Slc8a2 (NM_078619) Rat Tagged ORF Clone

CAT#: RR208988

Slc8a2 (Myc-DDK-tagged ORF) - Rat solute carrier family 8 (sodium/calcium exchanger), member 2 (Slc8a2), (10 ug)

Product Images

Circular map for RR208988

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc8a2
• Synonyms: Ncx2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208988 representing NM_078619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCTTGGCTTTGGTAGGGGTTGCCCTCCTTCTGGGGGCACCCCACTGCTTGGGGGAAGCTACTC
  CGACCCCATCCCTGCCACCTCCCCCAGCTAATGACAGCGATGCCAGCCCAGGCGGCTGCCAAGGTTCCTA
  CCGCTGCCAACCAGGGGTGCTGCTGCCTGTGTGGGAACCCGACGACCCATCACTGGGGGACAAGGCTGCA
  CGGGCCGTGGTGTACTTTGTGGCCATGGTCTACATGTTCTTGGGTCTGTCTATCATTGCTGATCGTTTTA
  TGGCATCCATTGAGGTCATCACATCCAAGGAGAAAGAGATCACCATCACCAAGGCAAATGGGGAGACCAG
  CGTGGGCACTGTACGCATCTGGAATGAAACGGTGTCCAACCTTACACTCATGGCCCTGGGCTCCTCAGCA
  CCTGAGATTCTGCTGTCTGTCATCGAGGTCTGTGGCCACAACTTCCAGGCGGGTGAGCTAGGCCCAGGCA
  CCATCGTGGGCAGTGCCGCCTTCAACATGTTTGTGGTCATTGCTGTGTGTGTGTATGTCATCCCGGCTGG
  TGAGAGCCGTAAGATCAAGCACTTAAGGGTCTTCTTCGTCACAGCCTCTTGGAGCATCTTTGCCTATGTC
  TGGCTTTATCTCATTCTAGCAGTTTTCTCTCCAGGCGTGGTCCAGGTGTGGGAGGCACTGCTCACGCTGG
  TCTTCTTCCCGGTGTGTGTGGTGTTCGCCTGGATGGCGGACAAGCGACTGCTCTTCTACAAGTACGTGTA
  CAAGCGCTATCGCACCGACCCTCGCAGCGGAATCATCATCGGGGCAGAGGGAGACCCGCCCAAGAGCATC
  GAGCTGGATGGCACATTCGTGGGCACTGAGGTCCCAGGCGAGCTGGGTGCATTGGGCACAGGTCCCGCTG
  AGGCGCGTGAGCTGGACGCCAGCCGGCGCGAGGTCATCCAGATTCTTAAGGACTTAAAGCAGAAGCACCC
  GGATAAGGACCTGGAGCAGCTGGTGGGCATCGCCAAGTACTATGCACTGCTGCACCAGCAGAAGAGCCGT
  GCCTTCTACCGCATCCAGGCCACGCGGCTGATGACAGGTGCGGGCAACGTGCTGCGCCGACACGCTGCGG
  ATGCTGCCCGCAGGCCTGGGGCCAACGATGGTGCCCCCGATGATGAGGACGATGGTGCCAGCCGCATCTT
  CTTTGAGCCCAGCCTCTACCACTGCCTGGAGAACTGCGGGTCAGTGCTGCTGTCGGTGGCTTGCCAGGGT
  GGTGAGGGCAACAGCACCTTCTACGTGGATTACCGCACGGAGGATGGCTCTGCAAAGGCAGGCTCCGATT
  ATGAGTACAGCGAGGGCACGCTGGTGTTCAAGCCCGGGGAGACGCAGAAGGAGCTGCGCATCGGCATCAT
  CGACGACGACATCTTCGAGGAGGACGAGCACTTCTTCGTGAGGCTGCTGAACCTGCGTGTGGGCGATGCT
  CAGGGCATGTTCGAGCCCGACGGCGGTGGGCGGCCCAAGGGGCGGCTGGTGGCGCCACTGCTGGCCACCG
  TCACCATTCTGGACGACGACCACGCGGGCATCTTCTCCTTCCAGGACCGCCTGCTGCATGTGAGCGAGTG
  CATGGGCACCGTGGATGTGCGTGTGGTGCGCAGCTCTGGCGCACGTGGCACCGTACGCCTTCCCTACCGC
  ACAGTGGACGGCACGGCTCGTGGTGGTGGTGTGCACTACGAGGATGCTTGTGGAGAGCTGGAGTTCGGCG
  ATGACGAGACCATGAAGACTCTTCAGGTCAAGATAGTGGACGACGAAGAGTATGAGAAGAAGGACAACTT
  CTTCATCGAGCTGGGCCAGCCCCAGTGGCTTAAGCGGGGCATCTCAGCTCTGCTACTCAACCAAGGGGAT
  GGAGACAGGAAGCTGACTGCAGAGGAGGAGGAGGCCCAGAGGATAGCAGAGATGGGCAAGCCAGTTCTTG
  GGGAGAACTGTCGCCTCGAGGTCATCATCGAGGAGTCTTATGACTTTAAGAATACGGTGGATAAACTCAT
  AAAGAAAACAAACCTGGCCTTGGTGATTGGGACCCACTCATGGAGGGAGCAGTTTTTAGAGGCAGTTACA
  GTGAGCGCAGGGGACGAGGAGGAGGATGAGGATGGGTCTCGTGAGGAGCGGCTGCCATCCTGCTTTGACT
  ACGTGATGCACTTCCTGACGGTGTTCTGGAAAGTTCTGTTCGCCTGCCTTCCACCCACGGAGTACTGCCA
  TGGCTGGGCCTGCTTTGGTGTCTGCATCCTGGTCATTGGTCTGCTCACTGCCCTCATCGGAGACCTGGCC
  TCACACTTTGGGTGCACCGTGGGCCTCAAGGACTCAGTCAACGCCGTGGTCTTCGTGGCTCTGGGCACCT
  CCATCCCTGACACGTTTGCCAGCAAGGTGGCCGCGCTGCAGGACCAGTGCGCCGACGCGTCCATCGGTAA
  CGTGACCGGCTCCAATGCGGTGAACGTGTTCCTGGGCCTGGGTGTGGCCTGGTCGGTGGCCGCAGTGTAC
  TGGGCGGTGCAGGGTCGCCCCTTCGAGGTGCGTACAGGCACGCTGGCCTTCTCGGTCACACTGTTCACCG
  TCTTCGCCTTCGTGGGCATCGCAGTGCTCTTGTACCGGCGCCGGCCACACATCGGCGGCGAGCTGGGCGG
  CCCGCGGGGACCCAAGCTAGCCACCACCGCTCTCTTCCTGGGCCTCTGGTTCCTCTACATTCTCTTCGCC
  AGCCTGGAGGCTTATTGCCACATTCGGGGCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208988 representing NM_078619
  Red=Cloning site Green=Tags(s)
  
