Ap2b1 (NM_080583) Rat Tagged ORF Clone

CAT#: RR209156

Ap2b1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap2b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209156 representing NM_080583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCCAAGTACTTCACAACCAATAAGAAGGGAGAAATCTTTGAATTAAAAGCTGAACTCAACA
  ATGAAAAGAAAGAAAAGAGGAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATGT
  GAGCTCTCTCTTCCCAGATGTGGTGAACTGTATGCAGACTGACAACCTGGAACTAAAGAAGCTTGTGTAC
  CTCTATCTGATGAACTATGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTCAACAGCTTTGTGAAGG
  ATTGTGAAGACCCCAATCCTTTGATTCGAGCCTTGGCAGTTAGAACCATGGGATGCATCCGGGTGGACAA
  GATTACAGAGTATCTCTGTGAACCCCTCCGCAAGTGCTTGAAGGATGAAGACCCCTATGTTCGGAAAACA
  GCAGCAGTATGCGTGGCAAAACTCCATGATATCAATGCCCAGATGGTGGAAGATCAGGGATTTCTGGATT
  CTCTGCGGGATCTCATAGCAGATTCAAACCCAATGGTGGTGGCTAATGCTGTAGCAGCATTGTCTGAGAT
  CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCTCAGAATATCAATAAGCTGCTCACAGCC
  CTGAATGAGTGCACTGAGTGGGGCCAGATTTTCATCTTGGACTGCCTGTCTAATTACAACCCTAAAGATG
  ACCGGGAAGCTCAGAGCATCTGTGAGCGAGTGACGCCTCGGCTCTCTCATGCCAATTCTGCAGTGGTGCT
  TTCAGCAGTAAAAGTTCTGATGAAGTTTCTAGAGTTGTTACCCAAGGACTCTGACTACTACAATATGCTG
  CTAAAGAAGCTAGCGCCTCCACTTGTCACTTTGCTCTCTGGGGAGCCAGAAGTGCAGTATGTTGCCCTGA
  GGAACATCAACCTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAGGTCTTCTTTGTGAA
  GTACAATGATCCTATCTATGTTAAACTAGAGAAGTTAGACATCATGATTCGTCTTGCATCCCAAGCCAAC
  ATTGCTCAGGTTCTGGCAGAACTGAAGGAATATGCCACTGAAGTTGATGTGGACTTTGTTCGCAAAGCTG
  TGAGGGCCATTGGACGGTGTGCCATCAAAGTGGAGCAATCAGCAGAACGCTGTGTGAGCACACTGCTTGA
  TCTAATCCAGACCAAAGTAAATTATGTGGTCCAAGAGGCAATTGTTGTCATCAGGGACATCTTCCGAAAA
  TACCCCAACAAGTATGAGAGCATTATCGCCACGCTGTGTGAGAACTTGGACTCCCTGGATGAACCCGATG
  CCCGAGCGGCTATGATTTGGATTGTAGGAGAGTATGCTGAAAGAATCGATAATGCCGATGAGTTACTAGA
  GAGCTTCCTGGAAGGTTTTCATGATGAAAGCACCCAGGTGCAGCTCACGTTGCTTACCGCCATAGTGAAA
  CTGTTTCTCAAGAAGCCATCAGAAACACAGGAGCTGGTCCAACAGGTCTTGAGCTTGGCCACACAGGATT
  CTGATAATCCTGACCTTCGAGATCGGGGTTATATTTATTGGCGCCTTCTTTCAACTGACCCTGTGACAGC
  CAAAGAAGTAGTGTTGTCTGAGAAGCCATTGATCTCTGAGGAAACAGACCTCATTGAACCTACCCTCCTG
  GATGAGCTCATCTGCCACATTGGTTCTTTGGCCTCCGTGTACCATAAACCTCCGAATGCTTTTGTGGAAG
  GGAGCCATGGCATTCATCGCAAACACTTGCCAATTCACCATGGGAGCACTGATGCAGGTGATAGCCCTGT
  TGGCACCACCACTGCAACCAACCTGGAACAGCCTCAGGTCATCCCCTCTCAAGGTGACCTTCTGGGGGAT
  CTTTTAAATCTTGACCTGGGTCCCCCAGTGAATGTCCCGCAAGTGTCCTCCATGCAGATGGGAGCAGTGG
  ATCTTTTAGGAGGAGGACTGGATAGCCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGC
  AGTAGGACAGTCCTTCATCCCGTCATCAGTGCCTGCAACCTTCGCTCCTTCACCTACTCCTGCTGTGGTC
  AGCAGTGGTCTGAATGACCTGTTTGAGCTTTCCACTGGGATAGGCATGGCACCTGGCGGATATGTGGCTC
  CTAAGGCAGTCTGGCTACCTGCTGTAAAGGCTAAAGGCTTGGAGATTTCGGGGACGTTTACTCACCGCCA
  AGGGCACATCTATATGGAAATGAACTTCACCAACAAAGCTCTGCAGCACATGACGGATTTTGCCATCCAG
  TTTAACAAGAATAGCTTCGGTGTCATCCCGAGCACTCCCTTGGCCATACATACTCCGCTGATGCCAAACC
  AGAGCATTGATGTGTCTCTGCCTCTCAACACCTTGGGCCCAGTCATGAAGATGGAGCCTCTGAATAACTT
  GCAGGTGGCTGTTAAAAACAATATTGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTT
  GTAGAAGATGGCAAAATGGAACGCCAGGTCTTCCTTGCGACGTGGAAGGATATTCCCAATGAAAATGAGC
  TCCAATTTCAGATTAAGGAGTGTCATTTAAACGCTGACACAGTTTCCAGCAAGTTGCAAAACAACAATGT
  TTACACTATCGCCAAGAGGAATGTGGAGGGGCAGGACATGCTGTACCAGTCCCTGAAGCTCACTAATGGC
  ATTTGGATTTTGGCAGAGCTGCGGATCCAGCCAGGAAACCCCAATTATACGCTGTCGCTGAAGTGTAGAG
  CCCCTGAAGTCTCTCAGTACATCTATCAGGTCTACGACAGCATTTTGAAAAACCCCCTGAAGTCTCTCAG
  TACATCTATCAGGTCTACGACAGCATTTTGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209156 representing NM_080583
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA
  LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML
  LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK
  YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK
  LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL
  DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGD
  LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVV
  SSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQ
  FNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLF
  VEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNG
  IWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKNPLKSLSTSIRSTTAF*K
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080583
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5413 bp
• RefSeq ORF: 2856 bp
• Locus ID: 140670
• UniProt ID: P62944
• Cytogenetics: 10q26
• MW: 105.7 kDa
• Gene Summary: subunit of the clathrin-associated protein complex 2 [RGD, Feb 2006]