Srsf2 (NM_001009720) Rat Tagged ORF Clone

CAT#: RR209643

Srsf2 (Myc-DDK-tagged ORF) - Rat splicing factor, arginine/serine-rich 2 (Sfrs2), (10 ug)

Product Images

Circular map for RR209643

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srsf2
• Synonyms: Sfrs2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209643 representing NM_001009720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACGGCCGCCCGCCTCCCGATGTGGAGGGCATGACCTCCCTCAAGGTGGACAACCTGACCTACC
  GCACCTCACCCGACACCCTGAGGCGCGTCTTCGAGAAGTACGGGCGCGTCGGCGACGTGTACATCCCGCG
  GGACCGCTACACCAAGGAGTCCCGTGGCTTCGCTTTCGTCCGGTTCCACGACAAGCGTGACGCCGAAGAC
  GCCATGGACGCCATGGATGGGGCCGTGCTCGATGGCCGCGAGCTGCGGGTGCAGATGGCGCGCTACGGCC
  GTCCTCCCGACTCGCACCACAGCCGCCGGGGACCGCCACCCCGTAGGTACGGCGGCGGCGGCTACGGGCG
  ACGGAGCCGCAGTCCTCGGCGCCGCCGGCGCAGCCGATCCCGGAGCCGGAGCCGTTCCAGGTCCCGAAGC
  CGCTCTCGCTACAGCCGCTCCAAGTCTCGGTCCCGCACTCGTTCTCGCTCGAGGTCGACCTCCAAGTCCA
  GATCTGCCCGAAGATCCAAGTCCAAGTCCTCGTCGGTCTCCAGATCCCGCTCGAGGTCCAGGTCCAGGTC
  GCGATCCAGAAGTCCTCCGCCTGTGTCGAAGAGAGAGTCCAAGTCTAGGTCGCGGTCCAAGAGCCCACCC
  AAGTCTCCAGAAGAAGAGGGAGCGGTTTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209643 representing NM_001009720
  Red=Cloning site Green=Tags(s)
  
  MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAED
  AMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRS
  RSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPP
  KSPEEEGAVSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009720
• ORF Size: 663 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009720.2, NP_001009720.1
• RefSeq Size: 1901 bp
• RefSeq ORF: 666 bp
• Locus ID: 494445
• UniProt ID: Q6PDU1
• Cytogenetics: 10q32.2
• MW: 25.5 kDa
• Gene Summary: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment (By similarity).[UniProtKB/Swiss-Prot Function]