  MAPLALVGVALLLGAPHCLGEATPTPSLPPPPANDSDASPGGCQGSYRCQPGVLLPVWEPDDPSLGDKAA
  RAVVYFVAMVYMFLGLSIIADRFMASIEVITSKEKEITITKANGETSVGTVRIWNETVSNLTLMALGSSA
  PEILLSVIEVCGHNFQAGELGPGTIVGSAAFNMFVVIAVCVYVIPAGESRKIKHLRVFFVTASWSIFAYV
  WLYLILAVFSPGVVQVWEALLTLVFFPVCVVFAWMADKRLLFYKYVYKRYRTDPRSGIIIGAEGDPPKSI
  ELDGTFVGTEVPGELGALGTGPAEARELDASRREVIQILKDLKQKHPDKDLEQLVGIAKYYALLHQQKSR
  AFYRIQATRLMTGAGNVLRRHAADAARRPGANDGAPDDEDDGASRIFFEPSLYHCLENCGSVLLSVACQG
  GEGNSTFYVDYRTEDGSAKAGSDYEYSEGTLVFKPGETQKELRIGIIDDDIFEEDEHFFVRLLNLRVGDA
  QGMFEPDGGGRPKGRLVAPLLATVTILDDDHAGIFSFQDRLLHVSECMGTVDVRVVRSSGARGTVRLPYR
  TVDGTARGGGVHYEDACGELEFGDDETMKTLQVKIVDDEEYEKKDNFFIELGQPQWLKRGISALLLNQGD
  GDRKLTAEEEEAQRIAEMGKPVLGENCRLEVIIEESYDFKNTVDKLIKKTNLALVIGTHSWREQFLEAVT
  VSAGDEEEDEDGSREERLPSCFDYVMHFLTVFWKVLFACLPPTEYCHGWACFGVCILVIGLLTALIGDLA
  SHFGCTVGLKDSVNAVVFVALGTSIPDTFASKVAALQDQCADASIGNVTGSNAVNVFLGLGVAWSVAAVY
  WAVQGRPFEVRTGTLAFSVTLFTVFAFVGIAVLLYRRRPHIGGELGGPRGPKLATTALFLGLWFLYILFA
  SLEAYCHIRGF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078619
• ORF Size: 2763 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078619.1, NP_511174.1
• RefSeq Size: 3004 bp
• RefSeq ORF: 2766 bp
• Locus ID: 140447
• UniProt ID: P48768
• Cytogenetics: 1q21
• MW: 100.5 kDa
• Gene Summary: isoform 2 of sodium/calcium exchanger; regulates intracellular calcium levels and maintains calcium homeostasis; may be important for the control of synaptic plasticity [RGD, Feb 2006]