Dmrt3 (NM_001106358) Rat Tagged ORF Clone

CAT#: RR208538

Dmrt3 (Myc-DDK-tagged ORF) - Rat doublesex and mab-3 related transcription factor 3 (Dmrt3), (10 ug)

Product Images

Circular map for RR208538

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmrt3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208538 representing NM_001106358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCTACGGCTCCCCCTACCTGTACATGGGCGGCCCGGTGTCGCAGCCGCCGCGGGCGCCCTTGC
  AGCGGACGCCCAAGTGCGCGCGCTGCCGGAACCACGGGGTGCTATCCTGGCTTAAGGGCCACAAGCGCTA
  CTGTCGCTTCAAGGACTGCACCTGCGAGAAGTGCATCCTGATCATCGAGCGGCAGCGGGTGATGGCGGCG
  CAGGTGGCGCTGCGGCGGCAGCAGGCCAACGAGAGCCTGGAGAGCCTCATCCCGGACTCGCTGCGGGCTC
  TGCCAGGGCCTCCGCCGCCGGGGGACGCCGCGGCCACCGCCGCCACCGCCTCGCAGTCGTCCCCAGCCTC
  CCAGGCGTCCCAGCCGCCGGCGCCGCCTCGTCCCGCCACCGAGTTGGCCGCGGCCGCTGCGCTGCGCTGG
  GTCGCCGAGCCCCAGCCCGGGACGCTGCCAGCGCAGATTGCTAAGCCAGATTTGACTGAAGACCGACTTG
  GGGACAGCAGTTCAGCAGACAATGCTGCGGAGTCCTTCAGTGACAAAGACACCGACCAGAGGAGCTCCCC
  AGATGTGGTCAAAAGCAAGAACTGCTTCACTCCGGAGAGCCCCGAGATCGTGTCCGTGGATGAAGGTGGG
  TACGCCGTGCAGAAAAACGGGGGCAACCCGGAGAGCTGCCCTGACAGCCCCAAGTACCACGCTGAGCAGA
  GCCATCTCCTGATCGAGGGCCCGTCAGGGACCGTCTCTCTGCCCTTCAGCTTGAAAGCCAACCGGCCACC
  CCTGGAAGTGTTGAAGAAAATCTTTCCCAACCAGAAGCCCACTGTACTGGAGCTCATCCTGAAAGGCTGC
  GGGGGCGACCTGGTCAGTGCCGTGGAGGTCCTGCTGTCCAGTCGGTCCTCCGCGGCTGGGGCGGAGCGGA
  CGGCCGAAGAGAGCCTGGTGCTGCCCTCCAGCGGCCATATCTTTGAACACACGCTGGGCTCCTACCCTAT
  CTCTTCCTCCAAGTGGTCCGTGGGCTCTGCCTTCCGAGTCCCTGACACCTTACGCTTTTCCGCGGACTCC
  AGCAACGTGGTCCCCAACCCCTTGGCCGTGCCCCTGCAACATCCTTTCCCCCAGCCACCCCGGTATCCGC
  TGATGCTGAGGAATACTTTGGCCAGAAACCAGTCGAGCCCCTTTTTGCCCAATGACGTCACCCTGTGGAA
  TACCATGACACTGCAGCAGCAGTACCAGCTGAGGTCCCAGTATGTCAGTCCATTTCCCAGTAACTCCACT
  AGCGTCTTCCGGAGCTCGCCCGTGCTCTCCTCTCGCACCACAGAAGACCCTCGGATCTCTATCCCCGACG
  ATGGGTGTCCAATCGTGGCAAAGCAGTCCATTTACACAGAGGATGACTATGATGAGAGGTCCGACTCCTC
  GGATTCTAGAATACTCAACACGTCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208538 representing NM_001106358
  Red=Cloning site Green=Tags(s)
  
  MNGYGSPYLYMGGPVSQPPRAPLQRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCEKCILIIERQRVMAA
  QVALRRQQANESLESLIPDSLRALPGPPPPGDAAATAATASQSSPASQASQPPAPPRPATELAAAAALRW
  VAEPQPGTLPAQIAKPDLTEDRLGDSSSADNAAESFSDKDTDQRSSPDVVKSKNCFTPESPEIVSVDEGG
  YAVQKNGGNPESCPDSPKYHAEQSHLLIEGPSGTVSLPFSLKANRPPLEVLKKIFPNQKPTVLELILKGC
  GGDLVSAVEVLLSSRSSAAGAERTAEESLVLPSSGHIFEHTLGSYPISSSKWSVGSAFRVPDTLRFSADS
  SNVVPNPLAVPLQHPFPQPPRYPLMLRNTLARNQSSPFLPNDVTLWNTMTLQQQYQLRSQYVSPFPSNST
  SVFRSSPVLSSRTTEDPRISIPDDGCPIVAKQSIYTEDDYDERSDSSDSRILNTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106358
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106358.1, NP_001099828.1
• RefSeq Size: 2394 bp
• RefSeq ORF: 1431 bp
• Locus ID: 293976
• UniProt ID: D4A218
• Cytogenetics: 1q51
• MW: 51.4 kDa
• Gene Summary: Probable transcription factor that plays a role in configuring the spinal circuits controlling stride in vertebrates. Involved in neuronal specification within a specific subdivision of spinal cord neurons and in the development of a coordinated locomotor network controlling limb movements. May regulate transcription during sexual development (By similarity).[UniProtKB/Swiss-Prot Function]