Pdcd7 (NM_001108768) Rat Tagged ORF Clone

CAT#: RR209030

Pdcd7 (Myc-DDK-tagged ORF) - Rat programmed cell death 7 (Pdcd7), (10 ug)

Product Images

Circular map for RR209030

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdcd7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209030 representing NM_001108768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGGCGCGACGGAGGCTGGAGAGGGTCCGGCGCCGCCGGCTGCGGCTTCGCGAGAGGGCCCGGG
  AACGTGAGACTGAGCGGGAGGCGGAGGCCGCGCAGGCTACGGAACGGGAGCAGGAGATTGACCGCTGGAG
  GATGAAGTGCGTGCAGGAAGTGGAGGAAAAGAAGCGGGAACAAGAACTCAAGGCAGCTGCTGATGGGGTC
  TTAGCTGAAGTAAGGAAAAAACAATCAGACACCAAAAGAATGGTGGACATTCTGCGTGCGTTGGAGAAAT
  TGAGAAAGCTGAGAAAAGAAGCTGCAGCAAGGAAAGGGGTCTGTCCTCCTGCGGCAGCAGATGAGACCTT
  TGAGCACCACCTTCAGCGGCTGAGGAAGCTCATTAAAAAGCGCTCTGAATTGTATGAAGCTGAAGAGAGA
  GCCCTCCGAGTTATGCTAGAAGGAGAACAGGAGGAAGAGAGGAGAAGAGAACTGGAAAAGAAACAGAGAA
  AAGAGAAAGAGAAACTTCTACTTCAGAAACGTGAAATCGAGTCCAAGTTATTCGGGGATCCAGATGAGTT
  CCCACTCGCTCACCTCTTGGAGCCGTTCCGTCAGTATTACCTCCAGGCTGAGCACTCCTTGCCTGCGCTC
  ATTCAAATAAGGCATGATTGGGATCAGTACCTGGTGCCGTCTGATCATCCTAAAGGCAATTCCATTCCCC
  AAGGATGGGTCCTTCCTCCGCTCCCCAGCAACGACATCTGGGCAACTGCTGTTCAGCTGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209030 representing NM_001108768
  Red=Cloning site Green=Tags(s)
  
  MGEARRRLERVRRRRLRLRERARERETEREAEAAQATEREQEIDRWRMKCVQEVEEKKREQELKAAADGV
  LAEVRKKQSDTKRMVDILRALEKLRKLRKEAAARKGVCPPAAADETFEHHLQRLRKLIKKRSELYEAEER
  ALRVMLEGEQEEERRRELEKKQRKEKEKLLLQKREIESKLFGDPDEFPLAHLLEPFRQYYLQAEHSLPAL
  IQIRHDWDQYLVPSDHPKGNSIPQGWVLPPLPSNDIWATAVQLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108768
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108768.1, NP_001102238.1
• RefSeq Size: 2255 bp
• RefSeq ORF: 765 bp
• Locus ID: 363082
• Cytogenetics: 8q24
• MW: 30.4 kDa