Ahsg (NM_012898) Rat Tagged ORF Clone

CAT#: RR208449

Ahsg (Myc-DDK-tagged ORF) - Rat alpha-2-HS-glycoprotein (Ahsg), (10 ug)

Product Images

Circular map for RR208449

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahsg
• Synonyms: Aa2-066; pp63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208449 representing NM_012898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCCCTGGTCCTGCTCCTTTGTTTTGCTCAGCTCTGGAGCTGCCAATCAGCTCCACAAGGTGCAG
  GGCTGGGTTTTCGAGAATTGGCTTGTGATGACCCGGAAACAGAGCATGTAGCTTTGATAGCCGTGCACTA
  CCTCAATAAACATCTTCTTCAGGGATTCAGGCAGATCTTGAATCAGATTGACAAAGTCAAGGTGTGGTCT
  CGGCGGCCCTTCGGACAGGTGTATGAGTTGGAAATTGACACGCTGGAGACAACCTGCCACGCTCTGGACC
  CAACCCCCTTAGCAAATTGTTCTGTGAGGCAACAGGCCGAGCATGCGGTGGAGGGAGACTGTGACTTCCA
  CATCCTGAAACAAGATGGCCAGTTCAGGGTGCTACACGCCCAGTGTCATTCCACACCAGACTCTGCAGAG
  GACGTTCGGAAGTTTTGCCCACGATGCCCAATCCTGATCCGGTTCAATGACACCAACGTGGTCCACACTG
  TCAAAACTGCCCTGGCTGCCTTCAATGCACAGAATAATGGAACCTATTTTAAACTGGTGGAGATTTCCCG
  AGCTCAAAATGTGCCTTTCCCGGTGTCTACTCTCGTGGAGTTTGTGATAGCTGCGACTGACTGTACTGGT
  CAGGAGGTCACAGATCCAGCCAAATGCAACCTGCTGGCAGAAAAGCAATATGGCTTCTGCAAGGCGACTC
  TCATCCACAGACTTGGTGGGGAAGAGGTTTCAGTGGCCTGCAAGTTATTCCAGACACAGCCCCAGCCAGC
  CAATGCCAACCCTGCAGGTCCTGCACCCACAGTGGGCCAAGCTGCGCCTGTAGCCCCACCAGCTGGCCCA
  CCTGAATCCGTGGTGGTAGGACCCGTGGCAGTTCCTCTAGGACTTCCAGACCACCGAACCCACCATGACC
  TACGGCATGCCTTCTCTCCTGTGGCTTCTGTGGAGTCGGCCTCAGGAGAAGTTCTTCACTCACCTAAGGT
  GGGCCAGCCTGGTGACGCTGGTGCTGCTGGCCCAGTGGCCCCCCTGTGCCCAGGGAGAGTCAGATACTTC
  AAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208449 representing NM_012898
  Red=Cloning site Green=Tags(s)
  
  MKSLVLLLCFAQLWSCQSAPQGAGLGFRELACDDPETEHVALIAVHYLNKHLLQGFRQILNQIDKVKVWS
  RRPFGQVYELEIDTLETTCHALDPTPLANCSVRQQAEHAVEGDCDFHILKQDGQFRVLHAQCHSTPDSAE
  DVRKFCPRCPILIRFNDTNVVHTVKTALAAFNAQNNGTYFKLVEISRAQNVPFPVSTLVEFVIAATDCTG
  QEVTDPAKCNLLAEKQYGFCKATLIHRLGGEEVSVACKLFQTQPQPANANPAGPAPTVGQAAPVAPPAGP
  PESVVVGPVAVPLGLPDHRTHHDLRHAFSPVASVESASGEVLHSPKVGQPGDAGAAGPVAPLCPGRVRYF
  KI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012898
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012898.4, NP_037030.1
• RefSeq Size: 1490 bp
• RefSeq ORF: 1059 bp
• Locus ID: 25373
• UniProt ID: P24090
• Cytogenetics: 11q23
• MW: 38 kDa
• Gene Summary: inhibits insulin receptor tyrosine kinase activity; may be involved in bone mineralization and immune response [RGD, Feb 2006]