Top2a (NM_022183) Rat Tagged ORF Clone

CAT#: RR208980

Top2a (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) II alpha (Top2a), (10 ug)

Product Images

Circular map for RR208980

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Top2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208980 representing NM_022183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGTCACCATTGCAGCCTGTAAATGAAAATATGCTACTCAACAAAAAGAAGAATGAAGATGGCA
  AGAAAAGACTGTCCGTTGAAAGAATCTATCAGAAAAAAACCCAACTGGAACATATACTGCTCCGCCCAGA
  TACCTACATCGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGTATTAAC
  TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAAATTCTAGTTAATGCTGCTGATA
  ACAAACAAAGGGACCCAAAAATGTCTTGCATTAGAGTCACAATGATGCGAAATAATTTAATTAGTATATG
  GAATAATGGAAAGGGAATTCCTGTTGTTGAACACAAAGTGGAGAAAATGTATGTCCCAGCTCTCATATTT
  GGACAGCTCCTGACCTCCAGTAACTACGATGATGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCTATG
  GAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGGGAATACAAGAAAAT
  GTTCAAACAGACATGGATGGATAACATGGGGAGAGCTGGTGACATGGAGCTCAAGCCCTTTAGCGGAGAG
  GATTACACGTGTATTACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATATTG
  TCGCACTGATGGTCAGAAGAGCATACGATATTGCTGGTTCCACCAAAGATGTAAAAGTCTTTCTTAATGG
  AAACAGGCTGCCAGTCAAAGGGTTCCGTAGTTACGTGGATATGTATTTGAAGGATAAGGTTGATGAAACT
  GGGAACGCTCTGAAGGTGGTGCATGAACAAGTAAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCGAGA
  AAGGCTTTCAGCAGATTAGCTTCGTCAACAGCATCGCTACTTCTAAGGGTGGCAGACATGTCGATTATGT
  AGCCGATCAGATTGTGAGTAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGAGGTGTTGCAGTGAAG
  GCCGATCAGGTGAAGAATCACATGTGGATTTTTGGAAATGCGGTAATTGAAAACCCAACCTTTGATTCTC
  AAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACTTGTCAATTAAGTGAAAAATTCAT
  CAAAGCTGCAATTGGTTGTGGTATTGTCGAAAGCATACTAAACTGGGTGAAGTTTAAGGCCCAAATCCAA
  TTAAACAAGAAGTGTTCAGCTGTAAAACATAACAGGATCAAGGGAATCCCCAAACTTGACGACGCCAATG
  ACGCAGGGAGCCGAAACTCTGCTGAATGCACACTTATCCTGACTGAAGGGGACTCAGCCAAAACTCTGGC
  AGTCTCAGGACTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTCAGAGGAAAAATACTCAAT
  GTGCGAGAAGCTTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGGGGC
  TTCAGTACAAGAAAAACTATGAGGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGATTAT
  GACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGGCCA
  TCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCTATTGTAAAGGTATCTAAAAACAAGCAAG
  AAATAGCATTCTACAGTCTTCCCGAGTTTGAAGAATGGAAGAGTACAAATCCAAATCACAAAAAATGGAA
  AGTCAAGTACTACAAAGGTTTGGGCACGAGCACATCAAAGGAAGCTAAGGAGTATTTTGCAAATATGAAA
  CGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGACGATGCTGCGATCAGCCTGGCCTTTAGTAAAA
  AACAGGTCGATGATCGAAAGGAATGGTTAACTAATTTCATGGAAGATAGAAGACAGCGGAAGTTACTTGG
  CCTTCCTGAGGATTATTTGTATGGACAAACCACTATGTACCTGACCTATAATGATTTCATTAACAAGGAA
  CTTATCCTCTTTTCAAACTCTGATAATGAAAGATCTATCCCATCTATGGTAGATGGTTTGAAACCAGGTC
  AGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAGGTGAAAGTTGCCCAGTTAGCTGG
  GTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCGCTGATGATGACCATTATCAATTTGGCT
  CAGAATTTTGTGGGTAGCAATAATTTGAACCTTCTACAGCCTATTGGTCAGTTTGGTACCAGGCTGCATG
  GTGGCAAGGACTCAGCCAGCCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTATTTCC
  ATCAAAAGATGATCACACGTTACGGTTTCTGTATGACGACAACCAGCGTGTTGAGCCTGAATGGTACATC
  CCTATTATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCCCCA
  ACTTTGATGTTCGTGAAGTCGTAAATAATATCAGGCGGCTGTTGGATGGAGAAGAGCCTCTGCCCATGCT
  CCCAAGTTATAAGAATTACAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGAGAA
  GTAGCTATTCTGAATTCCACAACCATTGAGATCACTGAGCTTCCCATCAGAACATGGACCCAGACATATA
  AGGAACAGGTTCTAGAACCTATGTTGAATGGCACCGAGAAGACACCCCCTCTAATAACAGACTATAGGGA
  ATACCACACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAATTGGCAGAGGCAGAGAGA
  GTGGGACTACACAAAGTCTTCAAACTCCAGACTAGTCTCACTTGCAACTCTATGGTACTTTTTGACCATG
  TTGGTTGTTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAAATA
  TTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAAGCT
  CGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAGAAAGAATTAATTAAAG
  TTCTGATTCAGAGAGGCTATGACTCCGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTCCCAGA
  AGAAGAGGAAAATGAAGAGAATGAAGAGAGCGAGAGTGAAAGCACGAGCCCTGCAGCGGAGTCTGGACCG
  ACCTTCAACTACCTTCTTGATATGCCCCTGTGGTATCTAACCAAGGAGAAGAAGGACGAGCTGTGCAAGC
  AAAGGGACGAGAAGGAACAAGAGCTCAACACGTTAAAGAAAAAGACTCCATCGGATTTGTGGAAGGAAGA
  CCTGGCTGCTTTTGTGGAAGAACTGGAGGTTGTTGAAGCCAAGGAAAAACAGGATGAACAAGTAGGACTT
  CCTGGAAAGGGGGTGAAAGCGAAGGGGAAGAAAGCGCAGATATCTGAAGTCCTGCCTTCTCCTGTGGGGA
  AAAGGGTCATCCCGCAGGTGACTATGGAGATGAGAGCTGAGGCAGAGAAGAAAATCAGGAGGAAAATTAA
  GAGCGAGAATGTTGAGGGCACCCCTGCTGAGGATGGTGCTGAGCCAGGACTCCGGCAGAGACTAGAGAAG
  AGGCAGAAGAGAGAGCCAGGTACCAGGGCAAAGAAGCAAACTACATTGCCATTTAAGCCTATCAAAAAAG
  CCCAAAAGCAAAACCCTTGGTCTGATTCTGAATCAGATATGAGCAGCAACGAAAGCAATTTTGATGTCCC
  TCCACGAGAGAAAGAGCCAAGAATTGCTGCAACAAAAGCCAAATTCACGGCGGATTTAGACTCTGATGAC
  GATTTCTCAGGCTTGGATGAGAAGGATGAGGATGAAGATTTCTTCCCATTAGATGATACTCCACCTAAGA
  CCAAAATGCCCCCAAAAAATACTAAAAAAGCACTGAAGCCACAGAAGAGCTCCACATCAGTTGATCTTGA
  AAGTGATGGGAAAGACAGTGTACCAGCTTCTCCAGGCGCTTCTGCTGCTGACGTCCCAGCTGAGACTGAA
  CCCTCCAAGCCGTCCTCCAAACAGACAGTGGGAGTGAAGAGGACAATAACCAAAGGTCAGTCTTTAACCT
  CCACTGCTGGCACCAAAAAGAGAGCTGTACCAAAGGAGACCAAGTCAGATTCAGCCTTGAATGCTCATGT
  CTCCAAAAAGCCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAGAGGATGCCATCTTCTTCTGACAGCTCG
  GACTCTGAGTTTGAGAAGGCGATTTCTAAAGGTGCCACAAGCAAGAAACTCAAGGGAGAGGAGCGGGATT
  TCCATGTGGACTTAGATGACACTGTGGCTCCTCGAGCAAAATCCGGACGAGCAAGGAAGCCGATTAAGTA
  CCTGGAGGAGTCGGATGACGACCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208980 representing NM_022183
  Red=Cloning site Green=Tags(s)
  
