Ap2a1 (NM_001107511) Rat Tagged ORF Clone

CAT#: RR208798

Ap2a1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap2a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208798 representing NM_001107511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCTGTATCCAAAGGCGATGGCATGCGTGGGCTTGCCGTGTTCATCTCCGACATCCGGAACTGCA
  AGAGCAAAGAGGCTGAGATCAAGAGGATCAACAAGGAACTGGCCAACATCCGCTCCAAGTTCAAAGGGGA
  CAAGGCCTTGGATGGCTACAGTAAAAAGAAGTATGTGTGTAAGCTGCTCTTCATCTTCCTGCTTGGCCAT
  GACATTGACTTTGGACACATGGAGGCTGTGAACCTGCTAAGCTCCAACAAGTACACAGAGAAGCAGATAG
  GGTACCTGTTCATCTCAGTCCTGGTGAACTCGAACTCAGAGCTGATCCGGCTCATTAACAACGCTATCAA
  GAACGACCTGGCCAGTCGCAACCCCACCTTCATGTGCCTGGCTTTGCACTGTATTGCCAACGTGGGCAGC
  CGTGAGATGGGCGAGGCCTTTGCTGCCGACATTCCCCGAATCCTGGTGGCCGGAGACAGCATGGATAGTG
  TGAAGCAGAGTGCAGCCCTATGCCTACTGCGTCTCTACAAGGCCTCCCCCGACTTGGTGCCCATGGGCGA
  GTGGACGGCCCGGGTGGTGCATTTGCTCAATGATCAGCACATGGGAGTGGTCACAGCCGCTGTCAGTCTC
  ATCACCTGCCTGTGCAAGAAGAATCCAGATGACTTCAAGACCTGTGTCTCCCTGGCTGTGTCTCGCCTAA
  GCCGGATTGTCTCCTCAGCCTCCACTGACCTCCAGGACTACACTTACTACTTCGTTCCTGCACCCTGGCT
  CTCTGTGAAGTTACTGCGGTTGCTACAGTGTTACCCACCGCCAGAGGATGCGGCTGTGAAAGGGCGGTTA
  GTGGAGTGTCTTGAGACTGTGCTCAACAAGGCCCAGGAGCCTCCCAAGTCCAAGAAGGTGCAGCACTCCA
  ATGCCAAGAACGCCATCCTGTTTGAGACTATTAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT
  GGTCCGTGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGAGAGACGAACCTGCGCTACCTGGCTCTG
  GAGAGCATGTGCACCCTGGCCAGCTCCGAGTTCTCCCACGAGGCTGTCAAGACCCACATTGATACTGTTA
  TTAATGCCCTCAAGACTGAGCGGGACGTGAGTGTGAGGCAGCGGGCAGCTGATCTGCTGTATGCCATGTG
  TGACCGGAGCAATGCCAAGCAGATTGTATCAGAGATGCTGCGGTACCTAGAGACGGCTGACTATGCCATC
  CGAGAGGAGATTGTGCTGAAGGTGGCCATCTTGGCTGAGAAGTATGCAGTGGACTACAGCTGGTATGTGG
  ACACCATCCTCAACCTCATCCGCATTGCAGGCGACTATGTGAGCGAGGAGGTGTGGTACCGAGTGCTGCA
  GATCGTCACCAACCGTGATGATGTCCAGGGTTATGCTGCCAAGACAGTGTTTGAGGCCCTCCAGGCCCCA
  GCCTGTCATGAGAACATGGTGAAGGTCGGTGGCTACATCCTTGGGGAGTTTGGGAACTTGATTGCTGGGG
  ACCCACGCTCCAGCCCACCTGTGCAGTTCTCACTGCTGCACTCCAAGTTCCACCTGTGCAGTGTGGCCAC
  TCGTGCCTTGCTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCTGAGACCAAGGCCACCATCCAA
  GGTGTTCTTCGTGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTACAGCAGCGGGCAGTGGAGTACC
  TCACCCTCAGCTCTGTAGCTAGCACCGATGTTTTGGCTACTGTGCTAGAAGAAATGCCCCCATTTCCTGA
  GCGGGAGTCATCCATTTTGGCTAAGCTGAAGCGTAAGAAGGGCCCTGGGGCAGCCAGTGCCCTGGATGAC
  AGCCGCAGGGACACCAGCAGCAATGACATCAATGGGGGTGTGGAGCCCACCCCCAGCAATGTGTCAACCC
  CCTCACCCTCCGCGGACCTCTTGGGGCTGCGGGCAGCCCCTCCCCCTGCTGCACCCCCAGCTCCTGTAGG
  CGGGAACCTCCTGGTGGATGTTTTCTCTGACGGCCCCACTGCACAGCCCAGCCTGGGTCCCACCCCTGAG
  GAGGCCTTCCTCAGCGAGCTGGAGCCCCCTGCCCCTGAGAGCCCCATGACTTTGTTGGCTGACCCAGCTC
  CAGCTGCTGACCCAGGTCCTGAGGACATAGGTCCTCCCATTCCAGAAGCAGATGAACTGCTGAATAAGTT
  CGTGTGTAAGAATAGTGGGGTCTTGTTTGAGAACCAGCTGCTGCAGATCGGAGTCAAGTCTGAGTTCCGG
  CAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACTTCTGTGCAGTTCCAGAGCTTCTTGCCCA
  CCGTGGTCCACCCTGGGGACCTCCAGACTCATCTGGCGGTGCAGACCAAGCGTGTAGCTGCACAAGTGGA
  CGGTGGCGCACAGGTGCAGCAAGTACTCAACATTGAGTGTCTGCGAGACTTCTTGACGCCACCACTGCTG
  TCGGTGCGCTTCCGGTACGGTGGCACCGCCCAGTCCCTCACTCTGAAGCTCCCAGTGACCATCAACAAAT
  TCTTCCAGCCCACAGAGATGGCCGCCCAGGACTTTTTCCAGCGCTGGAAGCAGCTGAGCCTCCCCCTGCA
  GGAGGCACAGAAAATCTTCAAAGCCAACCACCCCATGGATGCTGAAGTCACAAAGGCCAAGCTTCTGGGG
  TTTGGCTCTGCTCTTCTGGACAATGTGGATCCCAACCCTGAGAACTTTGTGGGTGCTGGAATCATCCAGA
  CGAAAGCCCTGCAGGTGGGGTGTCTGCTTCGACTGGAGCCCAATGCCCAGGCCCAAATGTACCGCCTAAC
  CCTGCGCACCAGCAAAGAGCCTGTGTCCCGTCACTTATGTGAGCTGCTGGCCCAGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208798 representing NM_001107511
  Red=Cloning site Green=Tags(s)
  
  MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
  DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS
  REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL
  ITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL
  VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL
  ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
  REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP
  ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ
  GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDD
  SRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPE
  EAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFR
  QNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTHLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLL
  SVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLG
  FGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107511
• ORF Size: 2931 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107511.2, NP_001100981.1
• RefSeq Size: 2934 bp
• RefSeq ORF: 2934 bp
• Locus ID: 308578
• Cytogenetics: 1q22
• MW: 108.1 kDa