Ufd1 (NM_053418) Rat Tagged ORF Clone

CAT#: RR208364

Ufd1l (Myc-DDK-tagged ORF) - Rat ubiquitin fusion degradation 1 like (yeast) (Ufd1l), (10 ug)

Product Images

Circular map for RR208364

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ufd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208364 representing NM_053418
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCTTTCAACATGTTTGACCACCCGATTCCCCGGGTCTTCCAGAACCGCTTCTCCACGCAGTACC
  GCTGCTTCTCTGTATCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGATAAT
  TATGCCACCCTCAGCCCTCGATCAACTCAGCCGGCTCAACATTACCTATCCTATGCTGTTTAAATTGACC
  AATAAGAATTCAGACCGAATGACACACTGTGGTGTACTGGAGTTTGTTGCTGATGAAGGCATCTGTTACC
  TCCCCCACTGGATGATGCAGAATTTGCTGTTGGAAGAAGGGGGCCTGGTTCAGGTGGAAAGTGTCAACCT
  TCAGGTGGCTACTTACTCTAAGTTCCAGCCTCAGAGCCCAGACTTCCTGGATATCACCAACCCCAAAGCA
  GTATTAGAAAATGCACTGAGGAACTTCGCCTGTCTGACGACTGGAGATGTGATCGCTATCAACTACAATG
  AGAAGATCTATGAACTGCGGGTGATGGAGACCAAACCGGACAAGGCCGTATCCATTATTGAATGTGACAT
  GAATGTGGATTTTGATGCTCCCTTGGGCTACAAAGAACCAGAAAGACCAGTACAGCATGAGGAGTCAATA
  GAGGGAGAAGCTGACCACAGTGGCTATGCTGGAGAAGTGGGCTTCCGTGCCTTCTCTGGTTCTGGGAATA
  GACTGGATGGAAAGAAAAAGGGGGTTGAACCCAGTCCCTCCCCAATCAAGCCTGGAGACATTAAAAGAGG
  AATTCCTAATTACGAATTTAAGCTTGGTAAGATCACTTTCATCAGAAATTCACGTCCAATGGTCAAAAAG
  GTTGAAGAGGATGAAGCTGGAGGCAGATTCGTTGCTTTCTCTGGAGAAGGACAGTCGCTGCGGAAGAAGG
  GAAGAAAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208364 representing NM_053418
  Red=Cloning site Green=Tags(s)
  
  MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLT
  NKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKA
  VLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERPVQHEESI
  EGEADHSGYAGEVGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPMVKK
  VEEDEAGGRFVAFSGEGQSLRKKGRKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053418
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053418.2, NP_445870.1
• RefSeq Size: 1802 bp
• RefSeq ORF: 924 bp
• Locus ID: 84478
• UniProt ID: Q9ES53
• Cytogenetics: 11q23
• MW: 34.5 kDa
• Gene Summary: Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (PubMed:10811609). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).[UniProtKB/Swiss-Prot Function]