Foxa3 (NM_017077) Rat Tagged ORF Clone

CAT#: RR209012

Foxa3 (Myc-DDK-tagged ORF) - Rat forkhead box A3 (Foxa3), (10 ug)

Product Images

Circular map for RR209012

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxa3
• Synonyms: Hnf3g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209012 representing NM_017077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTCAGTGAAGATGGAGGCCCATGACCTGGCCGAGTGGAGCTACTACCCGGAGGCGGGCGAGG
  TGTATTCTCCAGTGAATCCTGTGCCCACCATGGCCCCTCTCAACTCCTACATGTCCTTGAACCCTCTTAG
  CTCTCCCTACCCTCCCGGAGGGCTTCAGGCCTCCCCTCTGCCTACAGGACCCCTGGCACCCCCAGCCCCC
  ACCGCTCCCCTGGGGCCCACCTTCCCAGGCCTGGGCGCAGGCAGCGGCACCGGAGGCAGTGCTTCCGGGT
  ATGGCGCCCCAGGGCCCGGGCTTGTACACGGAAAGGAAATGGCCAAGGGGTACCGGCGGCCACTGACTCA
  TGCCAAACCACCTTATTCCTACATCTCTCTCATCACCATGGCTATCCAGCAGGCACCAGGCAAGATGCTG
  ACCCTGAGTGAAATCTACCAATGGATCATGGACCTCTTCCCGTACTACCGGGAGAACCAGCAACGTTGGC
  AGAACTCCATCCGGCACTCGCTGTCCTTCAATGACTGCTTCGTCAAGGTGGCACGCTCCCCAGACAAACC
  GGGCAAAGGCTCCTACTGGGCCTTGCATCCCAGCTCTGGGAACATGTTTGAGAACGGCTGCTATCTCCGC
  CGTCAGAAGCGCTTCAAGCTGGAGGAGAAGGCAAAGAAAGGAAACAGTGCCACATCCGCCACCAGGAATG
  GAACAGTGGGGTCAGCCACCTCTGCCACCACTACAGCTGCCACTGCAGTCACTTCCCCGGCTCAGCCCCA
  GCCTACGCCGCCGTCTGAGCCCGAGGCCCAGAGTGGGGAAGATGTGGGGGGTCTGGACTGCGCCTCGCCT
  CCTTCATCCGCACCCTATTTCACTGGCCTGGAGCTCCCAGGGGAACTGAAGTTGGATGCGCCCTACAACT
  TCAACCACCCTTTCTCTATCAACAACCTGATGTCAGAACAGACATCAACACCTTCCAAACTAGATGTGGG
  GTTTGGGGGCTACGGTGCTGAGAGTGGGGAACCTGGGGTCTACTACCAGAGCCTCTATTCCCGCTCTCTG
  CTCAATGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209012 representing NM_017077
  Red=Cloning site Green=Tags(s)
  
  MLGSVKMEAHDLAEWSYYPEAGEVYSPVNPVPTMAPLNSYMSLNPLSSPYPPGGLQASPLPTGPLAPPAP
  TAPLGPTFPGLGAGSGTGGSASGYGAPGPGLVHGKEMAKGYRRPLTHAKPPYSYISLITMAIQQAPGKML
  TLSEIYQWIMDLFPYYRENQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWALHPSSGNMFENGCYLR
  RQKRFKLEEKAKKGNSATSATRNGTVGSATSATTTAATAVTSPAQPQPTPPSEPEAQSGEDVGGLDCASP
  PSSAPYFTGLELPGELKLDAPYNFNHPFSINNLMSEQTSTPSKLDVGFGGYGAESGEPGVYYQSLYSRSL
  LNAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017077
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017077.2, NP_058773.1
• RefSeq Size: 1496 bp
• RefSeq ORF: 1065 bp
• Locus ID: 25100
• UniProt ID: P32183
• Cytogenetics: 1q21
• MW: 37.7 kDa
• Gene Summary: member of the Forkhead box a (Foxa) transcription factor family; binds rat proglucagon gene G2 promoter element [RGD, Feb 2006]