Cbs (NM_012522) Rat Tagged ORF Clone

CAT#: RR208308

Cbs (Myc-DDK-tagged ORF) - Rat cystathionine beta synthase (Cbs), (10 ug)

Product Images

Circular map for RR208308

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208308 representing NM_012522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCAGGGACATCCCAGTGTGAAGATGGCTCTGCAGGGTGCCCCCAGGACTTGGAGGTACAGCCAG
  AAAAAGGGCAACTGGAGAAGGGAGCCTCAGGGGACAAGGAAAGAGTCTGGATCTCGCCTGATACCCCAAG
  CAGATGTACTTGGCAGCTGGGCAGGCCCATGGCGGATTCCCCACATTACCACACAGTGCCGACAAAATCC
  CCGAAAATTTTGCCAGATATTCTGAGGAAAATTGGCAACACCCCTATGGTCAGAATCAACAGGATCTCCA
  AGAATGCAGGACTCAAGTGCGAGCTGTTGGCCAAGTGTGAGTTCTTCAACGCCGGTGGGAGTGTGAAGGA
  CCGCATCAGCCTCCGGATGATTGAAGACGCTGAGCGAGCCGGAACCTTGAAGCCCGGAGACACGATCATT
  GAGCCAACTTCTGGCAACACAGGGATCGGGCTGGCTCTGGCAGCTGCTGTGAAGGGCTATCGCTGCATTA
  TCGTGATGCCTGAGAAGATGAGTATGGAGAAGGTGGATGTGCTGCGAGCTCTGGGAGCTGAGATTGTGAG
  GACGCCCACCAACGCCAGATTCGATTCCCCCGAGTCCCACGTAGGAGTGGCATGGCGACTGAAGAACGAA
  ATCCCCAATTCTCACATTCTGGACCAGTACCGCAATGCCAGCAACCCCTTGGCGCACTACGATGACACCG
  CAGAGGAGATCCTGCAGCAGTGCGACGGGAAGGTGGACATGCTGGTGGCTTCAGCAGGCACGGGTGGCAC
  CATCACGGGTATCGCGAGGAAGCTGAAGGAGAAGTGCCCAGGTTGTAAAATCATCGGTGTAGATCCCGAG
  GGGTCCATCCTCGCGGAGCCCGAGGAGCTGAACCAGACGGAGCAAACAGCCTATGAGGTGGAAGGGATCG
  GCTACGACTTCATCCCCACCGTCCTGGACAGGGCGGTGGTGGATAGGTGGTTCAAGAGCAATGATGACGA
  TTCCTTCGCCTTCGCCCGCATGCTCATCTCCCAGGAGGGACTGCTGTGCGGTGGGAGTTCAGGCAGCGCT
  ATGGCCGTGGCTGTGAAGGCTGCCCAGGAGCTAAAGGAAGGACAGCGCTGTGTGGTCATCCTGCCCGACT
  CTGTGCGCAACTACATGTCCAAGTTCTTGAGTGACAAATGGATGCTGCAGAAAGGCTTCATGAAGGAGGA
  GCTCTCCGTGAAGAGACCCTGGTGGTGGCATCTGCGTGTCCAAGAGCTGAGCCTATCAGCACCGCTGACC
  GTGTTGCCCACTGTCACCTGTGAGCACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACCTG
  TGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACTCTCGGGAACATGTTGTCCTCCCTGCTTGCTGG
  GAAGGTGCGGCCATCAGACGAAGTCTGCAAAGTCCTCTACAAGCAGTTCAAGCCGATCCACCTGACCGAC
  ACACTGGGCATGCTCTCCCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTCCATGAGCAGATCCAAT
  ACCGCAACAATGGCGTGTCCAGCAAGCAGCTGATGGTGTTTGGTGTTGTCACCGCCATTGACCTGCTAAA
  CTTCGTGGCAGCCCGTGAGCAGACCCGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208308 representing NM_012522
  Red=Cloning site Green=Tags(s)
  
  MPSGTSQCEDGSAGCPQDLEVQPEKGQLEKGASGDKERVWISPDTPSRCTWQLGRPMADSPHYHTVPTKS
  PKILPDILRKIGNTPMVRINRISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGTLKPGDTII
  EPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE
  IPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKVDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPE
  GSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDRWFKSNDDDSFAFARMLISQEGLLCGGSSGSA
  MAVAVKAAQELKEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWHLRVQELSLSAPLT
  VLPTVTCEHTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTD
  TLGMLSHILEMDHFALVVHEQIQYRNNGVSSKQLMVFGVVTAIDLLNFVAAREQTRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012522
• ORF Size: 1641 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012522.2, NP_036654.2
• RefSeq Size: 2539 bp
• RefSeq ORF: 1644 bp
• Locus ID: 24250
• UniProt ID: P32232
• Cytogenetics: 20p12
• MW: 60.1 kDa
• Gene Summary: catalyzes condensation of serine and homocyteine to cystathione; involved in transsulfuration pathway; regulated by insulin; alternative splicing produces four mRNA species [RGD, Feb 2006]