Wnt5a (NM_022631) Rat Tagged ORF Clone

CAT#: RR209326

Wnt5a (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), (10 ug)

Product Images

Circular map for RR209326

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt5a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209326 representing NM_022631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGCCCATTGGAATATTAAGCCCAGGAGTGGCTTTGGGGACCGCTGGAGGTGCCATGTCTTCCA
  AGTTCTTCCTAATGGCTTTGGCCACATTTTTCTCCTTCGCCCAGGTTGTAATAGAAGCTAATTCTTGGTG
  GTCCCTAGGTATGAATAACCCTGTTCAGATGTCAGAAGTATACATCATAGGAGCACAGCCTCTCTGCAGC
  CAACTGGCGGGACTTTCTCAAGGACAGAAGAAACTCTGCCACTTGTATCAGGACCACATGCAGTACATTG
  GAGAAGGCGCGAAGACGGGCATCAAAGAGTGCCAGTACCAGTTCCGGCATCGGAGATGGAACTGCAGCAC
  AGTGGACAACACTTCTGTCTTTGGCAGGGTGATGCAAATAGGCAGCCGAGAGACAGCCTTCACGTACGCG
  GTGAGCGCTGCTGGAGTGGTAAATGCCATGAGCCGAGCATGTCGGGAAGGCGAGCTGTCTACCTGTGGTT
  GCAGCCGCGCAGCACGCCCCAAGGACTTACCTCGGGACTGGCTGTGGGGCGGTTGCGGGGACAACATCGA
  CTATGGCTACCGCTTCGCCAAGGAATTCGTGGACGCACGAGAAAGGGAACGAATCCACGCCAAGGGCTCC
  TATGAGAGCGCACGCATCCTCATGAACTTGCACAACAATGAAGCAGGTCGCAGGACAGTATACAACCTGG
  CAGATGTAGCCTGTAAGTGCCATGGAGTGTCTGGCTCCTGTAGCCTCAAGACATGCTGGCTGCAGCTGGC
  GGACTTCCGCAAGGTGGGCGATGCCCTCAAGGAGAAGTATGACAGCGCAGCGGCCATGAGGTTGAACAGC
  CGGGGCAAGCTGGTACAGGTCAACAGCCGCTTCAACTCCCCAACCACGCAGGACCTGGTCTACATCGACC
  CAAGTCCGGACTACTGTGTGCGCAACGAGAGCACTGGCTCACTGGGCACGCAGGGACGCCTGTGCAACAA
  GACCTCAGAGGGGATGGACGGCTGCGAGCTCATGTGCTGTGGGCGTGGCTATGACCAGTTTAAGACGGTG
  CAGACCGAACGCTGCCATTGCAAGTTTCACTGGTGCTGCTATGTCAAGTGTAAAAAGTGCACGGAGATTG
  TGGATCAGTTCGTGTGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209326 representing NM_022631
  Red=Cloning site Green=Tags(s)
  
  MKKPIGILSPGVALGTAGGAMSSKFFLMALATFFSFAQVVIEANSWWSLGMNNPVQMSEVYIIGAQPLCS
  QLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYA
  VSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGS
  YESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNS
  RGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTV
  QTERCHCKFHWCCYVKCKKCTEIVDQFVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022631
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022631.2, NP_072153.2
• RefSeq Size: 5422 bp
• RefSeq ORF: 1143 bp
• Locus ID: 64566
• Cytogenetics: 16p16
• MW: 42.3 kDa
• Gene Summary: mouse homolog regulates chondrocyte differentiation and proliferation in longitudinal skeletal growth of long bones [RGD, Feb 2006]