D2hgdh (NM_001106926) Rat Tagged ORF Clone

CAT#: RR208309

D2hgdh (Myc-DDK-tagged ORF) - Rat D-2-hydroxyglutarate dehydrogenase (D2hgdh), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D2hgdh
• Synonyms: RGD1307976
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208309 representing NM_001106926
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCCATTTGGTGCCCAGATGGTCTGCAAGCCTCTTCAGGGCATCTCCAAGATGGAAAAAGACCT
  ACAGCCAGAGAGCTTCTGCGCAGTTGAAGTGGCTGGGTTGCCCTCGGAGTGTGTACAGCCCCTTGGCTTG
  CAGAGCATACTCCAAGGTCTCTGGCAGCCCTGAGGTGATGCTGACCCCTGAGCGATACCCAGTGCAACGG
  CTGCCGTTTTCTACCGTGTCAGAGGAGGACCTGGCTGCATTTGAATGCATCATACCTGGCAGAGTTATCA
  CAGATCCAGAGCAACTGCAGACTTGCAATGTGGACTGGCTGAGGACCGTGCGGGGCTGTAGTAAGGTGCT
  GCTGAGACCCCAGACCTCAGAGGAAGTCTCCCAGATTCTTAGGCACTGCTATAAGAGGAACTTGGCTGTG
  AACCCACAGGGCGGAAACACTGGCATGGTGGGTGGCAGTGTCCCTGTCTTTGATGAAGTCATCCTGTCCA
  CTGCCCTCATGAATCAAGTCATCAGCTTTCATGATGTGTCTGGAATTCTGGTTTGCCAGGCAGGCTGCGT
  GCTGGAGGAGCTGAGCAGGTACGTGCAGGAGCGGGACTTCATCATGCCACTGGACTTGGGTGCAAAGGGC
  AGCTGCCACATTGGGGGTAATGTCGCAACGAACGCTGGGGGTCTGCGGTTTCTGCGGTACGGCTCTCTTC
  GAGGGACTGTCCTGGGCCTGGAAGTGGTACTTGCTGATGGAACCATCCTGAACTGCCTGACCTCTTTGAG
  GAAGGACAACACTGGCTATGACCTGAAGCAGATGTTCATTGGGTCAGAGGGCACCCTGGGAGTCATCACT
  GCTGTATCCATCGTGTGCCCACCGAGGCCCAAGGCTGTGAATGTGGCTTTCCTTGGCTGCCCTGGGTTTA
  CTGAGGTTCTGCAGACCTTCTGCACCTGCAAGGGGCAACTGGGCGAGATCCTGTCTGCCTTTGAGTTTAT
  GGATGCCGAATGCATGCAGTTGGTTGGACAGCACCTCCACCTGACCAACCCAGTACAAGAGAGTCCATTC
  TATGTCCTGGTTGAGACCTCAGGCTCCAGTGCCGGGCACGATGCTGAGAAACTGACCAACGTCCTGGAGC
  AAGTGCTGAATTCTGGCCTGGTGATCGATGGCACTATGGCCACCGACCAGAGGAAAGTCCAGATGCTGTG
  GGCCTTGCGGGAAAGGATCACAGAGGCACTCAGTCGTGACGGCTACGTGTTCAAGTATGACTTATCCCTC
  CCCGTGGAGCGGCTCTATGACCTTGTGATCGACCTGCGCACCCGCCTTGGCCCTCGAGCCAAGCATGTGG
  TGGGATATGGGCACTTGGGGGATGGCAACCTTCACCTGAACGTGACAGCAGAGGCCTTTAGCCAGGAGCT
  GCTTGGTGCCTTGGAGCCTTATGTGTACGCCTGGACAGCGGAACAGCGGGGCAGCGTCAGTGCTGAGCAC
  GGCCTGGGCTTCAAGAAGAAGAATGTACTCGGCTATAGCAACGCCACCTGTGGCTGTGAAGCTTATGCAA
  CAGCTGAAGGCCATGCTGGACCCCAAAGGCATCCTGAACCCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208309 representing NM_001106926
  Red=Cloning site Green=Tags(s)
  
  MVLHLVPRWSASLFRASPRWKKTYSQRASAQLKWLGCPRSVYSPLACRAYSKVSGSPEVMLTPERYPVQR
  LPFSTVSEEDLAAFECIIPGRVITDPEQLQTCNVDWLRTVRGCSKVLLRPQTSEEVSQILRHCYKRNLAV
  NPQGGNTGMVGGSVPVFDEVILSTALMNQVISFHDVSGILVCQAGCVLEELSRYVQERDFIMPLDLGAKG
  SCHIGGNVATNAGGLRFLRYGSLRGTVLGLEVVLADGTILNCLTSLRKDNTGYDLKQMFIGSEGTLGVIT
  AVSIVCPPRPKAVNVAFLGCPGFTEVLQTFCTCKGQLGEILSAFEFMDAECMQLVGQHLHLTNPVQESPF
  YVLVETSGSSAGHDAEKLTNVLEQVLNSGLVIDGTMATDQRKVQMLWALRERITEALSRDGYVFKYDLSL
  PVERLYDLVIDLRTRLGPRAKHVVGYGHLGDGNLHLNVTAEAFSQELLGALEPYVYAWTAEQRGSVSAEH
  GLGFKKKNVLGYSNATCGCEAYATAEGHAGPQRHPEPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106926
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106926.1, NP_001100396.1
• RefSeq Size: 2279 bp
• RefSeq ORF: 1587 bp
• Locus ID: 301624
• Cytogenetics: 9q36
• MW: 57.8 kDa
• Gene Summary: Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.[UniProtKB/Swiss-Prot Function]