Dnajb6 (NM_001013209) Rat Tagged ORF Clone

CAT#: RR208753

Dnajb6 (Myc-DDK-tagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily B, member 6 (Dnajb6), (10 ug)

Product Images

Circular map for RR208753

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnajb6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208753 representing NM_001013209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATTACTATGAAGTGCTGGGCGTGCAGAGACATGCCTCACCTGAGGACATTAAAAAGGCGTATC
  GAAAACAGGCACTCAAATGGCACCCAGACAAAAATCCTGAAAATAAAGAAGAAGCGGAGCGGAAATTCAA
  ACAAGTAGCTGAGGCATATGAAGTGTTATCGGATGCTAAAAAGCGGGACATCTATGACAAATATGGCAAA
  GAAGGATTAAATGGTGGTGGAGGAGGAGGTGGAAGTCATTTTGACAGTCCATTTGAGTTTGGCTTCACAT
  TCCGGAACCCAGATGATGTCTTCAGGGAATTTTTTGGTGGAAGGGACCCATTTTCATTTGACTTCTTTGA
  AGATCCATTTGATGACTTTTTTGGAAACCGAAGGGGTCCCCGAGGAAGTAGAAGCCGAGGTGCTGGCTCC
  TTTTTCTCCGCCTTCAGTGGATTTCCTTCTTTTGGAAGTGGATTTCCTGCTTTTGATACAGGCTTTACTC
  CATTCGGGTCACTAGGTCATGGGGGTCTCACTTCATTTTCTTCAGCGTCATTTGGCGGCAGTGGAATGGG
  CAACTTCAAATCAATATCAACTTCAACTAAAATAGTTAATGGCAAAAAAATCACTACAAAGAGAATTGTG
  GAGAACGGTCAGGAAAGAGTAGAAGTTGAAGAAGACGGACAGTTAAAGTCCCTGACGATAAATGGTGTGG
  CCGACGAGAACGCCCTTGCAGAAGAATGCCGGCGGAGAGGCCAACCCACACCAGCTCTGGCCCCTGGCCC
  GGCCCCAGCACCAGCCCGGGTCCCCTCTCAGGCCCGACCCCCGACCCCAGCTCCCACCCCAGCTCCAGCC
  CAGACTCCAGCCCCTTCTGTCAGCACCCGCCCACAGAAGCCACCCAGGCCTGCCCCAACGGCCAAACTTG
  TGTCTAAAAGCAACTGGGAAGATGAAGAGCAGGACAGGCAGCGGGTTCCTGGCAACTGTGATGCCCCTAT
  GACCTCAGCAGGGCTCAAAGAAGGTGGCAAGAGGAAGAAGCAGAAGCAGAAAGAGGACTCGAAGAAGAAG
  AAGTCGACCAAAGGGAACCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208753 representing NM_001013209
  Red=Cloning site Green=Tags(s)
  
  MVDYYEVLGVQRHASPEDIKKAYRKQALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGK
  EGLNGGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFDDFFGNRRGPRGSRSRGAGS
  FFSAFSGFPSFGSGFPAFDTGFTPFGSLGHGGLTSFSSASFGGSGMGNFKSISTSTKIVNGKKITTKRIV
  ENGQERVEVEEDGQLKSLTINGVADENALAEECRRRGQPTPALAPGPAPAPARVPSQARPPTPAPTPAPA
  QTPAPSVSTRPQKPPRPAPTAKLVSKSNWEDEEQDRQRVPGNCDAPMTSAGLKEGGKRKKQKQKEDSKKK
  KSTKGNH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013209
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013209.1, NP_001013227.1
• RefSeq Size: 2474 bp
• RefSeq ORF: 1074 bp
• Locus ID: 362293
• UniProt ID: Q6AYU3
• Cytogenetics: 4q11
• MW: 38.9 kDa
• Gene Summary: Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).[UniProtKB/Swiss-Prot Function]