Fjx1 (NM_001108955) Rat Tagged ORF Clone

CAT#: RR209480

Fjx1 (Myc-DDK-tagged ORF) - Rat four jointed box 1 (Drosophila) (Fjx1), (10 ug)

Product Images

Circular map for RR209480

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fjx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209480 representing NM_001108955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGAAGATGCGGGGCGCCGCCGCCGCGGGGCTCTGGCTGCTGGCTTTGGGCTCGCTGCTAACGC
  TGTGGGGAGGGCTCCTGCCACCACGGACCGAGCTGCCAGCCTCCCGGCCGCCGGAAGATCGACTCCCTCG
  GCATCCGATCCAGAGTGGCGGCCCCGCGCCCGAGCCGCGATTCCCTCTGCCCCCGCCCCTAGTGTGGGAC
  GCCCGCGGCGGCTCCCTGAAAACTTTCCGGGCGCTGCTCACCCTGGCGGCCGGCGCGGATAACCCGCCTG
  GGAGGCACCCGGACGATCGCGGGCGGCACGTGCCCTCCGGGCTGTCCTGGCCAGCGGAGCACACGGCAGT
  GCACGGGGGCGTGTTTTGGAGCCGCGGCCTGGAGGAGCAGGTGCCCCGGGGCTTTTCCGAAGCCCAAGCA
  GCAGCTTGGCTGGAGGTGGCACGGGGAGCTCGGGTGGTGGCTCTGGATCGCGGGGGCTGTGGACGCAGTT
  CCAACCGCCTAGCCCGCTTTGCCGACGGCACCCGTGCCTGTGTACGCTACGGCATCAACCCAGAGCAGAT
  ACAGGGCGAGGCCCTGTCCTACTACCTTGCGCGCCTGCTGGGCCTCCAGCGCCACGTGCCGCCGCTGGCA
  CTGGCTCGGGTGGAGGCTCGGGGCGCGCAGTGGGTGCAGGTGCAGGAGGAGCTGCGCACGGCGCACTGGA
  CCGAGGGCAGCGTGGTGAGCCTGACGCGCTGGCTGCCTAACCTCACCGACGTGGTGGTGCCCGAGCCCTG
  GCGATCAGAGGACGGCCGTCTGCGGCCCCTGCGCGACGCCGGGGGCGAGCTGACCAACCTCAGCCAGGCG
  GAGCTGGTGGACCTGGTACAATGGACCGATCTGATCCTCTTCGATTACCTGACAGCCAACTTCGACCGGC
  TTGTAAGCAACCTCTTCAGCTTACAGTGGGACCCACGCGTTATGCACCGCGCTACAAGCAACCTGCACCG
  AGGACCAGGAGGGGCGTTGGTCTTTCTGGACAATGAGGCGGGCCTGGTGCACGGGTACCGGGTAGCCGGC
  ATGTGGGACAAGTATAACGAACCGCTGCTACAGTCGGTGTGTGTATTCCGAGAGCGGACTGCTAGGCGCG
  TCTTGGAGCTGCACCGGGGTCAGGACGCGGCAGCCCGGCTCCTGCGCCTCTACAGTCGCCACGAACCGCG
  TTTCCCAGAGCTGGCGGAGCTCGCAGACCCCCACGCTCAGCTGCTACAGCGCCGCCTTGACTTCCTCGCC
  AAACACATTTTGCACTGTAAGGCCAAGTACGGCCGCCGACCCGGGGACTTAATAACACTCCGAGGAAGAG
  AGGGACTGGGTTATGAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209480 representing NM_001108955
  Red=Cloning site Green=Tags(s)
  
  MGRKMRGAAAAGLWLLALGSLLTLWGGLLPPRTELPASRPPEDRLPRHPIQSGGPAPEPRFPLPPPLVWD
  ARGGSLKTFRALLTLAAGADNPPGRHPDDRGRHVPSGLSWPAEHTAVHGGVFWSRGLEEQVPRGFSEAQA
  AAWLEVARGARVVALDRGGCGRSSNRLARFADGTRACVRYGINPEQIQGEALSYYLARLLGLQRHVPPLA
  LARVEARGAQWVQVQEELRTAHWTEGSVVSLTRWLPNLTDVVVPEPWRSEDGRLRPLRDAGGELTNLSQA
  ELVDLVQWTDLILFDYLTANFDRLVSNLFSLQWDPRVMHRATSNLHRGPGGALVFLDNEAGLVHGYRVAG
  MWDKYNEPLLQSVCVFRERTARRVLELHRGQDAAARLLRLYSRHEPRFPELAELADPHAQLLQRRLDFLA
  KHILHCKAKYGRRPGDLITLRGREGLGYE
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108955
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108955.1, NP_001102425.1
• RefSeq Size: 2102 bp
• RefSeq ORF: 1350 bp
• Locus ID: 366140
• Cytogenetics: 3q32
• MW: 50 kDa