RGD1306441 (NM_001106053) Rat Tagged ORF Clone

CAT#: RR209157

RGD1306441 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4921530L21 (RGD1306441), (10 ug)

Product Images

Circular map for RR209157

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGD1306441
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209157 representing NM_001106053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATCTCTGGATCAAAACAAATACACAAAAGGAAAATACTGCGGTTGTTTTCTTGTTTCTCTTGTA
  CCCAAAATCAAAAGAAACTCAAACATAAATCCCAGGACGTGGAAAAAAAACTCTGTAGAGAAAATAAGGT
  GTTGAGAGATGAAAATAGGGCTCTGAGAAAAGACAACAAATTTCTCTGGGGCGAAAACAAAGCCTTAGGA
  AGAGAAAATAAAACCTTCCGGATGGACAACCAGTTCATCAGGGAAAGAAATCATATCCTAAGGCAGCAGA
  ACCAGCTACTCCGGAAGGTGAAAAGGTTGATTTCAGAGAACCCAAAACTGTCTGGGGAAGAATGCAATGC
  CTTGAACACAGAAGGAAGGTCTTTTTGGCAACAGAATCGAGCCATGGAAGCTCAGATCACAGCTCTCCGG
  CAGCAAGAGAAAGCCTTCCAGAATGAGGCCAAAGCCTTGCATGAGGAGATCAAGTCCCTGTGTGAGGAGA
  CCAAGGCTCTACAACACCAAGAGAGGGCTCTCAGGATGGAAGAGAAAGCCCTGCTGAGGGACGGAGTGGC
  TGCAGAGCTGGCAGAGGCCCTGACCAAGGAGGGAGCTGCTCTAGAGATGGAAGAACAGGCTTTGTGGAAG
  GAAGAGCAGGCTCTCAGGGAGGAGAACAAGGCGCTGAGAGAAGAGCACGGGGCTCTACAAGACGAGGAAG
  TTGCCCTGCAGGAAGAGGCGAGAATCCTCCAGGAGTGGAATAATCTTCTTCAGGGGAAGATCACAAACAA
  CCTCCCAGAGAATATGAATAACCAAGACCCAGAGAAGTGCGAACTAAGAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209157 representing NM_001106053
  Red=Cloning site Green=Tags(s)
  
  MNISGSKQIHKRKILRLFSCFSCTQNQKKLKHKSQDVEKKLCRENKVLRDENRALRKDNKFLWGENKALG
  RENKTFRMDNQFIRERNHILRQQNQLLRKVKRLISENPKLSGEECNALNTEGRSFWQQNRAMEAQITALR
  QQEKAFQNEAKALHEEIKSLCEETKALQHQERALRMEEKALLRDGVAAELAEALTKEGAALEMEEQALWK
  EEQALREENKALREEHGALQDEEVALQEEARILQEWNNLLQGKITNNLPENMNNQDPEKCELRM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106053
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106053.1, NP_001099523.1
• RefSeq Size: 1134 bp
• RefSeq ORF: 825 bp
• Locus ID: 290425
• Cytogenetics: 15q21
• MW: 32.2 kDa