  MEVSPLQPVNENMLLNKKKNEDGKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGIN
  YREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTMMRNNLISIWNNGKGIPVVEHKVEKMYVPALIF
  GQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGE
  DYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVDET
  GNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVK
  ADQVKNHMWIFGNAVIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQ
  LNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILN
  VREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWP
  SLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMK
  RHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKE
  LILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLA
  QNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYI
  PIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGE
  VAILNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAER
  VGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQA
  RFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPEEEENEENEESESESTSPAAESGP
  TFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGL
  PGKGVKAKGKKAQISEVLPSPVGKRVIPQVTMEMRAEAEKKIRRKIKSENVEGTPAEDGAEPGLRQRLEK
  RQKREPGTRAKKQTTLPFKPIKKAQKQNPWSDSESDMSSNESNFDVPPREKEPRIAATKAKFTADLDSDD
  DFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSVPASPGASAADVPAETE
  PSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKAKNSRKRMPSSSDSS
  DSEFEKAISKGATSKKLKGEERDFHVDLDDTVAPRAKSGRARKPIKYLEESDDDLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022183
• ORF Size: 4578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022183.2, NP_071519.2
• RefSeq Size: 4987 bp
• RefSeq ORF: 4581 bp
• Locus ID: 360243
• UniProt ID: P41516
• Cytogenetics: 10q31
• MW: 173.2 kDa
• Gene Summary: DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006]