Pnmt (NM_031526) Rat Tagged ORF Clone

CAT#: RR209077

Pnmt (Myc-DDK-tagged ORF) - Rat phenylethanolamine-N-methyltransferase (Pnmt), (10 ug)

Product Images

Circular map for RR209077

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pnmt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209077 representing NM_031526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGTGGCTCAGACCCGAAGCACACTGCAGGGATGGACTCTGACTCCGACCCTGGCCAGGCAGAGG
  TCGCCTTGGCTTACCAGCGCTTTGAGCCCCGTGCCTACCTCCGCAACAACTACGCGCCTCCTCGTGGGGA
  CCTGAGCAACCCTGATGGTGTGGGGCCTTGGAAGCTTCGCTGCATGGCACAAGTCTTTGCCACCGGTGAG
  GTGTCTGGACAGGTCCTCATTGACATCGGCTCAGGCCCCACCATATACCAGCTGCTCAGCGCCTGTGCCC
  ACTTCGAGGACATCACCATGACAGACTTCTTGGAGGTCAACCGGCAGGAGCTGGGACTCTGGCTGCGAGA
  GGAACCAGGAGCCTTCGACTGGAGTGTGTATAGCCAGCATGTCTGCCTCATCGAGGACAAGGGAGAGTCC
  TGGCAGGAGAAAGAACGCCAGCTCCGAGCGAGGGTGAAGCGAGTCTTGCCCATTGATGTGCACAAGCCCC
  AGCCCCTGGGAGCTTCGGGCCTGGCACCCCTGCCTGCTGACGCCTTGGTCTCTGCCTTCTGCCTGGAGGC
  TGTGAGCCCGGATCTCCCAAGCTTCCGGCAGGCTTTGTATCATATCACGACGCTGCTGAGGCCCGGGGGT
  AATCTCCTCTTCATCGGGGCCCTGGAGGAGTCGTGGTACCTTGCTGGGGAGGCCAGGCTATCTGTGGTTC
  CAGTGTCAGAGGAGGAGGTGAGGGAGGCCCTGGTCTGTAGTGGTTATGAGGTCCGAGACCTTCGCACCTA
  CATCATGCCTGCCCACCTCCGCACGGGTGTGGATGACGTCAAGGGTATCTTCTTTGCCTGGGCCCAGAAG
  ATAGAGGTGCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209077 representing NM_031526
  Red=Cloning site Green=Tags(s)
  
  MDRGSDPKHTAGMDSDSDPGQAEVALAYQRFEPRAYLRNNYAPPRGDLSNPDGVGPWKLRCMAQVFATGE
  VSGQVLIDIGSGPTIYQLLSACAHFEDITMTDFLEVNRQELGLWLREEPGAFDWSVYSQHVCLIEDKGES
  WQEKERQLRARVKRVLPIDVHKPQPLGASGLAPLPADALVSAFCLEAVSPDLPSFRQALYHITTLLRPGG
  NLLFIGALEESWYLAGEARLSVVPVSEEEVREALVCSGYEVRDLRTYIMPAHLRTGVDDVKGIFFAWAQK
  IEVQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031526
• ORF Size: 855 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031526.1, NP_113714.1
• RefSeq Size: 945 bp
• RefSeq ORF: 858 bp
• Locus ID: 24661
• UniProt ID: P10937
• Cytogenetics: 10q31
• MW: 32.1 kDa
• Gene Summary: catalyzes the conversion of norepinephrine to epinephrine in catecholamine biosynthesis [RGD, Feb 2006]