGGCTAT
  CTGCACTGGGTCAACGAGAGGGGCACAGTGCTGCCACAGGGGCCGCTTCTGCTGAGCCCCAGCAGCCTCT
  TCTCCGCCTTGCACAGAGAAGTGATTGAAAAGAAGCCAGAAAAGTTCAAAGTCCAGTGTTTGACAGACAT
  CAAAAACCTGTTTTTCCCAAACACAGAGCCCTTTTATGCTGCTTTTGGGAACCGGCCTGCCGATGTGTAT
  TCCTACAAGCAAGTGGGAGTGTCCCTGAATAGAATCTTCACCGTCAACCCCAAGGGTGAGCTGGTGCAGG
  AGCATGCCAAGACCAACATCAGCTCGTATGTGCGGCTCTGCGAAGTGGTCGACCACGTCTTCCCCTTGCT
  GAAGAGAAGCCATTCCTGCGACTTCCCCTGTTCAGACACTTTCAGTAACTTCACCTTTTGGAGAGAGCCA
  CTGCCACCCTTTGAAAACCAGGACGTGCATTCAGCCTCAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209657 representing NM_001012111
  Red=Cloning site Green=Tags(s)
  
  MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVVRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEIN
  GESVDLHMKLGDNGEAFFVQETDNDQEVIPMYLATSPILSEGASRMESQLKRNSVDRIRGLDPTTAAQGL
  PPSDTPSTGSLGKKRRKRRRKAHLDNLKRDDNVNTSEDEDMFPIEMSSDEDTALMDGSRALPNDVPPFQD
  DIPKENLPSISTYPQSASYPNSDREWSPSPSSLVDCQRTPPHLAEGVLSSSCPLQSCHFHASESPSGSRP
  STPKSDSELVSKSADRLTPKNNLEMLWLWGELPQAAKSSSPHKMKDSSPLGGRKMADKIHFQTIHSESSD
  TFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLEALGAAAPPLSVAEELKAPASSTAQTSSKSDSPSRKKD
  KRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPGGLPKQASDNGARSANQSPQSVGSSGIDSGVESTSD
  SLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFADNPAIIDDPNLVVKIGNKYYNWTTAAPLLLAM
  QAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNATIKEESKPEQGLPGKGHNTGEQPAQLGLTTRIKH
  ESSSSDEEHSATKPSSSSHLSLLSNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIY
  LWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGY
  LHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVY
  SYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREP
  LPPFENQDVHSASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012111
• ORF Size: 2772 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012111.1, NP_001012111.1
• RefSeq Size: 3107 bp
• RefSeq ORF: 2775 bp
• Locus ID: 313977
• Cytogenetics: 6q16
• MW: 101.9 kDa